Experiment: KB with Terpene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0781 and Psyr_0782 overlap by 38 nucleotides Psyr_0782 and Psyr_0783 overlap by 4 nucleotides Psyr_0783 and Psyr_0784 are separated by 9 nucleotides
Psyr_0781: Psyr_0781 - Protein-glutamate methylesterase, at 887,924 to 889,000
_0781
Psyr_0782: Psyr_0782 - CheD, at 888,963 to 889,496
_0782
Psyr_0783: Psyr_0783 - MCP methyltransferase, CheR-type, at 889,493 to 890,302
_0783
Psyr_0784: Psyr_0784 - CheW-like protein, at 890,312 to 890,851
_0784
Position (kb) 888
889
890 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 888.219 kb on + strand, within Psyr_0781 at 888.220 kb on - strand, within Psyr_0781 at 888.220 kb on - strand, within Psyr_0781 at 888.220 kb on - strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.222 kb on + strand, within Psyr_0781 at 888.223 kb on - strand, within Psyr_0781 at 888.223 kb on - strand, within Psyr_0781 at 888.614 kb on + strand, within Psyr_0781 at 888.879 kb on - strand, within Psyr_0781 at 888.941 kb on + strand at 888.953 kb on + strand at 888.953 kb on + strand at 888.977 kb on + strand at 889.038 kb on + strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.039 kb on - strand, within Psyr_0782 at 889.232 kb on - strand, within Psyr_0782 at 889.275 kb on + strand, within Psyr_0782 at 889.275 kb on + strand, within Psyr_0782 at 889.276 kb on - strand, within Psyr_0782 at 889.297 kb on + strand, within Psyr_0782 at 889.467 kb on + strand at 889.467 kb on + strand at 889.468 kb on - strand at 889.468 kb on - strand at 889.477 kb on - strand at 889.578 kb on + strand, within Psyr_0783 at 889.578 kb on + strand, within Psyr_0783 at 889.578 kb on + strand, within Psyr_0783 at 889.578 kb on + strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.579 kb on - strand, within Psyr_0783 at 889.644 kb on + strand, within Psyr_0783 at 889.644 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.794 kb on + strand, within Psyr_0783 at 889.795 kb on - strand, within Psyr_0783 at 889.795 kb on - strand, within Psyr_0783 at 889.837 kb on + strand, within Psyr_0783 at 889.947 kb on + strand, within Psyr_0783 at 890.064 kb on + strand, within Psyr_0783 at 890.064 kb on + strand, within Psyr_0783 at 890.065 kb on - strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.151 kb on + strand, within Psyr_0783 at 890.167 kb on - strand, within Psyr_0783 at 890.244 kb on + strand at 890.245 kb on - strand at 890.293 kb on + strand at 890.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Terpene 10 mM remove 888,219 + Psyr_0781 0.27 +0.7 888,220 - Psyr_0781 0.27 -0.8 888,220 - Psyr_0781 0.27 -0.6 888,220 - Psyr_0781 0.27 -0.1 888,222 + Psyr_0781 0.28 +0.2 888,222 + Psyr_0781 0.28 -0.9 888,222 + Psyr_0781 0.28 +1.1 888,222 + Psyr_0781 0.28 -0.0 888,223 - Psyr_0781 0.28 +0.8 888,223 - Psyr_0781 0.28 +0.6 888,614 + Psyr_0781 0.64 +0.0 888,879 - Psyr_0781 0.89 -3.3 888,941 + -1.1 888,953 + +0.5 888,953 + -0.9 888,977 + +0.2 889,038 + Psyr_0782 0.14 -0.0 889,039 - Psyr_0782 0.14 +0.8 889,039 - Psyr_0782 0.14 +0.4 889,039 - Psyr_0782 0.14 -0.3 889,039 - Psyr_0782 0.14 +0.1 889,232 - Psyr_0782 0.50 +0.3 889,275 + Psyr_0782 0.58 +2.6 889,275 + Psyr_0782 0.58 +1.2 889,276 - Psyr_0782 0.59 +0.2 889,297 + Psyr_0782 0.63 -0.1 889,467 + -1.2 889,467 + +0.8 889,468 - -0.8 889,468 - -1.1 889,477 - +0.1 889,578 + Psyr_0783 0.10 -0.3 889,578 + Psyr_0783 0.10 -0.9 889,578 + Psyr_0783 0.10 -1.2 889,578 + Psyr_0783 0.10 -0.2 889,579 - Psyr_0783 0.11 -0.1 889,579 - Psyr_0783 0.11 -0.1 889,579 - Psyr_0783 0.11 +0.2 889,579 - Psyr_0783 0.11 +1.4 889,579 - Psyr_0783 0.11 -0.3 889,644 + Psyr_0783 0.19 -0.7 889,644 + Psyr_0783 0.19 +1.4 889,794 + Psyr_0783 0.37 +0.5 889,794 + Psyr_0783 0.37 -0.8 889,794 + Psyr_0783 0.37 -0.3 889,794 + Psyr_0783 0.37 +0.1 889,794 + Psyr_0783 0.37 +2.0 889,795 - Psyr_0783 0.37 -0.6 889,795 - Psyr_0783 0.37 -0.4 889,837 + Psyr_0783 0.42 +0.9 889,947 + Psyr_0783 0.56 +0.2 890,064 + Psyr_0783 0.70 +0.4 890,064 + Psyr_0783 0.70 +1.5 890,065 - Psyr_0783 0.71 +1.2 890,151 + Psyr_0783 0.81 -0.3 890,151 + Psyr_0783 0.81 -0.6 890,151 + Psyr_0783 0.81 -2.3 890,151 + Psyr_0783 0.81 +0.1 890,151 + Psyr_0783 0.81 -0.1 890,151 + Psyr_0783 0.81 +1.4 890,167 - Psyr_0783 0.83 +0.3 890,244 + -1.3 890,245 - -0.3 890,293 + -2.1 890,293 + -0.2
Or see this region's nucleotide sequence