Experiment: KB with Piperine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0545 and Psyr_0546 overlap by 32 nucleotides Psyr_0546 and Psyr_0547 are separated by 177 nucleotides Psyr_0547 and Psyr_0548 overlap by 10 nucleotides
Psyr_0545: Psyr_0545 - Permease for cytosine/purines, uracil, thiamine, allantoin, at 617,494 to 618,783
_0545
Psyr_0546: Psyr_0546 - lipoprotein, putative, at 618,752 to 619,498
_0546
Psyr_0547: Psyr_0547 - Conserved hypothetical protein 52, at 619,676 to 620,323
_0547
Psyr_0548: Psyr_0548 - Protein of unknown function DUF1249, at 620,314 to 620,766
_0548
Position (kb) 618
619
620 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 618.170 kb on - strand, within Psyr_0545 at 618.266 kb on - strand, within Psyr_0545 at 618.469 kb on - strand, within Psyr_0545 at 618.502 kb on + strand, within Psyr_0545 at 618.518 kb on + strand, within Psyr_0545 at 618.534 kb on - strand, within Psyr_0545 at 618.658 kb on + strand at 618.658 kb on + strand at 618.659 kb on - strand at 618.659 kb on - strand at 618.862 kb on - strand, within Psyr_0546 at 618.862 kb on - strand, within Psyr_0546 at 618.883 kb on - strand, within Psyr_0546 at 618.883 kb on - strand, within Psyr_0546 at 619.065 kb on + strand, within Psyr_0546 at 619.065 kb on + strand, within Psyr_0546 at 619.066 kb on - strand, within Psyr_0546 at 619.116 kb on + strand, within Psyr_0546 at 619.117 kb on - strand, within Psyr_0546 at 619.117 kb on - strand, within Psyr_0546 at 619.136 kb on + strand, within Psyr_0546 at 619.176 kb on + strand, within Psyr_0546 at 619.177 kb on - strand, within Psyr_0546 at 619.177 kb on - strand, within Psyr_0546 at 619.177 kb on - strand, within Psyr_0546 at 619.222 kb on - strand, within Psyr_0546 at 619.537 kb on + strand at 619.537 kb on + strand at 619.537 kb on + strand at 619.537 kb on + strand at 619.537 kb on + strand at 619.537 kb on + strand at 619.537 kb on + strand at 619.537 kb on + strand at 619.538 kb on - strand at 619.538 kb on - strand at 619.538 kb on - strand at 619.538 kb on - strand at 619.538 kb on - strand at 619.541 kb on + strand at 619.542 kb on - strand at 619.542 kb on - strand at 619.542 kb on - strand at 619.542 kb on - strand at 619.561 kb on + strand at 619.561 kb on + strand at 619.561 kb on + strand at 619.561 kb on + strand at 619.561 kb on + strand at 619.593 kb on + strand at 619.593 kb on + strand at 619.594 kb on - strand at 619.605 kb on - strand at 619.605 kb on - strand at 619.749 kb on + strand, within Psyr_0547 at 619.778 kb on + strand, within Psyr_0547 at 619.778 kb on + strand, within Psyr_0547 at 619.778 kb on + strand, within Psyr_0547 at 619.778 kb on + strand, within Psyr_0547 at 619.778 kb on + strand, within Psyr_0547 at 619.779 kb on - strand, within Psyr_0547 at 619.779 kb on - strand, within Psyr_0547 at 619.779 kb on - strand, within Psyr_0547 at 619.779 kb on - strand, within Psyr_0547 at 619.781 kb on - strand, within Psyr_0547 at 619.887 kb on - strand, within Psyr_0547 at 619.887 kb on - strand, within Psyr_0547 at 619.933 kb on - strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.081 kb on + strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.082 kb on - strand, within Psyr_0547 at 620.305 kb on - strand at 620.314 kb on - strand at 620.327 kb on + strand at 620.327 kb on + strand at 620.328 kb on - strand at 620.328 kb on - strand at 620.386 kb on + strand, within Psyr_0548 at 620.387 kb on - strand, within Psyr_0548 at 620.387 kb on - strand, within Psyr_0548 at 620.477 kb on + strand, within Psyr_0548
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Piperine 10 mM remove 618,170 - Psyr_0545 0.52 +0.6 618,266 - Psyr_0545 0.60 +0.4 618,469 - Psyr_0545 0.76 +0.2 618,502 + Psyr_0545 0.78 -0.9 618,518 + Psyr_0545 0.79 +0.5 618,534 - Psyr_0545 0.81 +2.4 618,658 + -1.7 618,658 + +3.4 618,659 - -0.7 618,659 - -0.3 618,862 - Psyr_0546 0.15 -0.9 618,862 - Psyr_0546 0.15 -2.0 618,883 - Psyr_0546 0.18 +0.6 618,883 - Psyr_0546 0.18 -2.2 619,065 + Psyr_0546 0.42 -0.4 619,065 + Psyr_0546 0.42 +1.9 619,066 - Psyr_0546 0.42 +0.1 619,116 + Psyr_0546 0.49 +0.5 619,117 - Psyr_0546 0.49 -0.9 619,117 - Psyr_0546 0.49 -0.4 619,136 + Psyr_0546 0.51 -1.6 619,176 + Psyr_0546 0.57 +1.1 619,177 - Psyr_0546 0.57 +0.2 619,177 - Psyr_0546 0.57 -0.5 619,177 - Psyr_0546 0.57 -0.2 619,222 - Psyr_0546 0.63 +0.3 619,537 + +0.1 619,537 + +1.4 619,537 + -0.6 619,537 + +0.2 619,537 + -1.9 619,537 + +0.8 619,537 + +0.1 619,537 + +0.9 619,538 - -0.1 619,538 - +0.9 619,538 - +0.8 619,538 - -0.3 619,538 - -1.8 619,541 + -1.3 619,542 - -1.0 619,542 - +0.5 619,542 - -2.9 619,542 - +2.7 619,561 + +0.6 619,561 + +1.1 619,561 + -1.7 619,561 + +0.8 619,561 + -1.9 619,593 + -0.4 619,593 + +0.1 619,594 - +1.1 619,605 - -0.5 619,605 - -0.5 619,749 + Psyr_0547 0.11 -0.6 619,778 + Psyr_0547 0.16 +1.2 619,778 + Psyr_0547 0.16 +2.7 619,778 + Psyr_0547 0.16 +1.5 619,778 + Psyr_0547 0.16 -1.9 619,778 + Psyr_0547 0.16 -2.1 619,779 - Psyr_0547 0.16 -0.8 619,779 - Psyr_0547 0.16 +2.8 619,779 - Psyr_0547 0.16 -0.7 619,779 - Psyr_0547 0.16 +0.0 619,781 - Psyr_0547 0.16 -3.2 619,887 - Psyr_0547 0.33 -0.0 619,887 - Psyr_0547 0.33 +0.1 619,933 - Psyr_0547 0.40 +3.4 620,081 + Psyr_0547 0.62 +1.1 620,081 + Psyr_0547 0.62 +1.1 620,081 + Psyr_0547 0.62 +0.6 620,081 + Psyr_0547 0.62 +2.3 620,081 + Psyr_0547 0.62 -0.4 620,081 + Psyr_0547 0.62 -1.0 620,082 - Psyr_0547 0.63 +0.7 620,082 - Psyr_0547 0.63 +0.0 620,082 - Psyr_0547 0.63 -0.7 620,082 - Psyr_0547 0.63 +1.1 620,305 - -0.2 620,314 - +1.5 620,327 + +0.8 620,327 + -1.3 620,328 - +2.1 620,328 - +3.1 620,386 + Psyr_0548 0.16 +0.1 620,387 - Psyr_0548 0.16 +3.9 620,387 - Psyr_0548 0.16 +0.4 620,477 + Psyr_0548 0.36 -0.8
Or see this region's nucleotide sequence