Experiment: Acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfeR and yfeH are separated by 89 nucleotides yfeH and ypeB overlap by 4 nucleotides ypeB and ligA are separated by 1 nucleotides
b2409: yfeR - predicted DNA-binding transcriptional regulator (NCBI), at 2,523,952 to 2,524,878
yfeR
b2410: yfeH - predicted inner membrane protein (NCBI), at 2,524,968 to 2,525,966
yfeH
b4546: ypeB - hypothetical protein (NCBI), at 2,525,963 to 2,526,181
ypeB
b2411: ligA - NAD-dependent DNA ligase LigA (NCBI), at 2,526,183 to 2,528,198
ligA
Position (kb)
2524
2525
2526 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2523.972 kb on - strand at 2524.005 kb on + strand at 2524.006 kb on + strand at 2524.006 kb on + strand at 2524.231 kb on - strand, within yfeR at 2524.422 kb on + strand, within yfeR at 2524.481 kb on - strand, within yfeR at 2524.614 kb on + strand, within yfeR at 2524.636 kb on - strand, within yfeR at 2524.636 kb on - strand, within yfeR at 2524.653 kb on - strand, within yfeR at 2524.653 kb on - strand, within yfeR at 2524.788 kb on + strand at 2524.794 kb on + strand at 2524.794 kb on + strand at 2524.802 kb on - strand at 2524.941 kb on - strand at 2524.941 kb on - strand at 2525.116 kb on + strand, within yfeH at 2525.248 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.454 kb on - strand, within yfeH at 2525.613 kb on + strand, within yfeH at 2525.709 kb on + strand, within yfeH at 2525.727 kb on - strand, within yfeH at 2525.727 kb on - strand, within yfeH at 2525.887 kb on + strand at 2525.942 kb on + strand at 2526.092 kb on + strand, within ypeB
Per-strain Table
Position Strand Gene LocusTag Fraction Acetate (C) remove 2,523,972 - +0.0 2,524,005 + +0.8 2,524,006 + +0.5 2,524,006 + +1.5 2,524,231 - yfeR b2409 0.30 +0.4 2,524,422 + yfeR b2409 0.51 +0.1 2,524,481 - yfeR b2409 0.57 -0.6 2,524,614 + yfeR b2409 0.71 -0.0 2,524,636 - yfeR b2409 0.74 +1.7 2,524,636 - yfeR b2409 0.74 -0.2 2,524,653 - yfeR b2409 0.76 +0.1 2,524,653 - yfeR b2409 0.76 -0.7 2,524,788 + -2.6 2,524,794 + +1.8 2,524,794 + +0.6 2,524,802 - +1.7 2,524,941 - +0.9 2,524,941 - -0.4 2,525,116 + yfeH b2410 0.15 -1.2 2,525,248 - yfeH b2410 0.28 -2.1 2,525,454 - yfeH b2410 0.49 -0.9 2,525,454 - yfeH b2410 0.49 -1.8 2,525,454 - yfeH b2410 0.49 -2.4 2,525,454 - yfeH b2410 0.49 -1.3 2,525,613 + yfeH b2410 0.65 -0.8 2,525,709 + yfeH b2410 0.74 -1.3 2,525,727 - yfeH b2410 0.76 -1.5 2,525,727 - yfeH b2410 0.76 -0.9 2,525,887 + -4.5 2,525,942 + -1.2 2,526,092 + ypeB b4546 0.59 +0.5
Or see this region's nucleotide sequence