Experiment: Acetate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dadX and b1191 are separated by 385 nucleotides b1191 and ldcA are separated by 94 nucleotides
b1190: dadX - alanine racemase (NCBI), at 1,238,102 to 1,239,172
dadX
b1191: b1191 - orf, hypothetical protein (VIMSS), at 1,239,558 to 1,241,294
b1191
b1192: ldcA - L,D-carboxypeptidase A (NCBI), at 1,241,389 to 1,242,303
ldcA
Position (kb)
1239
1240
1241
1242 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1238.803 kb on + strand, within dadX at 1238.803 kb on + strand, within dadX at 1238.855 kb on + strand, within dadX at 1238.874 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX at 1239.281 kb on - strand at 1239.422 kb on - strand at 1239.639 kb on - strand at 1239.674 kb on + strand at 1239.674 kb on + strand at 1239.690 kb on - strand at 1239.769 kb on + strand, within b1191 at 1239.842 kb on + strand, within b1191 at 1239.886 kb on + strand, within b1191 at 1239.886 kb on + strand, within b1191 at 1239.977 kb on + strand, within b1191 at 1240.277 kb on - strand, within b1191 at 1240.277 kb on - strand, within b1191 at 1240.277 kb on - strand, within b1191 at 1240.352 kb on + strand, within b1191 at 1240.364 kb on + strand at 1240.364 kb on + strand, within b1191 at 1240.372 kb on - strand, within b1191 at 1240.372 kb on - strand, within b1191 at 1240.433 kb on + strand, within b1191 at 1240.506 kb on + strand, within b1191 at 1240.552 kb on - strand, within b1191 at 1240.627 kb on - strand, within b1191 at 1240.675 kb on - strand, within b1191 at 1240.750 kb on - strand, within b1191 at 1240.974 kb on + strand, within b1191 at 1240.995 kb on - strand, within b1191 at 1240.997 kb on - strand, within b1191 at 1241.032 kb on - strand, within b1191 at 1241.068 kb on + strand, within b1191 at 1241.102 kb on - strand, within b1191 at 1241.102 kb on - strand, within b1191 at 1241.112 kb on + strand, within b1191 at 1241.143 kb on - strand at 1241.159 kb on - strand at 1241.159 kb on - strand at 1241.231 kb on + strand at 1241.231 kb on + strand at 1241.331 kb on + strand at 1241.331 kb on + strand at 1241.424 kb on + strand at 1241.425 kb on - strand at 1241.433 kb on + strand at 1241.501 kb on + strand, within ldcA at 1241.572 kb on - strand, within ldcA at 1241.581 kb on + strand, within ldcA at 1241.747 kb on + strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.999 kb on - strand, within ldcA at 1242.033 kb on + strand, within ldcA at 1242.208 kb on - strand, within ldcA at 1242.237 kb on - strand at 1242.284 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acetate (C) remove 1,238,803 + dadX b1190 0.65 +0.9 1,238,803 + dadX b1190 0.65 +0.1 1,238,855 + dadX b1190 0.70 +0.4 1,238,874 - dadX b1190 0.72 +0.5 1,238,920 - dadX b1190 0.76 +1.3 1,238,920 - dadX b1190 0.76 +2.8 1,239,281 - -0.6 1,239,422 - -0.3 1,239,639 - -0.8 1,239,674 + +2.1 1,239,674 + +1.9 1,239,690 - +1.7 1,239,769 + b1191 b1191 0.12 +1.9 1,239,842 + b1191 b1191 0.16 +1.0 1,239,886 + b1191 b1191 0.19 +1.9 1,239,886 + b1191 b1191 0.19 +1.5 1,239,977 + b1191 b1191 0.24 +1.7 1,240,277 - b1191 b1191 0.41 +1.6 1,240,277 - b1191 b1191 0.41 +1.8 1,240,277 - b1191 b1191 0.41 +1.6 1,240,352 + b1191 b1191 0.46 +1.0 1,240,364 + +2.5 1,240,364 + b1191 b1191 0.46 +2.4 1,240,372 - b1191 b1191 0.47 +1.2 1,240,372 - b1191 b1191 0.47 +1.7 1,240,433 + b1191 b1191 0.50 +2.6 1,240,506 + b1191 b1191 0.55 +1.6 1,240,552 - b1191 b1191 0.57 +1.9 1,240,627 - b1191 b1191 0.62 +1.7 1,240,675 - b1191 b1191 0.64 +2.1 1,240,750 - b1191 b1191 0.69 +6.6 1,240,974 + b1191 b1191 0.82 +2.5 1,240,995 - b1191 b1191 0.83 +1.9 1,240,997 - b1191 b1191 0.83 +2.0 1,241,032 - b1191 b1191 0.85 +1.9 1,241,068 + b1191 b1191 0.87 +1.2 1,241,102 - b1191 b1191 0.89 +1.0 1,241,102 - b1191 b1191 0.89 +2.0 1,241,112 + b1191 b1191 0.89 +1.5 1,241,143 - +1.9 1,241,159 - +2.4 1,241,159 - +2.1 1,241,231 + +1.2 1,241,231 + +2.1 1,241,331 + +1.6 1,241,331 + +0.7 1,241,424 + -4.8 1,241,425 - -3.0 1,241,433 + -3.6 1,241,501 + ldcA b1192 0.12 -5.3 1,241,572 - ldcA b1192 0.20 -3.9 1,241,581 + ldcA b1192 0.21 -2.3 1,241,747 + ldcA b1192 0.39 -1.0 1,241,936 - ldcA b1192 0.60 -2.9 1,241,936 - ldcA b1192 0.60 -4.6 1,241,961 - ldcA b1192 0.63 -2.7 1,241,961 - ldcA b1192 0.63 -2.9 1,241,999 - ldcA b1192 0.67 -2.1 1,242,033 + ldcA b1192 0.70 -3.6 1,242,208 - ldcA b1192 0.90 -1.6 1,242,237 - -2.7 1,242,284 + -1.8
Or see this region's nucleotide sequence