Strain Fitness in Escherichia coli BW25113 around b2829

Experiment: Acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlgt and ptsP are separated by 150 nucleotidesptsP and nudH are separated by 12 nucleotides b2828: lgt - prolipoprotein diacylglyceryl transferase (NCBI), at 2,963,184 to 2,964,059 lgt b2829: ptsP - fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI), at 2,964,210 to 2,966,456 ptsP b2830: nudH - dinucleoside polyphosphate hydrolase (NCBI), at 2,966,469 to 2,966,999 nudH Position (kb) 2964 2965 2966 2967Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 2963.239 kb on - strandat 2964.140 kb on - strandat 2964.199 kb on - strandat 2964.208 kb on + strandat 2964.210 kb on + strandat 2964.234 kb on + strandat 2964.280 kb on - strandat 2964.296 kb on + strandat 2964.368 kb on + strandat 2964.382 kb on + strandat 2964.382 kb on + strandat 2964.401 kb on + strandat 2964.417 kb on - strandat 2964.419 kb on - strandat 2964.462 kb on - strand, within ptsPat 2964.524 kb on + strand, within ptsPat 2964.524 kb on + strand, within ptsPat 2964.543 kb on - strand, within ptsPat 2964.575 kb on - strand, within ptsPat 2964.589 kb on + strand, within ptsPat 2964.634 kb on - strand, within ptsPat 2964.653 kb on - strand, within ptsPat 2964.665 kb on - strand, within ptsPat 2964.665 kb on - strand, within ptsPat 2964.775 kb on + strand, within ptsPat 2964.782 kb on - strand, within ptsPat 2964.783 kb on - strand, within ptsPat 2964.825 kb on - strand, within ptsPat 2964.825 kb on - strand, within ptsPat 2964.997 kb on - strand, within ptsPat 2964.998 kb on - strand, within ptsPat 2965.011 kb on - strand, within ptsPat 2965.011 kb on - strand, within ptsPat 2965.081 kb on - strand, within ptsPat 2965.081 kb on - strand, within ptsPat 2965.158 kb on - strand, within ptsPat 2965.249 kb on - strand, within ptsPat 2965.312 kb on + strand, within ptsPat 2965.360 kb on - strand, within ptsPat 2965.360 kb on - strand, within ptsPat 2965.393 kb on - strand, within ptsPat 2965.405 kb on - strand, within ptsPat 2965.443 kb on - strand, within ptsPat 2965.443 kb on - strand, within ptsPat 2965.575 kb on - strand, within ptsPat 2965.575 kb on - strand, within ptsPat 2965.860 kb on + strand, within ptsPat 2965.876 kb on + strand, within ptsPat 2965.910 kb on - strand, within ptsPat 2965.937 kb on - strand, within ptsPat 2965.937 kb on - strand, within ptsPat 2965.968 kb on + strand, within ptsPat 2965.971 kb on + strand, within ptsPat 2966.002 kb on + strand, within ptsPat 2966.055 kb on - strand, within ptsPat 2966.063 kb on + strandat 2966.063 kb on + strand, within ptsPat 2966.064 kb on + strand, within ptsPat 2966.089 kb on - strand, within ptsPat 2966.089 kb on - strand, within ptsPat 2966.167 kb on - strand, within ptsPat 2966.258 kb on + strandat 2966.346 kb on + strandat 2966.346 kb on + strandat 2966.346 kb on - strandat 2966.400 kb on + strandat 2966.459 kb on - strandat 2966.459 kb on - strandat 2966.465 kb on + strandat 2966.467 kb on + strandat 2966.467 kb on + strandat 2966.474 kb on - strandat 2966.484 kb on - strandat 2966.484 kb on - strandat 2966.532 kb on + strand, within nudHat 2966.565 kb on + strand, within nudHat 2966.645 kb on + strand, within nudHat 2966.645 kb on + strand, within nudHat 2966.722 kb on + strand, within nudHat 2967.071 kb on + strandat 2967.078 kb on + strandat 2967.078 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Acetate (C)
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2,963,239 - +0.5
2,964,140 - +1.2
2,964,199 - -0.1
2,964,208 + +1.0
2,964,210 + -1.3
2,964,234 + +3.0
2,964,280 - +3.0
2,964,296 + +2.6
2,964,368 + +2.0
2,964,382 + +2.7
2,964,382 + +3.0
2,964,401 + +2.0
2,964,417 - +3.1
2,964,419 - +3.1
2,964,462 - ptsP b2829 0.11 +3.1
2,964,524 + ptsP b2829 0.14 +2.4
2,964,524 + ptsP b2829 0.14 +2.6
2,964,543 - ptsP b2829 0.15 +2.8
2,964,575 - ptsP b2829 0.16 +2.9
2,964,589 + ptsP b2829 0.17 +2.4
2,964,634 - ptsP b2829 0.19 +2.8
2,964,653 - ptsP b2829 0.20 +2.7
2,964,665 - ptsP b2829 0.20 +3.0
2,964,665 - ptsP b2829 0.20 +3.3
2,964,775 + ptsP b2829 0.25 +3.4
2,964,782 - ptsP b2829 0.25 +2.8
2,964,783 - ptsP b2829 0.26 +2.4
2,964,825 - ptsP b2829 0.27 +2.9
2,964,825 - ptsP b2829 0.27 +2.9
2,964,997 - ptsP b2829 0.35 +2.9
2,964,998 - ptsP b2829 0.35 +3.4
2,965,011 - ptsP b2829 0.36 +2.9
2,965,011 - ptsP b2829 0.36 +3.2
2,965,081 - ptsP b2829 0.39 +3.0
2,965,081 - ptsP b2829 0.39 +2.9
2,965,158 - ptsP b2829 0.42 +2.8
2,965,249 - ptsP b2829 0.46 +2.7
2,965,312 + ptsP b2829 0.49 +2.5
2,965,360 - ptsP b2829 0.51 +2.9
2,965,360 - ptsP b2829 0.51 +2.7
2,965,393 - ptsP b2829 0.53 +2.8
2,965,405 - ptsP b2829 0.53 +3.3
2,965,443 - ptsP b2829 0.55 +3.2
2,965,443 - ptsP b2829 0.55 +2.9
2,965,575 - ptsP b2829 0.61 +2.5
2,965,575 - ptsP b2829 0.61 +2.7
2,965,860 + ptsP b2829 0.73 +2.3
2,965,876 + ptsP b2829 0.74 +2.4
2,965,910 - ptsP b2829 0.76 +2.8
2,965,937 - ptsP b2829 0.77 +2.4
2,965,937 - ptsP b2829 0.77 +4.9
2,965,968 + ptsP b2829 0.78 +2.0
2,965,971 + ptsP b2829 0.78 +2.3
2,966,002 + ptsP b2829 0.80 +1.8
2,966,055 - ptsP b2829 0.82 -3.4
2,966,063 + +3.5
2,966,063 + ptsP b2829 0.82 +2.5
2,966,064 + ptsP b2829 0.83 +2.0
2,966,089 - ptsP b2829 0.84 +1.7
2,966,089 - ptsP b2829 0.84 +1.4
2,966,167 - ptsP b2829 0.87 +2.5
2,966,258 + +2.4
2,966,346 + +2.0
2,966,346 + +1.9
2,966,346 - +2.1
2,966,400 + +1.0
2,966,459 - -0.9
2,966,459 - +0.6
2,966,465 + +1.0
2,966,467 + +0.5
2,966,467 + +1.4
2,966,474 - +1.1
2,966,484 - -0.2
2,966,484 - -1.1
2,966,532 + nudH b2830 0.12 -0.9
2,966,565 + nudH b2830 0.18 -1.4
2,966,645 + nudH b2830 0.33 -1.1
2,966,645 + nudH b2830 0.33 -0.0
2,966,722 + nudH b2830 0.48 -0.6
2,967,071 + +0.6
2,967,078 + +1.5
2,967,078 + -0.6

Or see this region's nucleotide sequence