Experiment: KB with Palmatine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2529 and Psyr_2530 overlap by 4 nucleotides Psyr_2530 and Psyr_2531 are separated by 188 nucleotides
Psyr_2529: Psyr_2529 - Amidohydrolase, at 2,940,346 to 2,941,605
_2529
Psyr_2530: Psyr_2530 - General substrate transporter:Major facilitator superfamily, at 2,941,602 to 2,942,912
_2530
Psyr_2531: Psyr_2531 - transcriptional regulator, LysR family, at 2,943,101 to 2,944,012
_2531
Position (kb)
2941
2942
2943 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2940.908 kb on + strand, within Psyr_2529 at 2940.908 kb on + strand, within Psyr_2529 at 2940.908 kb on + strand, within Psyr_2529 at 2940.909 kb on - strand, within Psyr_2529 at 2940.909 kb on - strand, within Psyr_2529 at 2940.909 kb on - strand, within Psyr_2529 at 2941.332 kb on - strand, within Psyr_2529 at 2941.486 kb on + strand at 2941.486 kb on + strand at 2941.487 kb on - strand at 2941.487 kb on - strand at 2941.699 kb on + strand at 2941.699 kb on + strand at 2941.699 kb on + strand at 2941.700 kb on - strand at 2941.792 kb on + strand, within Psyr_2530 at 2941.792 kb on + strand, within Psyr_2530 at 2941.867 kb on + strand, within Psyr_2530 at 2941.867 kb on + strand, within Psyr_2530 at 2941.867 kb on + strand, within Psyr_2530 at 2941.868 kb on - strand, within Psyr_2530 at 2942.131 kb on + strand, within Psyr_2530 at 2942.132 kb on - strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.254 kb on + strand, within Psyr_2530 at 2942.254 kb on + strand, within Psyr_2530 at 2942.255 kb on - strand, within Psyr_2530 at 2942.255 kb on - strand, within Psyr_2530 at 2942.255 kb on - strand, within Psyr_2530 at 2942.286 kb on - strand, within Psyr_2530 at 2942.287 kb on + strand, within Psyr_2530 at 2942.288 kb on - strand, within Psyr_2530 at 2942.368 kb on + strand, within Psyr_2530 at 2942.444 kb on - strand, within Psyr_2530 at 2942.564 kb on - strand, within Psyr_2530 at 2942.589 kb on - strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.741 kb on - strand, within Psyr_2530 at 2942.901 kb on + strand at 2942.901 kb on + strand at 2942.901 kb on + strand at 2942.902 kb on - strand at 2942.902 kb on - strand at 2942.935 kb on - strand at 2943.063 kb on + strand at 2943.064 kb on - strand at 2943.198 kb on + strand, within Psyr_2531 at 2943.201 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.258 kb on - strand, within Psyr_2531 at 2943.258 kb on - strand, within Psyr_2531
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine 10 mM remove 2,940,908 + Psyr_2529 0.45 +0.2 2,940,908 + Psyr_2529 0.45 +2.4 2,940,908 + Psyr_2529 0.45 -0.4 2,940,909 - Psyr_2529 0.45 -0.9 2,940,909 - Psyr_2529 0.45 -1.0 2,940,909 - Psyr_2529 0.45 +0.2 2,941,332 - Psyr_2529 0.78 -0.9 2,941,486 + -1.1 2,941,486 + -2.6 2,941,487 - -1.1 2,941,487 - +0.8 2,941,699 + -1.3 2,941,699 + -0.9 2,941,699 + -0.3 2,941,700 - -0.1 2,941,792 + Psyr_2530 0.14 +1.5 2,941,792 + Psyr_2530 0.14 +1.5 2,941,867 + Psyr_2530 0.20 -1.0 2,941,867 + Psyr_2530 0.20 +1.5 2,941,867 + Psyr_2530 0.20 -0.1 2,941,868 - Psyr_2530 0.20 +0.3 2,942,131 + Psyr_2530 0.40 -0.7 2,942,132 - Psyr_2530 0.40 -0.1 2,942,146 + Psyr_2530 0.41 -0.1 2,942,146 + Psyr_2530 0.41 +0.8 2,942,146 + Psyr_2530 0.41 +1.2 2,942,146 + Psyr_2530 0.41 -0.7 2,942,254 + Psyr_2530 0.50 +4.5 2,942,254 + Psyr_2530 0.50 -0.3 2,942,255 - Psyr_2530 0.50 -0.3 2,942,255 - Psyr_2530 0.50 -1.3 2,942,255 - Psyr_2530 0.50 +1.1 2,942,286 - Psyr_2530 0.52 +0.0 2,942,287 + Psyr_2530 0.52 +0.3 2,942,288 - Psyr_2530 0.52 +1.8 2,942,368 + Psyr_2530 0.58 -0.5 2,942,444 - Psyr_2530 0.64 -0.5 2,942,564 - Psyr_2530 0.73 +0.4 2,942,589 - Psyr_2530 0.75 +0.7 2,942,668 + Psyr_2530 0.81 +0.7 2,942,668 + Psyr_2530 0.81 +1.2 2,942,668 + Psyr_2530 0.81 -0.8 2,942,668 + Psyr_2530 0.81 +0.2 2,942,668 + Psyr_2530 0.81 -1.5 2,942,668 + Psyr_2530 0.81 -0.7 2,942,668 + Psyr_2530 0.81 +3.8 2,942,668 + Psyr_2530 0.81 -2.6 2,942,668 + Psyr_2530 0.81 +0.3 2,942,668 + Psyr_2530 0.81 +0.3 2,942,669 - Psyr_2530 0.81 -1.5 2,942,669 - Psyr_2530 0.81 -1.1 2,942,669 - Psyr_2530 0.81 -0.6 2,942,669 - Psyr_2530 0.81 -1.2 2,942,669 - Psyr_2530 0.81 +2.3 2,942,669 - Psyr_2530 0.81 +1.5 2,942,741 - Psyr_2530 0.87 +1.2 2,942,901 + -0.3 2,942,901 + -0.5 2,942,901 + +0.5 2,942,902 - -1.7 2,942,902 - -0.3 2,942,935 - +0.2 2,943,063 + +0.5 2,943,064 - +2.0 2,943,198 + Psyr_2531 0.11 +2.7 2,943,201 + Psyr_2531 0.11 -0.4 2,943,257 + Psyr_2531 0.17 +1.5 2,943,257 + Psyr_2531 0.17 -0.8 2,943,257 + Psyr_2531 0.17 -1.6 2,943,257 + Psyr_2531 0.17 +1.5 2,943,258 - Psyr_2531 0.17 +1.6 2,943,258 - Psyr_2531 0.17 -2.2
Or see this region's nucleotide sequence