Experiment: KB with Palmatine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1245 and Psyr_1246 are separated by 26 nucleotides Psyr_1246 and Psyr_1247 overlap by 1 nucleotides Psyr_1247 and Psyr_1248 are separated by 3 nucleotides
Psyr_1245: Psyr_1245 - 23S rRNA m(2)A-2503 methyltransferase, at 1,400,404 to 1,401,552
_1245
Psyr_1246: Psyr_1246 - TPR repeat protein:TPR repeat protein, at 1,401,579 to 1,402,337
_1246
Psyr_1247: Psyr_1247 - conserved hypothetical protein, at 1,402,337 to 1,403,341
_1247
Psyr_1248: Psyr_1248 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 1,403,345 to 1,404,454
_1248
Position (kb)
1402
1403
1404 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1401.390 kb on + strand, within Psyr_1245 at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.487 kb on + strand at 1401.488 kb on - strand at 1401.489 kb on + strand at 1401.489 kb on + strand at 1401.489 kb on + strand at 1401.490 kb on - strand at 1401.491 kb on + strand at 1401.491 kb on + strand at 1401.491 kb on + strand at 1401.491 kb on + strand at 1401.491 kb on + strand at 1401.491 kb on + strand at 1401.492 kb on - strand at 1401.492 kb on - strand at 1401.696 kb on + strand, within Psyr_1246 at 1401.696 kb on + strand, within Psyr_1246 at 1401.696 kb on + strand, within Psyr_1246 at 1401.909 kb on + strand, within Psyr_1246 at 1401.939 kb on + strand, within Psyr_1246 at 1402.149 kb on + strand, within Psyr_1246 at 1402.149 kb on + strand, within Psyr_1246 at 1402.149 kb on + strand, within Psyr_1246 at 1402.149 kb on + strand, within Psyr_1246 at 1402.149 kb on + strand, within Psyr_1246 at 1402.149 kb on + strand, within Psyr_1246 at 1402.149 kb on + strand, within Psyr_1246 at 1402.167 kb on + strand, within Psyr_1246 at 1402.170 kb on + strand, within Psyr_1246 at 1402.208 kb on + strand, within Psyr_1246 at 1402.208 kb on + strand, within Psyr_1246 at 1402.208 kb on + strand, within Psyr_1246 at 1402.213 kb on + strand, within Psyr_1246 at 1402.240 kb on + strand, within Psyr_1246 at 1402.240 kb on + strand, within Psyr_1246 at 1402.240 kb on + strand, within Psyr_1246 at 1402.240 kb on + strand, within Psyr_1246 at 1402.240 kb on + strand, within Psyr_1246 at 1402.240 kb on + strand, within Psyr_1246 at 1402.240 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.242 kb on + strand, within Psyr_1246 at 1402.311 kb on + strand at 1402.320 kb on + strand at 1402.320 kb on + strand at 1402.320 kb on + strand at 1402.320 kb on + strand at 1402.320 kb on + strand at 1402.513 kb on + strand, within Psyr_1247 at 1402.513 kb on + strand, within Psyr_1247 at 1402.529 kb on + strand, within Psyr_1247 at 1402.529 kb on + strand, within Psyr_1247 at 1402.857 kb on + strand, within Psyr_1247 at 1403.118 kb on + strand, within Psyr_1247 at 1403.270 kb on + strand at 1403.270 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.331 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine 10 mM remove 1,401,390 + Psyr_1245 0.86 +1.2 1,401,487 + +1.9 1,401,487 + -1.4 1,401,487 + +0.7 1,401,487 + +1.2 1,401,487 + -2.1 1,401,487 + -0.5 1,401,487 + -2.7 1,401,487 + -2.3 1,401,487 + +0.7 1,401,488 - +0.8 1,401,489 + -0.7 1,401,489 + +0.0 1,401,489 + +1.6 1,401,490 - -0.9 1,401,491 + +1.4 1,401,491 + +2.0 1,401,491 + +0.1 1,401,491 + +0.4 1,401,491 + -0.7 1,401,491 + +1.8 1,401,492 - +1.3 1,401,492 - +1.1 1,401,696 + Psyr_1246 0.15 -0.0 1,401,696 + Psyr_1246 0.15 +1.3 1,401,696 + Psyr_1246 0.15 -0.7 1,401,909 + Psyr_1246 0.43 +0.3 1,401,939 + Psyr_1246 0.47 +0.6 1,402,149 + Psyr_1246 0.75 +0.6 1,402,149 + Psyr_1246 0.75 -0.2 1,402,149 + Psyr_1246 0.75 +3.3 1,402,149 + Psyr_1246 0.75 -3.1 1,402,149 + Psyr_1246 0.75 -0.8 1,402,149 + Psyr_1246 0.75 -1.1 1,402,149 + Psyr_1246 0.75 +1.5 1,402,167 + Psyr_1246 0.77 +0.8 1,402,170 + Psyr_1246 0.78 -2.8 1,402,208 + Psyr_1246 0.83 -0.7 1,402,208 + Psyr_1246 0.83 +2.3 1,402,208 + Psyr_1246 0.83 -1.2 1,402,213 + Psyr_1246 0.84 +0.3 1,402,240 + Psyr_1246 0.87 -0.3 1,402,240 + Psyr_1246 0.87 +1.1 1,402,240 + Psyr_1246 0.87 -0.8 1,402,240 + Psyr_1246 0.87 -0.3 1,402,240 + Psyr_1246 0.87 +0.6 1,402,240 + Psyr_1246 0.87 +0.1 1,402,240 + Psyr_1246 0.87 -0.5 1,402,242 + Psyr_1246 0.87 -1.0 1,402,242 + Psyr_1246 0.87 -0.2 1,402,242 + Psyr_1246 0.87 +1.0 1,402,242 + Psyr_1246 0.87 -1.9 1,402,242 + Psyr_1246 0.87 -0.2 1,402,242 + Psyr_1246 0.87 +0.9 1,402,242 + Psyr_1246 0.87 +0.7 1,402,242 + Psyr_1246 0.87 -0.1 1,402,242 + Psyr_1246 0.87 +0.2 1,402,242 + Psyr_1246 0.87 +1.5 1,402,242 + Psyr_1246 0.87 +0.7 1,402,242 + Psyr_1246 0.87 -0.9 1,402,242 + Psyr_1246 0.87 +1.2 1,402,242 + Psyr_1246 0.87 +0.0 1,402,242 + Psyr_1246 0.87 -0.2 1,402,242 + Psyr_1246 0.87 +1.2 1,402,242 + Psyr_1246 0.87 -0.3 1,402,311 + +0.2 1,402,320 + +0.5 1,402,320 + -0.8 1,402,320 + +1.5 1,402,320 + -0.5 1,402,320 + +2.9 1,402,513 + Psyr_1247 0.18 -1.3 1,402,513 + Psyr_1247 0.18 -1.8 1,402,529 + Psyr_1247 0.19 -2.7 1,402,529 + Psyr_1247 0.19 -1.5 1,402,857 + Psyr_1247 0.52 +0.4 1,403,118 + Psyr_1247 0.78 +1.4 1,403,270 + -1.1 1,403,270 + -1.7 1,403,292 + -1.5 1,403,292 + -0.8 1,403,292 + -0.3 1,403,292 + +2.4 1,403,292 + -1.2 1,403,292 + +0.1 1,403,292 + +1.5 1,403,331 + -0.6
Or see this region's nucleotide sequence