Experiment: KB with Palmatine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0664 and Psyr_0665 are separated by 4 nucleotides Psyr_0665 and Psyr_0666 overlap by 4 nucleotides Psyr_0666 and Psyr_0667 are separated by 0 nucleotides
Psyr_0664: Psyr_0664 - ABC transporter, at 752,808 to 753,599
_0664
Psyr_0665: Psyr_0665 - transport system permease protein, at 753,604 to 754,572
_0665
Psyr_0666: Psyr_0666 - transport system permease protein, at 754,569 to 755,558
_0666
Psyr_0667: Psyr_0667 - Periplasmic binding protein, at 755,559 to 756,479
_0667
Position (kb) 753
754
755 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 752.607 kb on + strand at 752.607 kb on + strand at 752.607 kb on + strand at 752.608 kb on - strand at 752.608 kb on - strand at 752.632 kb on + strand at 752.632 kb on + strand at 752.632 kb on + strand at 752.639 kb on - strand at 752.639 kb on - strand at 752.678 kb on + strand at 752.679 kb on - strand at 752.679 kb on - strand at 752.679 kb on - strand at 752.761 kb on + strand at 752.761 kb on + strand at 752.761 kb on + strand at 752.762 kb on - strand at 752.886 kb on + strand at 752.886 kb on + strand at 752.886 kb on + strand at 752.886 kb on + strand at 752.887 kb on - strand at 752.887 kb on - strand at 752.887 kb on - strand at 753.094 kb on + strand, within Psyr_0664 at 753.094 kb on + strand, within Psyr_0664 at 753.095 kb on - strand, within Psyr_0664 at 753.095 kb on - strand, within Psyr_0664 at 753.095 kb on - strand, within Psyr_0664 at 753.593 kb on + strand at 753.594 kb on - strand at 753.594 kb on - strand at 753.594 kb on - strand at 753.765 kb on - strand, within Psyr_0665 at 753.765 kb on - strand, within Psyr_0665 at 753.895 kb on - strand, within Psyr_0665 at 754.181 kb on + strand, within Psyr_0665 at 754.181 kb on + strand, within Psyr_0665 at 754.181 kb on + strand, within Psyr_0665 at 754.181 kb on + strand, within Psyr_0665 at 754.181 kb on + strand, within Psyr_0665 at 754.181 kb on + strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.182 kb on - strand, within Psyr_0665 at 754.914 kb on - strand, within Psyr_0666 at 755.056 kb on - strand, within Psyr_0666 at 755.407 kb on + strand, within Psyr_0666 at 755.407 kb on + strand, within Psyr_0666 at 755.407 kb on + strand, within Psyr_0666 at 755.408 kb on - strand, within Psyr_0666 at 755.408 kb on - strand, within Psyr_0666 at 755.560 kb on + strand at 755.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine 10 mM remove 752,607 + -2.0 752,607 + -1.0 752,607 + -0.1 752,608 - +0.2 752,608 - +1.8 752,632 + +2.2 752,632 + -0.1 752,632 + -0.9 752,639 - +0.8 752,639 - -0.3 752,678 + +4.2 752,679 - +1.2 752,679 - -1.2 752,679 - -2.7 752,761 + -2.0 752,761 + -0.2 752,761 + -2.5 752,762 - +1.2 752,886 + +0.7 752,886 + -1.0 752,886 + +1.2 752,886 + -0.3 752,887 - -2.1 752,887 - -1.6 752,887 - -0.6 753,094 + Psyr_0664 0.36 +0.1 753,094 + Psyr_0664 0.36 -2.3 753,095 - Psyr_0664 0.36 -0.4 753,095 - Psyr_0664 0.36 -1.9 753,095 - Psyr_0664 0.36 +1.7 753,593 + -1.3 753,594 - -1.8 753,594 - -1.7 753,594 - +1.2 753,765 - Psyr_0665 0.17 +3.6 753,765 - Psyr_0665 0.17 +1.2 753,895 - Psyr_0665 0.30 -0.1 754,181 + Psyr_0665 0.60 -0.8 754,181 + Psyr_0665 0.60 +0.2 754,181 + Psyr_0665 0.60 +0.4 754,181 + Psyr_0665 0.60 -0.8 754,181 + Psyr_0665 0.60 +0.2 754,181 + Psyr_0665 0.60 +0.5 754,182 - Psyr_0665 0.60 -0.5 754,182 - Psyr_0665 0.60 -1.5 754,182 - Psyr_0665 0.60 +1.5 754,182 - Psyr_0665 0.60 +0.8 754,182 - Psyr_0665 0.60 -1.8 754,182 - Psyr_0665 0.60 -0.5 754,182 - Psyr_0665 0.60 +1.5 754,182 - Psyr_0665 0.60 -0.1 754,182 - Psyr_0665 0.60 +0.1 754,182 - Psyr_0665 0.60 -2.3 754,182 - Psyr_0665 0.60 +1.7 754,182 - Psyr_0665 0.60 -2.0 754,182 - Psyr_0665 0.60 +1.7 754,914 - Psyr_0666 0.35 -3.4 755,056 - Psyr_0666 0.49 -0.9 755,407 + Psyr_0666 0.85 -1.6 755,407 + Psyr_0666 0.85 +0.9 755,407 + Psyr_0666 0.85 +1.0 755,408 - Psyr_0666 0.85 +2.2 755,408 - Psyr_0666 0.85 -0.1 755,560 + -2.8 755,561 - +1.8
Or see this region's nucleotide sequence