Experiment: KB with Palmatine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0270 and Psyr_0271 are separated by 74 nucleotides Psyr_0271 and Psyr_0272 are separated by 36 nucleotides Psyr_0272 and Psyr_0273 are separated by 94 nucleotides Psyr_0273 and Psyr_0274 overlap by 1 nucleotides
Psyr_0270: Psyr_0270 - DNA polymerase I, at 287,690 to 290,455
_0270
Psyr_0271: Psyr_0271 - conserved hypothetical protein, at 290,530 to 290,817
_0271
Psyr_0272: Psyr_0272 - homoserine kinase, at 290,854 to 291,804
_0272
Psyr_0273: Psyr_0273 - Integrase, catalytic region, at 291,899 to 292,735
_0273
Psyr_0274: Psyr_0274 - ISPsy9, transposase OrfA, at 292,735 to 293,274
_0274
Position (kb) 290
291
292 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 290.540 kb on + strand at 290.552 kb on + strand at 290.552 kb on + strand at 290.552 kb on + strand at 290.628 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.686 kb on + strand, within Psyr_0271 at 290.687 kb on - strand, within Psyr_0271 at 290.722 kb on + strand, within Psyr_0271 at 290.745 kb on + strand, within Psyr_0271 at 290.745 kb on + strand, within Psyr_0271 at 290.804 kb on + strand at 290.804 kb on + strand at 290.804 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 291.781 kb on - strand at 291.833 kb on + strand at 291.833 kb on + strand at 291.833 kb on + strand at 291.833 kb on + strand at 291.834 kb on - strand at 291.893 kb on - strand at 291.901 kb on - strand at 291.901 kb on - strand at 291.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine 10 mM remove 290,540 + +2.5 290,552 + +0.3 290,552 + -1.0 290,552 + +0.3 290,628 + Psyr_0271 0.34 -0.6 290,631 + Psyr_0271 0.35 -1.4 290,631 + Psyr_0271 0.35 -1.2 290,686 + Psyr_0271 0.54 -0.7 290,687 - Psyr_0271 0.55 -0.9 290,722 + Psyr_0271 0.67 -0.1 290,745 + Psyr_0271 0.75 -0.5 290,745 + Psyr_0271 0.75 +0.4 290,804 + +0.8 290,804 + -0.1 290,804 + +0.1 290,823 + +1.6 290,823 + -1.6 290,823 + -0.2 290,823 + -3.7 290,823 + -0.7 291,781 - +2.0 291,833 + +0.8 291,833 + -0.7 291,833 + +0.4 291,833 + +0.1 291,834 - +1.9 291,893 - -0.5 291,901 - +2.0 291,901 - -1.3 291,901 - -0.7
Or see this region's nucleotide sequence