Experiment: KB with Palmatine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0201 and Psyr_0202 are separated by 11 nucleotides Psyr_0202 and Psyr_0203 are separated by 92 nucleotides Psyr_0203 and Psyr_0204 overlap by 4 nucleotides
Psyr_0201: Psyr_0201 - ATP-dependent DNA helicase RecG, at 215,460 to 217,535
_0201
Psyr_0202: Psyr_0202 - transcriptional regulator, LysR family, at 217,547 to 218,470
_0202
Psyr_0203: Psyr_0203 - outer membrane transport energization protein TonB, at 218,563 to 219,309
_0203
Psyr_0204: Psyr_0204 - outer membrane transport energization protein ExbD, at 219,306 to 219,731
_0204
Position (kb) 217
218
219 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 216.643 kb on - strand, within Psyr_0201 at 216.692 kb on - strand, within Psyr_0201 at 217.087 kb on + strand, within Psyr_0201 at 217.134 kb on - strand, within Psyr_0201 at 217.156 kb on + strand, within Psyr_0201 at 217.156 kb on + strand, within Psyr_0201 at 217.156 kb on + strand, within Psyr_0201 at 217.156 kb on + strand, within Psyr_0201 at 217.157 kb on - strand, within Psyr_0201 at 217.157 kb on - strand, within Psyr_0201 at 217.157 kb on - strand, within Psyr_0201 at 217.157 kb on - strand, within Psyr_0201 at 217.252 kb on + strand, within Psyr_0201 at 217.253 kb on - strand, within Psyr_0201 at 217.548 kb on + strand at 217.548 kb on + strand at 217.548 kb on + strand at 217.548 kb on + strand at 217.802 kb on + strand, within Psyr_0202 at 217.802 kb on + strand, within Psyr_0202 at 217.802 kb on + strand, within Psyr_0202 at 217.802 kb on + strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 218.103 kb on + strand, within Psyr_0202 at 218.103 kb on + strand, within Psyr_0202 at 218.103 kb on + strand, within Psyr_0202 at 218.103 kb on + strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.161 kb on - strand, within Psyr_0202 at 218.175 kb on + strand, within Psyr_0202 at 218.176 kb on - strand, within Psyr_0202 at 218.176 kb on - strand, within Psyr_0202 at 218.448 kb on + strand at 218.448 kb on + strand at 218.448 kb on + strand at 218.449 kb on - strand at 218.461 kb on + strand at 218.461 kb on + strand at 218.461 kb on + strand at 218.462 kb on - strand at 218.470 kb on + strand at 218.471 kb on - strand at 218.471 kb on - strand at 218.538 kb on + strand at 218.538 kb on + strand at 218.539 kb on - strand at 218.543 kb on - strand at 218.557 kb on + strand at 219.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Palmatine 10 mM remove 216,643 - Psyr_0201 0.57 +1.0 216,692 - Psyr_0201 0.59 -1.9 217,087 + Psyr_0201 0.78 -2.2 217,134 - Psyr_0201 0.81 -1.9 217,156 + Psyr_0201 0.82 -1.1 217,156 + Psyr_0201 0.82 -2.3 217,156 + Psyr_0201 0.82 -1.7 217,156 + Psyr_0201 0.82 +0.4 217,157 - Psyr_0201 0.82 -2.0 217,157 - Psyr_0201 0.82 -3.2 217,157 - Psyr_0201 0.82 +2.6 217,157 - Psyr_0201 0.82 -2.0 217,252 + Psyr_0201 0.86 -3.1 217,253 - Psyr_0201 0.86 -2.3 217,548 + -0.3 217,548 + -1.1 217,548 + -0.3 217,548 + -0.3 217,802 + Psyr_0202 0.28 -0.7 217,802 + Psyr_0202 0.28 -0.5 217,802 + Psyr_0202 0.28 -1.5 217,802 + Psyr_0202 0.28 -0.1 217,803 - Psyr_0202 0.28 -2.6 217,803 - Psyr_0202 0.28 -1.3 217,803 - Psyr_0202 0.28 -1.1 217,803 - Psyr_0202 0.28 -3.0 217,803 - Psyr_0202 0.28 -3.4 218,103 + Psyr_0202 0.60 -0.5 218,103 + Psyr_0202 0.60 -1.0 218,103 + Psyr_0202 0.60 -2.0 218,103 + Psyr_0202 0.60 -2.0 218,104 - Psyr_0202 0.60 -2.5 218,104 - Psyr_0202 0.60 -2.0 218,104 - Psyr_0202 0.60 -0.7 218,104 - Psyr_0202 0.60 -2.0 218,161 - Psyr_0202 0.66 -1.8 218,175 + Psyr_0202 0.68 -2.3 218,176 - Psyr_0202 0.68 -0.7 218,176 - Psyr_0202 0.68 -1.3 218,448 + -2.5 218,448 + -2.4 218,448 + -3.5 218,449 - -1.0 218,461 + -1.4 218,461 + -1.6 218,461 + -2.3 218,462 - -1.0 218,470 + -2.1 218,471 - -0.5 218,471 - +1.2 218,538 + +0.8 218,538 + -1.0 218,539 - -0.9 218,543 - +0.8 218,557 + +0.7 219,334 - -0.4
Or see this region's nucleotide sequence