Experiment: KB with Levodopa 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0165 and Psyr_0166 are separated by 563 nucleotides Psyr_0166 and Psyr_0167 are separated by 5 nucleotides Psyr_0167 and Psyr_0168 are separated by 370 nucleotides
Psyr_0165: Psyr_0165 - GCN5-related N-acetyltransferase, at 179,100 to 179,543
_0165
Psyr_0166: Psyr_0166 - hypothetical protein, at 180,107 to 181,417
_0166
Psyr_0167: Psyr_0167 - hypothetical protein, at 181,423 to 181,845
_0167
Psyr_0168: Psyr_0168 - Phosphoenolpyruvate carboxykinase (ATP), at 182,216 to 183,760
_0168
Position (kb)
180
181
182 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.424 kb on + strand, within Psyr_0165 at 179.550 kb on + strand at 179.550 kb on + strand at 179.550 kb on + strand at 179.551 kb on - strand at 179.551 kb on - strand at 179.644 kb on + strand at 179.732 kb on + strand at 179.769 kb on + strand at 179.770 kb on - strand at 179.855 kb on + strand at 179.856 kb on - strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.866 kb on + strand at 179.866 kb on + strand at 179.867 kb on - strand at 179.867 kb on - strand at 179.878 kb on - strand at 179.927 kb on - strand at 180.052 kb on + strand at 180.065 kb on + strand at 180.065 kb on + strand at 180.066 kb on - strand at 180.076 kb on + strand at 180.140 kb on + strand at 180.141 kb on - strand at 180.195 kb on + strand at 180.195 kb on + strand at 180.195 kb on + strand at 180.196 kb on - strand at 180.217 kb on + strand at 180.524 kb on - strand, within Psyr_0166 at 180.530 kb on - strand, within Psyr_0166 at 180.530 kb on - strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.585 kb on + strand, within Psyr_0166 at 180.650 kb on + strand, within Psyr_0166 at 180.813 kb on + strand, within Psyr_0166 at 180.878 kb on - strand, within Psyr_0166 at 180.985 kb on + strand, within Psyr_0166 at 180.986 kb on - strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 181.013 kb on - strand, within Psyr_0166 at 181.016 kb on + strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.053 kb on - strand, within Psyr_0166 at 181.053 kb on - strand, within Psyr_0166 at 181.091 kb on + strand, within Psyr_0166 at 181.150 kb on + strand, within Psyr_0166 at 181.184 kb on + strand, within Psyr_0166 at 181.185 kb on - strand, within Psyr_0166 at 181.185 kb on - strand, within Psyr_0166 at 181.236 kb on + strand, within Psyr_0166 at 181.274 kb on + strand, within Psyr_0166 at 181.286 kb on + strand, within Psyr_0166 at 181.287 kb on - strand at 181.287 kb on - strand at 181.287 kb on - strand at 181.288 kb on + strand at 181.288 kb on + strand at 181.375 kb on + strand at 181.377 kb on + strand at 181.378 kb on - strand at 181.542 kb on - strand, within Psyr_0167 at 181.542 kb on - strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.695 kb on + strand, within Psyr_0167 at 181.696 kb on - strand, within Psyr_0167 at 181.753 kb on - strand, within Psyr_0167 at 181.756 kb on - strand, within Psyr_0167 at 181.771 kb on - strand, within Psyr_0167 at 181.847 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.848 kb on - strand at 181.900 kb on + strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.905 kb on - strand at 181.905 kb on - strand at 181.906 kb on + strand at 181.907 kb on - strand at 181.913 kb on + strand at 181.914 kb on - strand at 182.054 kb on - strand at 182.086 kb on + strand at 182.086 kb on + strand at 182.086 kb on + strand at 182.086 kb on + strand at 182.086 kb on + strand at 182.087 kb on - strand at 182.087 kb on - strand at 182.087 kb on - strand at 182.103 kb on + strand at 182.207 kb on + strand at 182.207 kb on + strand at 182.207 kb on + strand at 182.207 kb on + strand at 182.208 kb on - strand at 182.208 kb on - strand at 182.208 kb on - strand at 182.217 kb on + strand at 182.217 kb on + strand at 182.218 kb on - strand at 182.314 kb on - strand at 182.384 kb on + strand, within Psyr_0168
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Levodopa 10 mM remove 179,128 + +0.3 179,128 + -0.2 179,128 + -0.8 179,128 + -1.3 179,128 + -1.1 179,128 + -1.5 179,128 + +0.4 179,128 + +0.8 179,128 + -0.5 179,128 + -0.1 179,128 + -0.3 179,128 + -3.3 179,128 + +0.3 179,128 + -2.1 179,128 + -0.4 179,129 - +0.3 179,129 - +0.5 179,129 - +1.0 179,129 - -1.7 179,129 - +1.7 179,129 - -0.9 179,129 - -1.3 179,129 - +0.3 179,129 - -0.5 179,253 + Psyr_0165 0.34 -0.1 179,253 + Psyr_0165 0.34 -0.6 179,253 + Psyr_0165 0.34 -3.2 179,253 + Psyr_0165 0.34 -0.7 179,253 + Psyr_0165 0.34 -0.3 179,254 - Psyr_0165 0.35 +0.4 179,254 - Psyr_0165 0.35 -1.2 179,254 - Psyr_0165 0.35 -0.8 179,254 - Psyr_0165 0.35 -1.1 179,254 - Psyr_0165 0.35 -0.7 179,424 + Psyr_0165 0.73 -1.3 179,550 + +1.3 179,550 + +0.5 179,550 + +0.3 179,551 - -1.1 179,551 - +1.6 179,644 + +0.3 179,732 + -2.7 179,769 + -1.0 179,770 - +0.0 179,855 + +0.1 179,856 - -0.9 179,862 + -0.9 179,862 + +1.3 179,862 + +0.8 179,866 + -2.3 179,866 + +0.7 179,867 - -1.9 179,867 - -1.5 179,878 - -1.5 179,927 - +2.8 180,052 + +0.2 180,065 + +0.0 180,065 + -0.3 180,066 - -0.6 180,076 + -0.1 180,140 + -0.3 180,141 - +1.3 180,195 + -0.9 180,195 + -2.2 180,195 + +0.2 180,196 - -0.6 180,217 + +0.5 180,524 - Psyr_0166 0.32 -1.6 180,530 - Psyr_0166 0.32 -1.6 180,530 - Psyr_0166 0.32 -2.2 180,533 + Psyr_0166 0.32 -0.4 180,533 + Psyr_0166 0.32 +0.0 180,533 + Psyr_0166 0.32 +2.6 180,534 - Psyr_0166 0.33 -0.7 180,534 - Psyr_0166 0.33 +1.3 180,534 - Psyr_0166 0.33 +0.6 180,534 - Psyr_0166 0.33 -0.1 180,534 - Psyr_0166 0.33 -2.0 180,585 + Psyr_0166 0.36 -4.0 180,650 + Psyr_0166 0.41 +3.4 180,813 + Psyr_0166 0.54 -0.4 180,878 - Psyr_0166 0.59 -2.5 180,985 + Psyr_0166 0.67 +1.0 180,986 - Psyr_0166 0.67 -0.4 180,995 + Psyr_0166 0.68 -1.1 180,996 - Psyr_0166 0.68 -1.9 180,996 - Psyr_0166 0.68 -1.3 181,013 - Psyr_0166 0.69 +2.1 181,016 + Psyr_0166 0.69 +1.0 181,042 + Psyr_0166 0.71 -0.7 181,053 - Psyr_0166 0.72 -0.8 181,053 - Psyr_0166 0.72 -1.3 181,091 + Psyr_0166 0.75 +0.9 181,150 + Psyr_0166 0.80 -0.6 181,184 + Psyr_0166 0.82 +1.2 181,185 - Psyr_0166 0.82 +1.6 181,185 - Psyr_0166 0.82 +2.4 181,236 + Psyr_0166 0.86 -2.7 181,274 + Psyr_0166 0.89 +1.9 181,286 + Psyr_0166 0.90 +0.4 181,287 - -0.1 181,287 - -1.5 181,287 - -0.6 181,288 + -1.0 181,288 + -0.6 181,375 + -0.2 181,377 + +1.0 181,378 - -1.5 181,542 - Psyr_0167 0.28 +0.5 181,542 - Psyr_0167 0.28 -2.4 181,693 + Psyr_0167 0.64 -0.7 181,694 - Psyr_0167 0.64 +0.9 181,695 + Psyr_0167 0.64 +1.1 181,696 - Psyr_0167 0.65 -1.5 181,753 - Psyr_0167 0.78 -1.0 181,756 - Psyr_0167 0.79 -0.7 181,771 - Psyr_0167 0.82 -0.2 181,847 + +0.4 181,847 + -0.6 181,847 + +0.3 181,848 - +0.5 181,900 + -2.6 181,904 + -0.8 181,904 + +0.5 181,905 - -2.0 181,905 - -1.1 181,906 + -1.5 181,907 - -0.5 181,913 + -2.3 181,914 - -0.1 182,054 - +0.4 182,086 + -1.1 182,086 + +1.4 182,086 + +0.9 182,086 + -1.8 182,086 + -1.2 182,087 - -0.1 182,087 - +0.4 182,087 - +1.7 182,103 + +0.2 182,207 + -0.7 182,207 + +0.6 182,207 + +0.8 182,207 + -1.5 182,208 - +2.1 182,208 - -1.3 182,208 - -0.6 182,217 + +0.3 182,217 + -0.5 182,218 - -0.8 182,314 - -0.3 182,384 + Psyr_0168 0.11 -0.1
Or see this region's nucleotide sequence