Experiment: KB with Piperic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2465 and Psyr_2466 are separated by 70 nucleotides Psyr_2466 and Psyr_2467 are separated by 96 nucleotides Psyr_2467 and Psyr_2468 overlap by 4 nucleotides Psyr_2468 and Psyr_2469 are separated by 14 nucleotides
Psyr_2465: Psyr_2465 - Acyltransferase 3, at 2,857,032 to 2,858,054
_2465
Psyr_2466: Psyr_2466 - HesB/YadR/YfhF:Nitrogen-fixing NifU, C-terminal, at 2,858,125 to 2,858,718
_2466
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Psyr_2468: Psyr_2468 - Enoyl-CoA hydratase/isomerase, at 2,860,761 to 2,861,636
_2468
Psyr_2469: Psyr_2469 - Propionyl-CoA carboxylase, at 2,861,651 to 2,863,258
_2469
Position (kb)
2858
2859
2860
2861 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2857.836 kb on + strand, within Psyr_2465 at 2857.837 kb on - strand, within Psyr_2465 at 2857.845 kb on + strand, within Psyr_2465 at 2857.846 kb on - strand, within Psyr_2465 at 2857.869 kb on + strand, within Psyr_2465 at 2858.062 kb on - strand at 2858.710 kb on + strand at 2858.711 kb on - strand at 2858.711 kb on - strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.714 kb on - strand at 2858.716 kb on + strand at 2858.717 kb on - strand at 2859.105 kb on - strand, within Psyr_2467 at 2859.105 kb on - strand, within Psyr_2467 at 2859.112 kb on - strand, within Psyr_2467 at 2859.191 kb on - strand, within Psyr_2467 at 2859.605 kb on + strand, within Psyr_2467 at 2859.680 kb on + strand, within Psyr_2467 at 2859.849 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.149 kb on - strand, within Psyr_2467 at 2860.352 kb on + strand, within Psyr_2467 at 2860.575 kb on - strand at 2860.675 kb on - strand at 2861.140 kb on + strand, within Psyr_2468 at 2861.348 kb on - strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.392 kb on + strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.393 kb on - strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.496 kb on + strand, within Psyr_2468 at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.610 kb on + strand at 2861.677 kb on + strand at 2861.677 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Piperic 10 mM remove 2,857,836 + Psyr_2465 0.79 -0.6 2,857,837 - Psyr_2465 0.79 +0.1 2,857,845 + Psyr_2465 0.79 -2.1 2,857,846 - Psyr_2465 0.80 +0.3 2,857,869 + Psyr_2465 0.82 -0.2 2,858,062 - +0.3 2,858,710 + +3.1 2,858,711 - +1.3 2,858,711 - +1.0 2,858,713 + +1.3 2,858,713 + -1.1 2,858,713 + -0.6 2,858,714 - +0.0 2,858,716 + +0.7 2,858,717 - +0.3 2,859,105 - Psyr_2467 0.15 -0.7 2,859,105 - Psyr_2467 0.15 +1.0 2,859,112 - Psyr_2467 0.15 +0.5 2,859,191 - Psyr_2467 0.19 +1.5 2,859,605 + Psyr_2467 0.41 +0.0 2,859,680 + Psyr_2467 0.44 +2.3 2,859,849 - Psyr_2467 0.53 -0.1 2,860,149 - Psyr_2467 0.68 -0.7 2,860,149 - Psyr_2467 0.68 -0.5 2,860,149 - Psyr_2467 0.68 +0.4 2,860,352 + Psyr_2467 0.79 +0.3 2,860,575 - +1.5 2,860,675 - +0.8 2,861,140 + Psyr_2468 0.43 +1.3 2,861,348 - Psyr_2468 0.67 +0.2 2,861,392 + Psyr_2468 0.72 +1.1 2,861,392 + Psyr_2468 0.72 +3.6 2,861,392 + Psyr_2468 0.72 +0.6 2,861,393 - Psyr_2468 0.72 +1.5 2,861,393 - Psyr_2468 0.72 +0.9 2,861,496 + Psyr_2468 0.84 -2.7 2,861,496 + Psyr_2468 0.84 +1.3 2,861,496 + Psyr_2468 0.84 +1.0 2,861,610 + +1.7 2,861,610 + +1.3 2,861,610 + +0.3 2,861,677 + -1.9 2,861,677 + -0.7
Or see this region's nucleotide sequence