Experiment: KB with Piperic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0697 and Psyr_0698 are separated by 33 nucleotides Psyr_0698 and Psyr_0699 are separated by 6 nucleotides Psyr_0699 and Psyr_0700 are separated by 146 nucleotides
Psyr_0697: Psyr_0697 - tRNA 2-selenouridine synthase, at 801,532 to 802,632
_0697
Psyr_0698: Psyr_0698 - CDS, at 802,666 to 802,965
_0698
Psyr_0699: Psyr_0699 - CDS, at 802,972 to 803,430
_0699
Psyr_0700: Psyr_0700 - Trans-hexaprenyltranstransferase, at 803,577 to 804,545
_0700
Position (kb) 802
803
804 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 802.001 kb on + strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.002 kb on - strand, within Psyr_0697 at 802.294 kb on + strand, within Psyr_0697 at 802.294 kb on + strand, within Psyr_0697 at 802.294 kb on + strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.295 kb on - strand, within Psyr_0697 at 802.389 kb on + strand, within Psyr_0697 at 802.389 kb on + strand, within Psyr_0697 at 802.390 kb on - strand, within Psyr_0697 at 802.519 kb on + strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.520 kb on - strand, within Psyr_0697 at 802.523 kb on - strand at 802.523 kb on - strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.534 kb on + strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.535 kb on - strand at 802.687 kb on + strand at 802.688 kb on - strand at 802.688 kb on - strand at 803.158 kb on + strand, within Psyr_0699 at 803.158 kb on + strand, within Psyr_0699 at 803.158 kb on + strand, within Psyr_0699 at 803.159 kb on - strand, within Psyr_0699 at 803.159 kb on - strand, within Psyr_0699 at 803.159 kb on - strand, within Psyr_0699 at 803.421 kb on - strand at 803.421 kb on - strand at 803.421 kb on - strand at 803.421 kb on - strand at 803.445 kb on + strand at 803.445 kb on + strand at 803.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Piperic 10 mM remove 802,001 + Psyr_0697 0.43 -0.2 802,002 - Psyr_0697 0.43 +0.9 802,002 - Psyr_0697 0.43 -0.4 802,002 - Psyr_0697 0.43 +1.6 802,002 - Psyr_0697 0.43 +1.4 802,002 - Psyr_0697 0.43 -0.9 802,002 - Psyr_0697 0.43 -2.0 802,294 + Psyr_0697 0.69 +0.5 802,294 + Psyr_0697 0.69 +2.8 802,294 + Psyr_0697 0.69 -0.8 802,295 - Psyr_0697 0.69 -0.9 802,295 - Psyr_0697 0.69 +0.4 802,295 - Psyr_0697 0.69 -0.3 802,295 - Psyr_0697 0.69 +1.4 802,295 - Psyr_0697 0.69 +0.0 802,295 - Psyr_0697 0.69 -0.0 802,295 - Psyr_0697 0.69 -0.8 802,295 - Psyr_0697 0.69 +0.1 802,389 + Psyr_0697 0.78 +0.4 802,389 + Psyr_0697 0.78 -0.4 802,390 - Psyr_0697 0.78 -0.9 802,519 + Psyr_0697 0.90 -2.1 802,520 - Psyr_0697 0.90 -0.1 802,520 - Psyr_0697 0.90 -0.2 802,520 - Psyr_0697 0.90 -0.9 802,520 - Psyr_0697 0.90 -1.0 802,523 - -0.3 802,523 - +0.2 802,534 + +0.1 802,534 + -0.5 802,534 + +0.5 802,534 + +1.1 802,534 + -0.6 802,534 + -0.4 802,534 + +0.9 802,534 + +0.7 802,535 - -0.9 802,535 - -2.2 802,535 - +0.6 802,535 - +0.3 802,535 - +0.1 802,535 - -0.5 802,535 - +1.0 802,687 + +0.6 802,688 - +1.6 802,688 - -0.9 803,158 + Psyr_0699 0.41 +0.2 803,158 + Psyr_0699 0.41 +0.6 803,158 + Psyr_0699 0.41 +1.8 803,159 - Psyr_0699 0.41 -0.3 803,159 - Psyr_0699 0.41 +4.2 803,159 - Psyr_0699 0.41 +1.1 803,421 - -0.8 803,421 - +3.2 803,421 - -0.1 803,421 - +1.4 803,445 + -1.0 803,445 + -0.3 803,517 + -1.2
Or see this region's nucleotide sequence