Experiment: KB with Piperic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0310 and Psyr_0311 are separated by 98 nucleotides Psyr_0311 and Psyr_0312 are separated by 119 nucleotides Psyr_0312 and Psyr_0313 are separated by 255 nucleotides
Psyr_0310: Psyr_0310 - HNH endonuclease:S-type Pyocin, at 329,734 to 331,704
_0310
Psyr_0311: Psyr_0311 - CDS, at 331,803 to 332,150
_0311
Psyr_0312: Psyr_0312 - hypothetical protein, at 332,270 to 332,632
_0312
Psyr_0313: Psyr_0313 - Zinc-containing alcohol dehydrogenase superfamily, at 332,888 to 333,874
_0313
Position (kb) 332
333 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 331.399 kb on + strand, within Psyr_0310 at 331.399 kb on + strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.400 kb on - strand, within Psyr_0310 at 331.477 kb on + strand, within Psyr_0310 at 331.494 kb on + strand, within Psyr_0310 at 331.494 kb on + strand, within Psyr_0310 at 331.498 kb on - strand, within Psyr_0310 at 331.503 kb on + strand, within Psyr_0310 at 331.503 kb on + strand, within Psyr_0310 at 331.504 kb on - strand, within Psyr_0310 at 331.531 kb on + strand at 331.590 kb on + strand at 331.590 kb on + strand at 331.590 kb on + strand at 331.591 kb on - strand at 331.591 kb on - strand at 331.608 kb on + strand at 331.609 kb on - strand at 331.648 kb on + strand at 331.673 kb on - strand at 331.676 kb on + strand at 331.676 kb on + strand at 331.676 kb on + strand at 331.677 kb on - strand at 331.677 kb on - strand at 331.677 kb on - strand at 331.677 kb on - strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.678 kb on + strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.679 kb on - strand at 331.681 kb on - strand at 331.717 kb on - strand at 331.719 kb on + strand at 331.725 kb on + strand at 331.734 kb on + strand at 331.766 kb on - strand at 331.769 kb on + strand at 331.787 kb on + strand at 331.788 kb on - strand at 331.788 kb on - strand at 331.804 kb on + strand at 331.804 kb on + strand at 331.804 kb on + strand at 332.144 kb on - strand at 332.144 kb on - strand at 332.230 kb on + strand at 332.230 kb on + strand at 332.230 kb on + strand at 332.230 kb on + strand at 332.231 kb on - strand at 332.246 kb on - strand at 332.337 kb on + strand, within Psyr_0312 at 332.337 kb on + strand, within Psyr_0312 at 332.338 kb on - strand, within Psyr_0312 at 332.338 kb on - strand, within Psyr_0312 at 332.355 kb on - strand, within Psyr_0312 at 332.355 kb on - strand, within Psyr_0312 at 332.356 kb on + strand, within Psyr_0312 at 332.425 kb on + strand, within Psyr_0312 at 332.425 kb on + strand, within Psyr_0312 at 332.448 kb on - strand, within Psyr_0312 at 332.451 kb on + strand, within Psyr_0312 at 332.451 kb on + strand, within Psyr_0312 at 332.451 kb on + strand, within Psyr_0312 at 332.451 kb on + strand, within Psyr_0312 at 332.452 kb on - strand, within Psyr_0312 at 332.475 kb on - strand, within Psyr_0312 at 332.475 kb on - strand, within Psyr_0312 at 332.488 kb on + strand, within Psyr_0312 at 332.496 kb on - strand, within Psyr_0312 at 332.523 kb on - strand, within Psyr_0312 at 332.550 kb on + strand, within Psyr_0312 at 332.564 kb on + strand, within Psyr_0312 at 332.593 kb on - strand, within Psyr_0312 at 332.593 kb on - strand, within Psyr_0312 at 332.593 kb on - strand, within Psyr_0312 at 332.637 kb on + strand at 332.637 kb on + strand at 332.645 kb on - strand at 332.648 kb on - strand at 332.660 kb on - strand at 332.709 kb on + strand at 332.712 kb on + strand at 332.712 kb on + strand at 332.713 kb on - strand at 332.713 kb on - strand at 332.713 kb on - strand at 332.713 kb on - strand at 332.713 kb on - strand at 332.713 kb on - strand at 332.716 kb on + strand at 332.716 kb on + strand at 332.717 kb on - strand at 332.726 kb on + strand at 332.740 kb on - strand at 332.772 kb on + strand at 332.772 kb on + strand at 332.772 kb on + strand at 332.772 kb on + strand at 332.772 kb on + strand at 332.773 kb on - strand at 332.773 kb on - strand at 332.844 kb on + strand at 332.878 kb on + strand at 332.878 kb on + strand at 332.878 kb on + strand at 332.879 kb on - strand at 332.879 kb on - strand at 333.215 kb on + strand, within Psyr_0313 at 333.215 kb on + strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.441 kb on - strand, within Psyr_0313 at 333.504 kb on + strand, within Psyr_0313 at 333.505 kb on - strand, within Psyr_0313 at 333.505 kb on - strand, within Psyr_0313 at 333.505 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Piperic 10 mM remove 331,399 + Psyr_0310 0.84 -1.0 331,399 + Psyr_0310 0.84 +0.7 331,400 - Psyr_0310 0.85 +0.4 331,400 - Psyr_0310 0.85 -1.1 331,400 - Psyr_0310 0.85 -0.9 331,400 - Psyr_0310 0.85 +0.2 331,400 - Psyr_0310 0.85 -0.2 331,400 - Psyr_0310 0.85 -1.2 331,400 - Psyr_0310 0.85 +0.3 331,477 + Psyr_0310 0.88 -0.7 331,494 + Psyr_0310 0.89 +1.1 331,494 + Psyr_0310 0.89 -1.3 331,498 - Psyr_0310 0.89 -0.6 331,503 + Psyr_0310 0.90 -0.3 331,503 + Psyr_0310 0.90 -0.5 331,504 - Psyr_0310 0.90 -0.5 331,531 + -1.0 331,590 + -0.2 331,590 + +0.6 331,590 + -2.4 331,591 - -0.2 331,591 - -0.5 331,608 + +0.5 331,609 - -0.3 331,648 + +2.6 331,673 - -0.1 331,676 + +0.1 331,676 + +0.1 331,676 + +0.3 331,677 - +0.7 331,677 - -0.1 331,677 - -1.1 331,677 - +0.2 331,678 + -0.3 331,678 + +0.1 331,678 + -0.7 331,678 + -0.1 331,678 + -0.7 331,678 + -1.7 331,679 - -0.2 331,679 - -0.1 331,679 - +0.6 331,679 - +0.6 331,681 - -0.5 331,717 - -0.5 331,719 + -0.2 331,725 + +1.1 331,734 + +1.5 331,766 - -1.3 331,769 + +0.3 331,787 + -1.2 331,788 - +2.5 331,788 - +0.4 331,804 + -0.4 331,804 + +0.0 331,804 + -0.1 332,144 - -0.2 332,144 - +1.5 332,230 + +0.0 332,230 + -4.2 332,230 + -0.5 332,230 + -1.0 332,231 - -0.6 332,246 - -0.0 332,337 + Psyr_0312 0.18 -0.7 332,337 + Psyr_0312 0.18 +0.8 332,338 - Psyr_0312 0.19 -0.4 332,338 - Psyr_0312 0.19 -1.0 332,355 - Psyr_0312 0.23 -1.3 332,355 - Psyr_0312 0.23 +0.6 332,356 + Psyr_0312 0.24 -0.3 332,425 + Psyr_0312 0.43 +1.6 332,425 + Psyr_0312 0.43 -1.0 332,448 - Psyr_0312 0.49 -0.2 332,451 + Psyr_0312 0.50 +1.0 332,451 + Psyr_0312 0.50 -0.7 332,451 + Psyr_0312 0.50 -0.5 332,451 + Psyr_0312 0.50 +0.1 332,452 - Psyr_0312 0.50 -2.7 332,475 - Psyr_0312 0.56 -0.3 332,475 - Psyr_0312 0.56 -0.1 332,488 + Psyr_0312 0.60 -0.4 332,496 - Psyr_0312 0.62 +2.3 332,523 - Psyr_0312 0.70 +0.5 332,550 + Psyr_0312 0.77 -1.8 332,564 + Psyr_0312 0.81 -1.2 332,593 - Psyr_0312 0.89 +0.4 332,593 - Psyr_0312 0.89 -2.8 332,593 - Psyr_0312 0.89 -0.9 332,637 + -1.0 332,637 + +0.3 332,645 - -1.8 332,648 - -2.1 332,660 - -0.7 332,709 + -0.3 332,712 + +0.7 332,712 + +0.5 332,713 - -0.3 332,713 - +0.2 332,713 - -0.3 332,713 - -1.4 332,713 - -0.8 332,713 - -0.2 332,716 + -0.4 332,716 + +0.8 332,717 - -0.1 332,726 + -0.5 332,740 - +1.8 332,772 + -1.1 332,772 + -0.1 332,772 + +3.6 332,772 + -1.1 332,772 + -0.8 332,773 - -1.5 332,773 - -0.4 332,844 + +0.0 332,878 + -1.0 332,878 + +0.1 332,878 + +0.4 332,879 - +0.1 332,879 - -0.2 333,215 + Psyr_0313 0.33 +0.3 333,215 + Psyr_0313 0.33 +0.6 333,216 - Psyr_0313 0.33 -1.0 333,238 - Psyr_0313 0.35 +0.6 333,238 - Psyr_0313 0.35 -0.0 333,303 + Psyr_0313 0.42 -1.1 333,303 + Psyr_0313 0.42 +0.1 333,303 + Psyr_0313 0.42 -0.1 333,303 + Psyr_0313 0.42 -0.4 333,303 + Psyr_0313 0.42 +0.8 333,304 - Psyr_0313 0.42 -0.7 333,304 - Psyr_0313 0.42 +0.1 333,304 - Psyr_0313 0.42 -0.0 333,304 - Psyr_0313 0.42 -0.1 333,441 - Psyr_0313 0.56 -0.9 333,504 + Psyr_0313 0.62 +0.6 333,505 - Psyr_0313 0.63 +0.3 333,505 - Psyr_0313 0.63 -0.1 333,505 - Psyr_0313 0.63 +0.5 333,568 - Psyr_0313 0.69 +0.0 333,568 - Psyr_0313 0.69 +0.4
Or see this region's nucleotide sequence