Experiment: KB with Piperic 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0241 and Psyr_0242 are separated by 47 nucleotides Psyr_0242 and Psyr_0243 are separated by 81 nucleotides Psyr_0243 and Psyr_0244 are separated by 116 nucleotides
Psyr_0241: Psyr_0241 - conserved hypothetical protein, at 253,210 to 255,141
_0241
Psyr_0242: Psyr_0242 - Protein of unknown function DUF962, at 255,189 to 255,713
_0242
Psyr_0243: Psyr_0243 - cyclic nucleotide-binding protein, at 255,795 to 256,505
_0243
Psyr_0244: Psyr_0244 - extracellular solute-binding protein, family 1, at 256,622 to 257,623
_0244
Position (kb) 255
256
257 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 255.088 kb on + strand at 255.088 kb on + strand at 255.088 kb on + strand at 255.089 kb on - strand at 255.089 kb on - strand at 255.190 kb on + strand at 255.191 kb on - strand at 255.191 kb on - strand at 255.358 kb on + strand, within Psyr_0242 at 255.358 kb on + strand, within Psyr_0242 at 255.359 kb on - strand, within Psyr_0242 at 255.359 kb on - strand, within Psyr_0242 at 255.359 kb on - strand, within Psyr_0242 at 255.362 kb on - strand, within Psyr_0242 at 255.464 kb on + strand, within Psyr_0242 at 255.464 kb on + strand, within Psyr_0242 at 255.464 kb on + strand, within Psyr_0242 at 255.465 kb on - strand, within Psyr_0242 at 255.465 kb on - strand, within Psyr_0242 at 255.465 kb on - strand, within Psyr_0242 at 255.465 kb on - strand, within Psyr_0242 at 255.632 kb on + strand, within Psyr_0242 at 255.714 kb on + strand at 255.715 kb on - strand at 255.715 kb on - strand at 255.718 kb on - strand at 255.760 kb on + strand at 255.760 kb on + strand at 255.760 kb on + strand at 255.761 kb on - strand at 255.761 kb on - strand at 255.761 kb on - strand at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 256.293 kb on + strand, within Psyr_0243 at 256.387 kb on + strand, within Psyr_0243 at 256.616 kb on - strand at 256.666 kb on - strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.707 kb on - strand at 256.751 kb on + strand, within Psyr_0244 at 256.751 kb on + strand, within Psyr_0244 at 256.751 kb on + strand, within Psyr_0244 at 256.751 kb on + strand, within Psyr_0244 at 256.752 kb on - strand, within Psyr_0244 at 256.752 kb on - strand, within Psyr_0244 at 256.752 kb on - strand, within Psyr_0244 at 256.915 kb on + strand, within Psyr_0244 at 256.916 kb on - strand, within Psyr_0244 at 256.916 kb on - strand, within Psyr_0244 at 257.009 kb on + strand, within Psyr_0244 at 257.010 kb on - strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.049 kb on - strand, within Psyr_0244 at 257.111 kb on + strand, within Psyr_0244 at 257.112 kb on - strand, within Psyr_0244 at 257.280 kb on - strand, within Psyr_0244 at 257.280 kb on - strand, within Psyr_0244 at 257.282 kb on + strand, within Psyr_0244 at 257.282 kb on + strand, within Psyr_0244 at 257.282 kb on + strand, within Psyr_0244 at 257.283 kb on - strand, within Psyr_0244 at 257.283 kb on - strand, within Psyr_0244 at 257.283 kb on - strand, within Psyr_0244 at 257.285 kb on + strand, within Psyr_0244 at 257.285 kb on + strand, within Psyr_0244 at 257.286 kb on - strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Piperic 10 mM remove 255,088 + -0.7 255,088 + -0.7 255,088 + -0.1 255,089 - -2.1 255,089 - -1.7 255,190 + +1.8 255,191 - -0.9 255,191 - +1.1 255,358 + Psyr_0242 0.32 +1.3 255,358 + Psyr_0242 0.32 +1.3 255,359 - Psyr_0242 0.32 +0.3 255,359 - Psyr_0242 0.32 +0.6 255,359 - Psyr_0242 0.32 +0.4 255,362 - Psyr_0242 0.33 -0.8 255,464 + Psyr_0242 0.52 -0.2 255,464 + Psyr_0242 0.52 +0.2 255,464 + Psyr_0242 0.52 -0.6 255,465 - Psyr_0242 0.53 +0.2 255,465 - Psyr_0242 0.53 -1.0 255,465 - Psyr_0242 0.53 +0.9 255,465 - Psyr_0242 0.53 +2.3 255,632 + Psyr_0242 0.84 -0.2 255,714 + -0.8 255,715 - +0.5 255,715 - +0.8 255,718 - +0.6 255,760 + -0.3 255,760 + -0.5 255,760 + +0.5 255,761 - +0.3 255,761 - -0.1 255,761 - +0.0 255,954 + Psyr_0243 0.22 -1.3 255,954 + Psyr_0243 0.22 -0.7 255,954 + Psyr_0243 0.22 -0.7 255,954 + Psyr_0243 0.22 -1.8 255,954 + Psyr_0243 0.22 +0.6 255,954 + Psyr_0243 0.22 -0.4 255,955 - Psyr_0243 0.23 +0.2 255,955 - Psyr_0243 0.23 -2.9 255,955 - Psyr_0243 0.23 -1.4 255,955 - Psyr_0243 0.23 -0.4 256,293 + Psyr_0243 0.70 +2.3 256,387 + Psyr_0243 0.83 -0.9 256,616 - -0.1 256,666 - -0.2 256,706 + -1.0 256,706 + +0.2 256,707 - +1.8 256,751 + Psyr_0244 0.13 +0.7 256,751 + Psyr_0244 0.13 +1.1 256,751 + Psyr_0244 0.13 -0.0 256,751 + Psyr_0244 0.13 -0.2 256,752 - Psyr_0244 0.13 +1.0 256,752 - Psyr_0244 0.13 -0.3 256,752 - Psyr_0244 0.13 -0.7 256,915 + Psyr_0244 0.29 +0.2 256,916 - Psyr_0244 0.29 +1.5 256,916 - Psyr_0244 0.29 -0.5 257,009 + Psyr_0244 0.39 -1.1 257,010 - Psyr_0244 0.39 -0.3 257,048 + Psyr_0244 0.43 -0.8 257,048 + Psyr_0244 0.43 -0.2 257,048 + Psyr_0244 0.43 -0.9 257,049 - Psyr_0244 0.43 +0.7 257,111 + Psyr_0244 0.49 -0.5 257,112 - Psyr_0244 0.49 +2.5 257,280 - Psyr_0244 0.66 -2.8 257,280 - Psyr_0244 0.66 +0.3 257,282 + Psyr_0244 0.66 -1.1 257,282 + Psyr_0244 0.66 -0.2 257,282 + Psyr_0244 0.66 +0.9 257,283 - Psyr_0244 0.66 -0.5 257,283 - Psyr_0244 0.66 -1.2 257,283 - Psyr_0244 0.66 -1.1 257,285 + Psyr_0244 0.66 +0.8 257,285 + Psyr_0244 0.66 -0.7 257,286 - Psyr_0244 0.66 +0.2 257,393 + Psyr_0244 0.77 +0.5 257,393 + Psyr_0244 0.77 +2.9 257,393 + Psyr_0244 0.77 +0.1 257,394 - Psyr_0244 0.77 +0.4 257,394 - Psyr_0244 0.77 -1.4
Or see this region's nucleotide sequence