Strain Fitness in Escherichia coli BW25113 around b2536

Experiment: Dodecanol and Rhamnolipid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcsiE and hcaT overlap by 9 nucleotideshcaT and hcaR are separated by 159 nucleotides b2535: csiE - orf, hypothetical protein (VIMSS), at 2,663,457 to 2,664,737 csiE b2536: hcaT - predicted 3-phenylpropionic transporter (NCBI), at 2,664,729 to 2,665,868 hcaT b2537: hcaR - DNA-binding transcriptional activator of 3-phenylpropionic acid catabolism (NCBI), at 2,666,028 to 2,666,918 hcaR Position (kb) 2664 2665 2666Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2663.733 kb on - strand, within csiEat 2663.733 kb on - strand, within csiEat 2663.745 kb on - strand, within csiEat 2663.849 kb on - strand, within csiEat 2663.927 kb on + strand, within csiEat 2663.945 kb on - strand, within csiEat 2663.945 kb on - strand, within csiEat 2663.956 kb on - strand, within csiEat 2663.956 kb on - strand, within csiEat 2663.956 kb on - strand, within csiEat 2663.981 kb on + strand, within csiEat 2663.983 kb on - strand, within csiEat 2664.044 kb on + strand, within csiEat 2664.044 kb on + strand, within csiEat 2664.058 kb on + strand, within csiEat 2664.091 kb on - strand, within csiEat 2664.091 kb on - strand, within csiEat 2664.151 kb on - strand, within csiEat 2664.151 kb on - strand, within csiEat 2664.172 kb on - strand, within csiEat 2664.242 kb on + strand, within csiEat 2664.242 kb on + strand, within csiEat 2664.242 kb on + strand, within csiEat 2664.305 kb on - strand, within csiEat 2664.315 kb on - strand, within csiEat 2664.315 kb on - strand, within csiEat 2664.317 kb on + strand, within csiEat 2664.324 kb on + strand, within csiEat 2664.326 kb on + strand, within csiEat 2664.329 kb on + strand, within csiEat 2664.329 kb on + strand, within csiEat 2664.329 kb on + strand, within csiEat 2664.349 kb on + strand, within csiEat 2664.502 kb on - strand, within csiEat 2664.535 kb on - strand, within csiEat 2664.569 kb on + strand, within csiEat 2664.629 kb on + strandat 2664.637 kb on - strandat 2664.637 kb on - strandat 2664.673 kb on + strandat 2664.681 kb on - strandat 2664.681 kb on - strandat 2664.687 kb on + strandat 2664.696 kb on + strandat 2664.732 kb on + strandat 2664.790 kb on + strandat 2664.890 kb on + strand, within hcaTat 2664.933 kb on - strand, within hcaTat 2665.029 kb on + strand, within hcaTat 2665.232 kb on + strand, within hcaTat 2665.232 kb on + strand, within hcaTat 2665.242 kb on + strand, within hcaTat 2665.242 kb on + strand, within hcaTat 2665.318 kb on - strand, within hcaTat 2665.358 kb on + strand, within hcaTat 2665.358 kb on + strand, within hcaTat 2665.375 kb on + strand, within hcaTat 2665.375 kb on + strand, within hcaTat 2665.383 kb on - strand, within hcaTat 2665.518 kb on - strand, within hcaTat 2665.518 kb on - strand, within hcaTat 2665.530 kb on - strand, within hcaTat 2665.530 kb on - strand, within hcaTat 2665.556 kb on + strand, within hcaTat 2665.556 kb on + strand, within hcaTat 2665.794 kb on - strandat 2665.794 kb on - strandat 2665.833 kb on + strandat 2665.833 kb on + strandat 2665.896 kb on - strandat 2665.896 kb on - strandat 2665.896 kb on - strandat 2665.896 kb on - strandat 2665.960 kb on + strandat 2666.111 kb on - strandat 2666.203 kb on - strand, within hcaRat 2666.213 kb on + strand, within hcaRat 2666.213 kb on + strand, within hcaRat 2666.216 kb on - strand, within hcaRat 2666.269 kb on - strand, within hcaRat 2666.334 kb on - strand, within hcaRat 2666.359 kb on + strand, within hcaRat 2666.361 kb on + strand, within hcaRat 2666.389 kb on - strand, within hcaRat 2666.389 kb on - strand, within hcaRat 2666.551 kb on + strand, within hcaRat 2666.563 kb on + strand, within hcaRat 2666.824 kb on + strand, within hcaRat 2666.839 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol and Rhamnolipid (C)
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2,663,733 - csiE b2535 0.22 +2.4
2,663,733 - csiE b2535 0.22 +1.4
2,663,745 - csiE b2535 0.22 +0.5
2,663,849 - csiE b2535 0.31 +1.2
2,663,927 + csiE b2535 0.37 +1.3
2,663,945 - csiE b2535 0.38 -0.5
2,663,945 - csiE b2535 0.38 +0.5
2,663,956 - csiE b2535 0.39 -1.3
2,663,956 - csiE b2535 0.39 -0.3
2,663,956 - csiE b2535 0.39 +1.0
2,663,981 + csiE b2535 0.41 -3.2
2,663,983 - csiE b2535 0.41 +0.5
2,664,044 + csiE b2535 0.46 +0.7
2,664,044 + csiE b2535 0.46 -0.6
2,664,058 + csiE b2535 0.47 +0.1
2,664,091 - csiE b2535 0.49 +0.9
2,664,091 - csiE b2535 0.49 +2.2
2,664,151 - csiE b2535 0.54 +2.2
2,664,151 - csiE b2535 0.54 +2.0
2,664,172 - csiE b2535 0.56 +1.0
2,664,242 + csiE b2535 0.61 +1.1
2,664,242 + csiE b2535 0.61 +0.4
2,664,242 + csiE b2535 0.61 +3.6
2,664,305 - csiE b2535 0.66 +1.1
2,664,315 - csiE b2535 0.67 +1.2
2,664,315 - csiE b2535 0.67 +1.4
2,664,317 + csiE b2535 0.67 +1.2
2,664,324 + csiE b2535 0.68 +2.1
2,664,326 + csiE b2535 0.68 +1.4
2,664,329 + csiE b2535 0.68 +0.8
2,664,329 + csiE b2535 0.68 +0.7
2,664,329 + csiE b2535 0.68 -1.0
2,664,349 + csiE b2535 0.70 +1.6
2,664,502 - csiE b2535 0.82 +0.4
2,664,535 - csiE b2535 0.84 -0.8
2,664,569 + csiE b2535 0.87 +0.8
2,664,629 + -1.2
2,664,637 - +2.1
2,664,637 - +2.4
2,664,673 + +2.3
2,664,681 - +1.1
2,664,681 - +0.5
2,664,687 + +4.3
2,664,696 + +0.6
2,664,732 + +1.1
2,664,790 + +0.5
2,664,890 + hcaT b2536 0.14 -0.8
2,664,933 - hcaT b2536 0.18 -1.3
2,665,029 + hcaT b2536 0.26 -0.1
2,665,232 + hcaT b2536 0.44 -2.6
2,665,232 + hcaT b2536 0.44 +0.8
2,665,242 + hcaT b2536 0.45 +1.6
2,665,242 + hcaT b2536 0.45 +1.3
2,665,318 - hcaT b2536 0.52 +0.4
2,665,358 + hcaT b2536 0.55 +0.6
2,665,358 + hcaT b2536 0.55 +0.8
2,665,375 + hcaT b2536 0.57 +0.3
2,665,375 + hcaT b2536 0.57 -0.3
2,665,383 - hcaT b2536 0.57 +0.6
2,665,518 - hcaT b2536 0.69 -1.3
2,665,518 - hcaT b2536 0.69 -0.9
2,665,530 - hcaT b2536 0.70 -1.2
2,665,530 - hcaT b2536 0.70 -3.7
2,665,556 + hcaT b2536 0.73 +0.5
2,665,556 + hcaT b2536 0.73 +0.3
2,665,794 - +0.9
2,665,794 - -0.5
2,665,833 + +0.9
2,665,833 + -0.3
2,665,896 - +2.2
2,665,896 - +1.1
2,665,896 - -2.4
2,665,896 - +0.1
2,665,960 + -0.3
2,666,111 - -0.1
2,666,203 - hcaR b2537 0.20 +1.0
2,666,213 + hcaR b2537 0.21 -0.3
2,666,213 + hcaR b2537 0.21 +0.5
2,666,216 - hcaR b2537 0.21 -0.2
2,666,269 - hcaR b2537 0.27 -0.5
2,666,334 - hcaR b2537 0.34 +0.5
2,666,359 + hcaR b2537 0.37 +0.2
2,666,361 + hcaR b2537 0.37 -2.9
2,666,389 - hcaR b2537 0.41 +0.1
2,666,389 - hcaR b2537 0.41 +0.1
2,666,551 + hcaR b2537 0.59 +0.4
2,666,563 + hcaR b2537 0.60 +0.4
2,666,824 + hcaR b2537 0.89 -2.0
2,666,839 - +1.0

Or see this region's nucleotide sequence