Strain Fitness in Escherichia coli BW25113 around b1131

Experiment: Dodecanol and Rhamnolipid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphoQ and phoP overlap by 1 nucleotidesphoP and purB are separated by 168 nucleotidespurB and hflD are separated by 3 nucleotideshflD and ycfB are separated by 35 nucleotides b1129: phoQ - sensory histidine kinase in two-compoent regulatory system with PhoP (NCBI), at 1,187,539 to 1,188,999 phoQ b1130: phoP - DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI), at 1,188,999 to 1,189,670 phoP b1131: purB - adenylosuccinate lyase (NCBI), at 1,189,839 to 1,191,209 purB b1132: hflD - hypothetical protein (NCBI), at 1,191,213 to 1,191,854 hflD b1133: ycfB - orf, hypothetical protein (VIMSS), at 1,191,890 to 1,192,996 ycfB Position (kb) 1189 1190 1191 1192Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1188.911 kb on + strandat 1188.924 kb on + strandat 1189.023 kb on - strandat 1189.104 kb on + strand, within phoPat 1189.121 kb on + strand, within phoPat 1189.135 kb on - strand, within phoPat 1189.135 kb on - strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.137 kb on + strand, within phoPat 1189.320 kb on - strand, within phoPat 1189.320 kb on - strand, within phoPat 1189.330 kb on - strand, within phoPat 1189.375 kb on - strand, within phoPat 1189.375 kb on - strand, within phoPat 1189.395 kb on + strand, within phoPat 1189.403 kb on - strand, within phoPat 1189.483 kb on + strand, within phoPat 1189.587 kb on + strand, within phoPat 1189.587 kb on + strand, within phoPat 1189.590 kb on + strand, within phoPat 1189.614 kb on - strandat 1189.713 kb on + strandat 1191.335 kb on - strand, within hflDat 1191.604 kb on - strand, within hflDat 1191.744 kb on - strand, within hflDat 1191.776 kb on - strand, within hflDat 1191.776 kb on - strand, within hflDat 1191.780 kb on - strand, within hflD

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol and Rhamnolipid (C)
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1,188,911 + -2.2
1,188,924 + -3.2
1,189,023 - -3.8
1,189,104 + phoP b1130 0.16 -3.6
1,189,121 + phoP b1130 0.18 -3.8
1,189,135 - phoP b1130 0.20 -4.3
1,189,135 - phoP b1130 0.20 -3.0
1,189,137 + phoP b1130 0.21 -4.6
1,189,137 + phoP b1130 0.21 -4.2
1,189,137 + phoP b1130 0.21 -2.4
1,189,320 - phoP b1130 0.48 -4.1
1,189,320 - phoP b1130 0.48 -3.7
1,189,330 - phoP b1130 0.49 -3.5
1,189,375 - phoP b1130 0.56 -1.6
1,189,375 - phoP b1130 0.56 -2.4
1,189,395 + phoP b1130 0.59 -1.3
1,189,403 - phoP b1130 0.60 -2.2
1,189,483 + phoP b1130 0.72 -3.7
1,189,587 + phoP b1130 0.88 -2.5
1,189,587 + phoP b1130 0.88 -3.2
1,189,590 + phoP b1130 0.88 -4.4
1,189,614 - -2.9
1,189,713 + -3.5
1,191,335 - hflD b1132 0.19 -0.7
1,191,604 - hflD b1132 0.61 +0.4
1,191,744 - hflD b1132 0.83 +0.5
1,191,776 - hflD b1132 0.88 -1.5
1,191,776 - hflD b1132 0.88 +0.4
1,191,780 - hflD b1132 0.88 -2.3

Or see this region's nucleotide sequence