Strain Fitness in Escherichia coli BW25113 around b0953

Experiment: Dodecanol and Rhamnolipid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpqiB and ymbA overlap by 4 nucleotidesymbA and rmf are separated by 255 nucleotidesrmf and fabA are separated by 69 nucleotidesfabA and ycbZ are separated by 68 nucleotides b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122 pqiB b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682 ymbA b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105 rmf b0954: fabA - 3-hydroxydecanoyl-ACP dehydratase (NCBI), at 1,015,175 to 1,015,693 fabA b0955: ycbZ - predicted peptidase (NCBI), at 1,015,762 to 1,017,522 ycbZ Position (kb) 1014 1015 1016Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1013.949 kb on - strand, within pqiBat 1013.955 kb on + strand, within pqiBat 1013.955 kb on + strand, within pqiBat 1014.064 kb on - strandat 1014.064 kb on - strandat 1014.159 kb on - strandat 1014.206 kb on + strand, within ymbAat 1014.216 kb on + strand, within ymbAat 1014.216 kb on + strand, within ymbAat 1014.263 kb on + strand, within ymbAat 1014.268 kb on + strand, within ymbAat 1014.303 kb on - strand, within ymbAat 1014.428 kb on - strand, within ymbAat 1014.428 kb on - strand, within ymbAat 1014.587 kb on + strand, within ymbAat 1014.588 kb on + strand, within ymbAat 1014.588 kb on + strand, within ymbAat 1014.619 kb on - strand, within ymbAat 1014.959 kb on + strand, within rmfat 1014.974 kb on + strand, within rmfat 1014.974 kb on + strand, within rmfat 1015.089 kb on + strandat 1015.097 kb on - strandat 1015.736 kb on - strandat 1015.736 kb on - strandat 1015.736 kb on - strandat 1015.740 kb on - strandat 1015.740 kb on - strandat 1015.876 kb on + strandat 1015.878 kb on - strandat 1015.894 kb on - strandat 1015.922 kb on - strandat 1015.935 kb on + strandat 1015.967 kb on - strand, within ycbZat 1015.967 kb on - strand, within ycbZat 1015.989 kb on + strand, within ycbZat 1016.003 kb on + strand, within ycbZat 1016.003 kb on + strand, within ycbZat 1016.012 kb on + strand, within ycbZ

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol and Rhamnolipid (C)
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1,013,949 - pqiB b0951 0.89 +0.3
1,013,955 + pqiB b0951 0.90 +1.1
1,013,955 + pqiB b0951 0.90 +0.6
1,014,064 - +0.2
1,014,064 - +0.1
1,014,159 - -1.0
1,014,206 + ymbA b0952 0.15 +1.1
1,014,216 + ymbA b0952 0.17 +1.9
1,014,216 + ymbA b0952 0.17 +0.4
1,014,263 + ymbA b0952 0.26 -0.6
1,014,268 + ymbA b0952 0.26 +1.4
1,014,303 - ymbA b0952 0.33 +0.1
1,014,428 - ymbA b0952 0.55 -1.2
1,014,428 - ymbA b0952 0.55 +0.7
1,014,587 + ymbA b0952 0.83 +1.5
1,014,588 + ymbA b0952 0.83 +0.6
1,014,588 + ymbA b0952 0.83 +0.2
1,014,619 - ymbA b0952 0.89 +0.1
1,014,959 + rmf b0953 0.12 +0.5
1,014,974 + rmf b0953 0.21 +0.4
1,014,974 + rmf b0953 0.21 +0.1
1,015,089 + +0.6
1,015,097 - +1.0
1,015,736 - -0.6
1,015,736 - +0.6
1,015,736 - +0.7
1,015,740 - +0.6
1,015,740 - +0.7
1,015,876 + -0.1
1,015,878 - -0.7
1,015,894 - -3.0
1,015,922 - -0.3
1,015,935 + -1.5
1,015,967 - ycbZ b0955 0.12 -0.0
1,015,967 - ycbZ b0955 0.12 +1.5
1,015,989 + ycbZ b0955 0.13 +2.3
1,016,003 + ycbZ b0955 0.14 +0.8
1,016,003 + ycbZ b0955 0.14 +0.9
1,016,012 + ycbZ b0955 0.14 +0.4

Or see this region's nucleotide sequence