Experiment: KB with Chrysophanol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3294 and Psyr_3295 are separated by 204 nucleotides Psyr_3295 and Psyr_3296 are separated by 57 nucleotides Psyr_3296 and Psyr_3297 are separated by 102 nucleotides
Psyr_3294: Psyr_3294 - ABC transporter, at 3,940,713 to 3,942,623
_3294
Psyr_3295: Psyr_3295 - SLT, at 3,942,828 to 3,944,756
_3295
Psyr_3296: Psyr_3296 - MOSC:MOSC, N-terminal beta barrel, at 3,944,814 to 3,945,623
_3296
Psyr_3297: Psyr_3297 - CheW protein, at 3,945,726 to 3,946,652
_3297
Position (kb)
3942
3943
3944
3945 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3942.323 kb on - strand, within Psyr_3294 at 3942.477 kb on + strand at 3942.478 kb on - strand at 3942.662 kb on + strand at 3942.728 kb on - strand at 3942.728 kb on - strand at 3942.785 kb on - strand at 3942.789 kb on + strand at 3942.789 kb on + strand at 3942.789 kb on + strand at 3942.789 kb on + strand at 3942.789 kb on + strand at 3942.789 kb on + strand at 3942.790 kb on - strand at 3942.790 kb on - strand at 3942.790 kb on - strand at 3942.795 kb on + strand at 3942.925 kb on - strand at 3942.925 kb on - strand at 3942.928 kb on - strand at 3942.981 kb on + strand at 3942.981 kb on + strand at 3942.981 kb on + strand at 3942.982 kb on - strand at 3942.982 kb on - strand at 3942.982 kb on - strand at 3942.982 kb on - strand at 3942.982 kb on - strand at 3943.179 kb on + strand, within Psyr_3295 at 3943.179 kb on + strand, within Psyr_3295 at 3943.179 kb on + strand, within Psyr_3295 at 3943.180 kb on - strand, within Psyr_3295 at 3943.180 kb on - strand, within Psyr_3295 at 3943.407 kb on + strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.408 kb on - strand, within Psyr_3295 at 3943.525 kb on + strand, within Psyr_3295 at 3943.614 kb on + strand, within Psyr_3295 at 3943.725 kb on + strand, within Psyr_3295 at 3943.726 kb on - strand, within Psyr_3295 at 3943.726 kb on - strand, within Psyr_3295 at 3943.807 kb on - strand, within Psyr_3295 at 3943.921 kb on - strand, within Psyr_3295 at 3943.921 kb on - strand, within Psyr_3295 at 3943.921 kb on - strand, within Psyr_3295 at 3944.038 kb on + strand, within Psyr_3295 at 3944.038 kb on + strand, within Psyr_3295 at 3944.038 kb on + strand, within Psyr_3295 at 3944.039 kb on - strand, within Psyr_3295 at 3944.172 kb on + strand, within Psyr_3295 at 3944.187 kb on + strand, within Psyr_3295 at 3944.187 kb on + strand, within Psyr_3295 at 3944.187 kb on + strand, within Psyr_3295 at 3944.188 kb on - strand, within Psyr_3295 at 3944.427 kb on + strand, within Psyr_3295 at 3944.427 kb on + strand, within Psyr_3295 at 3944.655 kb on + strand at 3944.655 kb on + strand at 3944.656 kb on - strand at 3944.656 kb on - strand at 3944.656 kb on - strand at 3944.660 kb on - strand at 3944.660 kb on - strand at 3944.660 kb on - strand at 3944.660 kb on - strand at 3944.668 kb on + strand at 3944.668 kb on + strand at 3944.668 kb on + strand at 3944.668 kb on + strand at 3944.668 kb on + strand at 3944.668 kb on + strand at 3944.669 kb on - strand at 3944.677 kb on - strand at 3944.688 kb on + strand at 3944.689 kb on - strand at 3945.048 kb on - strand, within Psyr_3296 at 3945.250 kb on + strand, within Psyr_3296 at 3945.250 kb on + strand, within Psyr_3296 at 3945.421 kb on - strand, within Psyr_3296 at 3945.618 kb on + strand at 3945.619 kb on - strand at 3945.619 kb on - strand at 3945.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Chrysophanol 10 mM remove 3,942,323 - Psyr_3294 0.84 +0.5 3,942,477 + -0.4 3,942,478 - +0.2 3,942,662 + -0.1 3,942,728 - -1.1 3,942,728 - +0.9 3,942,785 - -0.4 3,942,789 + +1.2 3,942,789 + +0.9 3,942,789 + -0.8 3,942,789 + -1.3 3,942,789 + -0.8 3,942,789 + +0.4 3,942,790 - +2.2 3,942,790 - -0.2 3,942,790 - -0.5 3,942,795 + -0.3 3,942,925 - -0.4 3,942,925 - -0.7 3,942,928 - +0.5 3,942,981 + -0.4 3,942,981 + -0.3 3,942,981 + +0.7 3,942,982 - -0.2 3,942,982 - -0.2 3,942,982 - +0.3 3,942,982 - -0.1 3,942,982 - +1.2 3,943,179 + Psyr_3295 0.18 -0.7 3,943,179 + Psyr_3295 0.18 +1.2 3,943,179 + Psyr_3295 0.18 +1.6 3,943,180 - Psyr_3295 0.18 +0.6 3,943,180 - Psyr_3295 0.18 +0.6 3,943,407 + Psyr_3295 0.30 -0.6 3,943,408 - Psyr_3295 0.30 -2.2 3,943,408 - Psyr_3295 0.30 +0.2 3,943,408 - Psyr_3295 0.30 +2.2 3,943,408 - Psyr_3295 0.30 -1.1 3,943,408 - Psyr_3295 0.30 -0.2 3,943,408 - Psyr_3295 0.30 -0.9 3,943,408 - Psyr_3295 0.30 -0.5 3,943,408 - Psyr_3295 0.30 -0.7 3,943,525 + Psyr_3295 0.36 -0.2 3,943,614 + Psyr_3295 0.41 -0.7 3,943,725 + Psyr_3295 0.47 +0.3 3,943,726 - Psyr_3295 0.47 -0.5 3,943,726 - Psyr_3295 0.47 -0.2 3,943,807 - Psyr_3295 0.51 -0.8 3,943,921 - Psyr_3295 0.57 -0.2 3,943,921 - Psyr_3295 0.57 -1.2 3,943,921 - Psyr_3295 0.57 +2.5 3,944,038 + Psyr_3295 0.63 +0.3 3,944,038 + Psyr_3295 0.63 -0.7 3,944,038 + Psyr_3295 0.63 -0.3 3,944,039 - Psyr_3295 0.63 -1.2 3,944,172 + Psyr_3295 0.70 -0.7 3,944,187 + Psyr_3295 0.70 -1.0 3,944,187 + Psyr_3295 0.70 -1.1 3,944,187 + Psyr_3295 0.70 -2.4 3,944,188 - Psyr_3295 0.71 +2.0 3,944,427 + Psyr_3295 0.83 -0.2 3,944,427 + Psyr_3295 0.83 -1.2 3,944,655 + +0.2 3,944,655 + +1.3 3,944,656 - -1.1 3,944,656 - +1.6 3,944,656 - -1.4 3,944,660 - +1.3 3,944,660 - -0.3 3,944,660 - +0.3 3,944,660 - -0.4 3,944,668 + -2.5 3,944,668 + +0.2 3,944,668 + -0.4 3,944,668 + +1.2 3,944,668 + +0.1 3,944,668 + +0.8 3,944,669 - +0.7 3,944,677 - -1.4 3,944,688 + -0.7 3,944,689 - +1.6 3,945,048 - Psyr_3296 0.29 -0.2 3,945,250 + Psyr_3296 0.54 -0.9 3,945,250 + Psyr_3296 0.54 -0.1 3,945,421 - Psyr_3296 0.75 -0.0 3,945,618 + +0.3 3,945,619 - +3.0 3,945,619 - -0.4 3,945,619 - -0.3
Or see this region's nucleotide sequence