Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_2396

Experiment: KB with Chrysophanol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2394 and Psyr_2395 are separated by 101 nucleotidesPsyr_2395 and Psyr_2396 overlap by 4 nucleotidesPsyr_2396 and Psyr_2397 are separated by 97 nucleotides Psyr_2394: Psyr_2394 - succinate semialdehyde dehydrogenase, at 2,775,689 to 2,777,080 _2394 Psyr_2395: Psyr_2395 - transcriptional regulator, LysR family, at 2,777,182 to 2,778,057 _2395 Psyr_2396: Psyr_2396 - Tartrate dehydrogenase, at 2,778,054 to 2,779,175 _2396 Psyr_2397: Psyr_2397 - transcriptional regulator, LysR family, at 2,779,273 to 2,780,187 _2397 Position (kb) 2778 2779 2780Strain fitness (log2 ratio) -1 0 1 2 3 4at 2777.074 kb on + strandat 2777.075 kb on - strandat 2777.075 kb on - strandat 2777.116 kb on + strandat 2777.116 kb on + strandat 2777.118 kb on + strandat 2777.118 kb on + strandat 2777.118 kb on + strandat 2777.118 kb on + strandat 2777.119 kb on - strandat 2777.119 kb on - strandat 2777.119 kb on - strandat 2777.380 kb on + strand, within Psyr_2395at 2777.512 kb on + strand, within Psyr_2395at 2777.513 kb on - strand, within Psyr_2395at 2777.592 kb on + strand, within Psyr_2395at 2777.733 kb on + strand, within Psyr_2395at 2777.733 kb on + strand, within Psyr_2395at 2777.733 kb on + strand, within Psyr_2395at 2777.733 kb on + strand, within Psyr_2395at 2777.733 kb on + strand, within Psyr_2395at 2777.734 kb on - strand, within Psyr_2395at 2777.734 kb on - strand, within Psyr_2395at 2777.734 kb on - strand, within Psyr_2395at 2777.734 kb on - strand, within Psyr_2395at 2777.734 kb on - strand, within Psyr_2395at 2777.734 kb on - strand, within Psyr_2395at 2777.738 kb on - strand, within Psyr_2395at 2777.738 kb on - strand, within Psyr_2395at 2777.738 kb on - strand, within Psyr_2395at 2777.834 kb on - strand, within Psyr_2395at 2777.834 kb on - strand, within Psyr_2395at 2778.997 kb on + strand, within Psyr_2396at 2778.997 kb on + strand, within Psyr_2396at 2779.021 kb on + strand, within Psyr_2396at 2779.022 kb on - strand, within Psyr_2396at 2779.128 kb on - strandat 2779.216 kb on + strandat 2779.231 kb on + strandat 2779.238 kb on - strandat 2779.278 kb on + strandat 2779.375 kb on + strand, within Psyr_2397at 2779.375 kb on + strand, within Psyr_2397at 2779.389 kb on + strand, within Psyr_2397at 2779.390 kb on - strand, within Psyr_2397at 2779.390 kb on - strand, within Psyr_2397at 2779.390 kb on - strand, within Psyr_2397at 2779.477 kb on + strand, within Psyr_2397at 2779.478 kb on - strand, within Psyr_2397at 2780.152 kb on + strandat 2780.152 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Chrysophanol 10 mM
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2,777,074 + +0.7
2,777,075 - -0.3
2,777,075 - -0.0
2,777,116 + -0.9
2,777,116 + +0.7
2,777,118 + +2.0
2,777,118 + -0.2
2,777,118 + +0.0
2,777,118 + +0.2
2,777,119 - +2.8
2,777,119 - +1.2
2,777,119 - -0.6
2,777,380 + Psyr_2395 0.23 +2.0
2,777,512 + Psyr_2395 0.38 +2.8
2,777,513 - Psyr_2395 0.38 +1.6
2,777,592 + Psyr_2395 0.47 -0.4
2,777,733 + Psyr_2395 0.63 +0.9
2,777,733 + Psyr_2395 0.63 +0.0
2,777,733 + Psyr_2395 0.63 +0.4
2,777,733 + Psyr_2395 0.63 +0.5
2,777,733 + Psyr_2395 0.63 +0.9
2,777,734 - Psyr_2395 0.63 -0.2
2,777,734 - Psyr_2395 0.63 -0.3
2,777,734 - Psyr_2395 0.63 +1.0
2,777,734 - Psyr_2395 0.63 -0.7
2,777,734 - Psyr_2395 0.63 +3.2
2,777,734 - Psyr_2395 0.63 +0.0
2,777,738 - Psyr_2395 0.63 +1.2
2,777,738 - Psyr_2395 0.63 +1.1
2,777,738 - Psyr_2395 0.63 -1.3
2,777,834 - Psyr_2395 0.74 -0.4
2,777,834 - Psyr_2395 0.74 -0.6
2,778,997 + Psyr_2396 0.84 -0.2
2,778,997 + Psyr_2396 0.84 +3.6
2,779,021 + Psyr_2396 0.86 -1.6
2,779,022 - Psyr_2396 0.86 -1.5
2,779,128 - -1.1
2,779,216 + +0.7
2,779,231 + +0.9
2,779,238 - +0.3
2,779,278 + +0.7
2,779,375 + Psyr_2397 0.11 +1.5
2,779,375 + Psyr_2397 0.11 +3.8
2,779,389 + Psyr_2397 0.13 -1.7
2,779,390 - Psyr_2397 0.13 -0.4
2,779,390 - Psyr_2397 0.13 -1.4
2,779,390 - Psyr_2397 0.13 +0.4
2,779,477 + Psyr_2397 0.22 +3.0
2,779,478 - Psyr_2397 0.22 +0.8
2,780,152 + +1.6
2,780,152 + -1.6

Or see this region's nucleotide sequence