Experiment: KB with Chrysophanol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1873 and Psyr_1874 are separated by 63 nucleotides Psyr_1874 and Psyr_1875 are separated by 123 nucleotides Psyr_1875 and Psyr_1876 are separated by 125 nucleotides
Psyr_1873: Psyr_1873 - Glutamine amidotransferase class-I, at 2,148,483 to 2,149,211
_1873
Psyr_1874: Psyr_1874 - Mg2+ transporter protein, CorA-like protein, at 2,149,275 to 2,150,246
_1874
Psyr_1875: Psyr_1875 - Enoyl-CoA hydratase/isomerase, at 2,150,370 to 2,151,059
_1875
Psyr_1876: Psyr_1876 - conserved hypothetical protein, at 2,151,185 to 2,151,478
_1876
Position (kb)
2149
2150
2151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2148.372 kb on + strand at 2148.381 kb on + strand at 2148.452 kb on - strand at 2148.462 kb on + strand at 2148.463 kb on - strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.500 kb on + strand at 2148.543 kb on + strand at 2148.572 kb on - strand, within Psyr_1873 at 2148.612 kb on + strand, within Psyr_1873 at 2148.613 kb on - strand, within Psyr_1873 at 2148.634 kb on - strand, within Psyr_1873 at 2148.663 kb on + strand, within Psyr_1873 at 2148.730 kb on - strand, within Psyr_1873 at 2148.730 kb on - strand, within Psyr_1873 at 2148.730 kb on - strand, within Psyr_1873 at 2148.744 kb on + strand, within Psyr_1873 at 2149.005 kb on - strand, within Psyr_1873 at 2149.107 kb on + strand, within Psyr_1873 at 2149.107 kb on + strand, within Psyr_1873 at 2149.279 kb on - strand at 2149.316 kb on + strand at 2149.351 kb on + strand at 2149.351 kb on + strand at 2149.399 kb on + strand, within Psyr_1874 at 2149.399 kb on + strand, within Psyr_1874 at 2149.400 kb on - strand, within Psyr_1874 at 2149.400 kb on - strand, within Psyr_1874 at 2149.675 kb on - strand, within Psyr_1874 at 2149.792 kb on + strand, within Psyr_1874 at 2149.792 kb on + strand, within Psyr_1874 at 2149.792 kb on + strand, within Psyr_1874 at 2149.793 kb on - strand, within Psyr_1874 at 2149.793 kb on - strand, within Psyr_1874 at 2149.822 kb on + strand, within Psyr_1874 at 2149.925 kb on + strand, within Psyr_1874 at 2149.926 kb on - strand, within Psyr_1874 at 2149.927 kb on + strand, within Psyr_1874 at 2149.927 kb on + strand, within Psyr_1874 at 2149.927 kb on + strand, within Psyr_1874 at 2149.927 kb on + strand, within Psyr_1874 at 2149.928 kb on - strand, within Psyr_1874 at 2149.928 kb on - strand, within Psyr_1874 at 2149.947 kb on - strand, within Psyr_1874 at 2149.947 kb on - strand, within Psyr_1874 at 2149.947 kb on - strand, within Psyr_1874 at 2149.947 kb on - strand, within Psyr_1874 at 2149.993 kb on + strand, within Psyr_1874 at 2149.994 kb on - strand, within Psyr_1874 at 2149.994 kb on - strand, within Psyr_1874 at 2149.994 kb on - strand, within Psyr_1874 at 2149.994 kb on - strand, within Psyr_1874 at 2149.994 kb on - strand, within Psyr_1874 at 2149.994 kb on - strand, within Psyr_1874 at 2150.218 kb on + strand at 2150.219 kb on - strand at 2150.319 kb on + strand at 2150.320 kb on - strand at 2150.320 kb on - strand at 2150.465 kb on + strand, within Psyr_1875 at 2150.466 kb on - strand, within Psyr_1875 at 2150.915 kb on + strand, within Psyr_1875 at 2150.916 kb on - strand, within Psyr_1875 at 2150.916 kb on - strand, within Psyr_1875 at 2151.040 kb on + strand at 2151.041 kb on - strand at 2151.073 kb on + strand at 2151.074 kb on - strand at 2151.092 kb on + strand at 2151.114 kb on - strand at 2151.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Chrysophanol 10 mM remove 2,148,372 + +0.4 2,148,381 + -0.7 2,148,452 - +0.5 2,148,462 + +0.5 2,148,463 - -0.6 2,148,482 + +0.4 2,148,482 + -2.8 2,148,482 + -0.1 2,148,482 + +0.4 2,148,482 + -1.4 2,148,482 + +1.0 2,148,482 + +1.6 2,148,482 + +0.9 2,148,482 + +1.2 2,148,483 - +0.9 2,148,483 - -1.0 2,148,483 - -0.3 2,148,483 - +1.2 2,148,483 - +2.6 2,148,483 - -0.3 2,148,483 - -1.8 2,148,500 + -0.3 2,148,543 + +1.2 2,148,572 - Psyr_1873 0.12 +0.9 2,148,612 + Psyr_1873 0.18 -0.1 2,148,613 - Psyr_1873 0.18 -1.6 2,148,634 - Psyr_1873 0.21 -0.8 2,148,663 + Psyr_1873 0.25 -0.1 2,148,730 - Psyr_1873 0.34 +4.3 2,148,730 - Psyr_1873 0.34 +0.9 2,148,730 - Psyr_1873 0.34 +0.3 2,148,744 + Psyr_1873 0.36 -0.6 2,149,005 - Psyr_1873 0.72 -1.1 2,149,107 + Psyr_1873 0.86 -0.2 2,149,107 + Psyr_1873 0.86 +2.0 2,149,279 - -0.4 2,149,316 + -1.4 2,149,351 + -1.3 2,149,351 + -1.3 2,149,399 + Psyr_1874 0.13 +0.2 2,149,399 + Psyr_1874 0.13 +0.2 2,149,400 - Psyr_1874 0.13 +0.8 2,149,400 - Psyr_1874 0.13 -0.5 2,149,675 - Psyr_1874 0.41 -1.2 2,149,792 + Psyr_1874 0.53 +0.4 2,149,792 + Psyr_1874 0.53 -0.6 2,149,792 + Psyr_1874 0.53 +1.6 2,149,793 - Psyr_1874 0.53 -2.5 2,149,793 - Psyr_1874 0.53 +0.7 2,149,822 + Psyr_1874 0.56 -0.2 2,149,925 + Psyr_1874 0.67 +1.1 2,149,926 - Psyr_1874 0.67 +1.2 2,149,927 + Psyr_1874 0.67 +1.6 2,149,927 + Psyr_1874 0.67 -0.2 2,149,927 + Psyr_1874 0.67 +1.3 2,149,927 + Psyr_1874 0.67 -1.1 2,149,928 - Psyr_1874 0.67 -1.2 2,149,928 - Psyr_1874 0.67 -0.6 2,149,947 - Psyr_1874 0.69 +2.7 2,149,947 - Psyr_1874 0.69 -0.2 2,149,947 - Psyr_1874 0.69 +0.8 2,149,947 - Psyr_1874 0.69 +1.2 2,149,993 + Psyr_1874 0.74 -1.3 2,149,994 - Psyr_1874 0.74 -0.4 2,149,994 - Psyr_1874 0.74 -0.4 2,149,994 - Psyr_1874 0.74 +1.7 2,149,994 - Psyr_1874 0.74 +1.7 2,149,994 - Psyr_1874 0.74 +0.5 2,149,994 - Psyr_1874 0.74 -0.8 2,150,218 + +0.6 2,150,219 - -0.8 2,150,319 + -2.4 2,150,320 - +0.2 2,150,320 - -1.1 2,150,465 + Psyr_1875 0.14 +0.2 2,150,466 - Psyr_1875 0.14 -0.0 2,150,915 + Psyr_1875 0.79 +1.2 2,150,916 - Psyr_1875 0.79 +0.7 2,150,916 - Psyr_1875 0.79 +4.6 2,151,040 + -0.3 2,151,041 - -0.4 2,151,073 + -0.1 2,151,074 - -0.8 2,151,092 + -0.2 2,151,114 - +0.1 2,151,114 - +0.5
Or see this region's nucleotide sequence