Experiment: KB with Chrysophanol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1188 and Psyr_1189 are separated by 219 nucleotides
Psyr_1188: Psyr_1188 - type III effector protein AvrE1, at 1,343,119 to 1,348,479
_1188
Psyr_1189: Psyr_1189 - SLT:MLTD_N, at 1,348,699 to 1,350,222
_1189
Position (kb)
1348
1349
1350
1351 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1347.729 kb on + strand, within Psyr_1188 at 1347.796 kb on - strand, within Psyr_1188 at 1347.811 kb on - strand, within Psyr_1188 at 1347.880 kb on + strand, within Psyr_1188 at 1347.881 kb on - strand, within Psyr_1188 at 1348.096 kb on + strand at 1348.097 kb on - strand at 1348.097 kb on - strand at 1348.097 kb on - strand at 1348.097 kb on - strand at 1348.180 kb on - strand at 1348.260 kb on + strand at 1348.633 kb on - strand at 1348.921 kb on + strand, within Psyr_1189 at 1348.921 kb on + strand, within Psyr_1189 at 1348.921 kb on + strand, within Psyr_1189 at 1348.922 kb on - strand, within Psyr_1189 at 1348.995 kb on + strand, within Psyr_1189 at 1349.068 kb on + strand, within Psyr_1189 at 1349.149 kb on + strand, within Psyr_1189 at 1349.149 kb on + strand, within Psyr_1189 at 1349.149 kb on + strand, within Psyr_1189 at 1349.150 kb on - strand, within Psyr_1189 at 1349.150 kb on - strand, within Psyr_1189 at 1349.150 kb on - strand, within Psyr_1189 at 1349.242 kb on - strand, within Psyr_1189 at 1349.245 kb on + strand, within Psyr_1189 at 1349.246 kb on - strand, within Psyr_1189 at 1349.293 kb on + strand, within Psyr_1189 at 1349.351 kb on - strand, within Psyr_1189 at 1349.455 kb on + strand, within Psyr_1189 at 1349.455 kb on + strand, within Psyr_1189 at 1349.455 kb on + strand, within Psyr_1189 at 1349.455 kb on + strand, within Psyr_1189 at 1349.455 kb on + strand, within Psyr_1189 at 1349.456 kb on - strand, within Psyr_1189 at 1349.456 kb on - strand, within Psyr_1189 at 1349.543 kb on - strand, within Psyr_1189 at 1349.607 kb on - strand, within Psyr_1189 at 1349.718 kb on + strand, within Psyr_1189 at 1350.012 kb on - strand, within Psyr_1189 at 1350.012 kb on - strand, within Psyr_1189 at 1350.012 kb on - strand, within Psyr_1189 at 1350.012 kb on - strand, within Psyr_1189 at 1350.106 kb on + strand at 1350.106 kb on + strand at 1350.106 kb on + strand at 1350.106 kb on + strand at 1350.106 kb on + strand at 1350.107 kb on - strand at 1350.107 kb on - strand at 1350.107 kb on - strand at 1350.107 kb on - strand at 1350.348 kb on - strand at 1350.358 kb on - strand at 1350.358 kb on - strand at 1350.388 kb on + strand at 1350.389 kb on - strand at 1350.389 kb on - strand at 1350.389 kb on - strand at 1350.401 kb on - strand at 1350.429 kb on - strand at 1350.436 kb on + strand at 1350.440 kb on + strand at 1350.440 kb on + strand at 1350.441 kb on - strand at 1350.513 kb on + strand at 1350.513 kb on + strand at 1350.514 kb on - strand at 1350.514 kb on - strand at 1350.514 kb on - strand at 1350.514 kb on - strand at 1350.515 kb on + strand at 1350.516 kb on - strand at 1350.516 kb on - strand at 1350.516 kb on - strand at 1350.540 kb on - strand at 1350.563 kb on - strand at 1350.563 kb on - strand at 1350.563 kb on - strand at 1350.585 kb on + strand at 1350.735 kb on + strand at 1350.736 kb on - strand at 1350.937 kb on - strand at 1350.992 kb on + strand at 1351.094 kb on - strand at 1351.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Chrysophanol 10 mM remove 1,347,729 + Psyr_1188 0.86 -0.6 1,347,796 - Psyr_1188 0.87 -0.8 1,347,811 - Psyr_1188 0.88 -0.2 1,347,880 + Psyr_1188 0.89 +0.5 1,347,881 - Psyr_1188 0.89 +0.3 1,348,096 + -0.2 1,348,097 - -0.9 1,348,097 - +1.0 1,348,097 - -0.3 1,348,097 - -0.1 1,348,180 - -0.3 1,348,260 + -1.3 1,348,633 - +0.8 1,348,921 + Psyr_1189 0.15 -0.1 1,348,921 + Psyr_1189 0.15 -0.8 1,348,921 + Psyr_1189 0.15 +0.3 1,348,922 - Psyr_1189 0.15 -1.8 1,348,995 + Psyr_1189 0.19 -0.9 1,349,068 + Psyr_1189 0.24 +0.6 1,349,149 + Psyr_1189 0.30 -0.3 1,349,149 + Psyr_1189 0.30 -1.4 1,349,149 + Psyr_1189 0.30 +0.7 1,349,150 - Psyr_1189 0.30 -0.9 1,349,150 - Psyr_1189 0.30 +0.4 1,349,150 - Psyr_1189 0.30 -2.7 1,349,242 - Psyr_1189 0.36 -0.8 1,349,245 + Psyr_1189 0.36 +3.2 1,349,246 - Psyr_1189 0.36 +0.3 1,349,293 + Psyr_1189 0.39 -0.7 1,349,351 - Psyr_1189 0.43 +0.4 1,349,455 + Psyr_1189 0.50 +0.2 1,349,455 + Psyr_1189 0.50 -0.2 1,349,455 + Psyr_1189 0.50 -0.8 1,349,455 + Psyr_1189 0.50 +0.4 1,349,455 + Psyr_1189 0.50 +0.0 1,349,456 - Psyr_1189 0.50 +2.7 1,349,456 - Psyr_1189 0.50 +0.0 1,349,543 - Psyr_1189 0.55 -0.1 1,349,607 - Psyr_1189 0.60 +0.6 1,349,718 + Psyr_1189 0.67 +0.3 1,350,012 - Psyr_1189 0.86 +1.7 1,350,012 - Psyr_1189 0.86 -0.0 1,350,012 - Psyr_1189 0.86 -0.1 1,350,012 - Psyr_1189 0.86 +0.4 1,350,106 + -0.3 1,350,106 + -2.6 1,350,106 + -0.4 1,350,106 + +0.2 1,350,106 + +0.9 1,350,107 - -0.9 1,350,107 - -1.1 1,350,107 - -0.7 1,350,107 - -1.6 1,350,348 - -1.2 1,350,358 - -0.4 1,350,358 - +0.3 1,350,388 + +2.1 1,350,389 - +1.2 1,350,389 - +2.7 1,350,389 - +2.7 1,350,401 - +0.6 1,350,429 - +0.3 1,350,436 + -0.1 1,350,440 + -2.1 1,350,440 + -1.4 1,350,441 - -0.3 1,350,513 + +0.3 1,350,513 + +0.1 1,350,514 - +2.5 1,350,514 - +0.2 1,350,514 - -1.2 1,350,514 - +1.6 1,350,515 + -1.3 1,350,516 - +0.9 1,350,516 - +0.6 1,350,516 - -1.1 1,350,540 - +2.7 1,350,563 - +1.2 1,350,563 - +2.4 1,350,563 - -0.2 1,350,585 + +2.2 1,350,735 + -0.0 1,350,736 - -0.7 1,350,937 - -0.3 1,350,992 + -1.3 1,351,094 - +0.2 1,351,109 - -0.8
Or see this region's nucleotide sequence