Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_0616

Experiment: KB with Chrysophanol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0614 and Psyr_0615 are separated by 5 nucleotidesPsyr_0615 and Psyr_0616 overlap by 4 nucleotidesPsyr_0616 and Psyr_0617 are separated by 2 nucleotides Psyr_0614: Psyr_0614 - HAD-superfamily hydrolase, subfamily IA, variant 3, at 703,463 to 704,101 _0614 Psyr_0615: Psyr_0615 - conserved hypothetical protein, at 704,107 to 704,829 _0615 Psyr_0616: Psyr_0616 - conserved domain protein, at 704,826 to 705,146 _0616 Psyr_0617: Psyr_0617 - conserved hypothetical protein, at 705,149 to 706,924 _0617 Position (kb) 704 705 706Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 703.847 kb on + strand, within Psyr_0614at 703.848 kb on - strand, within Psyr_0614at 703.848 kb on - strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.907 kb on + strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.908 kb on - strand, within Psyr_0614at 703.909 kb on + strand, within Psyr_0614at 703.910 kb on - strand, within Psyr_0614at 703.910 kb on - strand, within Psyr_0614at 704.037 kb on + strand, within Psyr_0614at 704.037 kb on + strand, within Psyr_0614at 704.112 kb on + strandat 704.113 kb on - strandat 704.173 kb on + strandat 704.173 kb on + strandat 704.173 kb on + strandat 704.173 kb on + strandat 704.173 kb on + strandat 704.174 kb on - strandat 704.185 kb on + strand, within Psyr_0615at 704.185 kb on + strand, within Psyr_0615at 704.185 kb on + strand, within Psyr_0615at 704.186 kb on - strand, within Psyr_0615at 704.186 kb on - strand, within Psyr_0615at 704.208 kb on + strand, within Psyr_0615at 704.208 kb on + strand, within Psyr_0615at 704.208 kb on + strand, within Psyr_0615at 704.209 kb on - strand, within Psyr_0615at 704.209 kb on - strand, within Psyr_0615at 704.209 kb on - strand, within Psyr_0615at 704.209 kb on - strand, within Psyr_0615at 704.209 kb on - strand, within Psyr_0615at 704.209 kb on - strand, within Psyr_0615at 704.216 kb on + strand, within Psyr_0615at 704.216 kb on + strand, within Psyr_0615at 704.216 kb on + strand, within Psyr_0615at 704.217 kb on - strand, within Psyr_0615at 704.232 kb on - strand, within Psyr_0615at 704.334 kb on - strand, within Psyr_0615at 704.334 kb on - strand, within Psyr_0615at 704.459 kb on + strand, within Psyr_0615at 704.459 kb on + strand, within Psyr_0615at 704.460 kb on - strand, within Psyr_0615at 704.460 kb on - strand, within Psyr_0615at 704.460 kb on - strand, within Psyr_0615at 704.460 kb on - strand, within Psyr_0615at 704.460 kb on - strand, within Psyr_0615at 704.527 kb on + strand, within Psyr_0615at 704.695 kb on + strand, within Psyr_0615at 704.695 kb on + strand, within Psyr_0615at 704.695 kb on + strand, within Psyr_0615at 704.695 kb on + strand, within Psyr_0615at 704.696 kb on - strand, within Psyr_0615at 704.696 kb on - strand, within Psyr_0615at 704.696 kb on - strand, within Psyr_0615at 704.696 kb on - strand, within Psyr_0615at 704.696 kb on - strand, within Psyr_0615at 704.699 kb on + strand, within Psyr_0615at 704.699 kb on + strand, within Psyr_0615at 704.699 kb on + strand, within Psyr_0615at 704.700 kb on - strand, within Psyr_0615at 704.700 kb on - strand, within Psyr_0615at 704.700 kb on - strand, within Psyr_0615at 704.928 kb on + strand, within Psyr_0616at 704.928 kb on + strand, within Psyr_0616at 704.928 kb on + strand, within Psyr_0616at 704.929 kb on - strand, within Psyr_0616at 705.135 kb on + strandat 705.135 kb on + strandat 705.135 kb on + strandat 705.135 kb on + strandat 705.135 kb on + strandat 705.135 kb on + strandat 705.135 kb on + strandat 705.136 kb on - strandat 705.136 kb on - strandat 705.136 kb on - strandat 705.136 kb on - strandat 705.136 kb on - strandat 705.136 kb on - strandat 705.188 kb on + strandat 705.189 kb on - strandat 705.189 kb on - strandat 705.192 kb on + strandat 705.228 kb on + strandat 705.338 kb on + strand, within Psyr_0617at 705.339 kb on - strand, within Psyr_0617at 705.349 kb on + strand, within Psyr_0617at 705.354 kb on - strand, within Psyr_0617at 705.354 kb on - strand, within Psyr_0617at 705.441 kb on + strand, within Psyr_0617at 705.474 kb on - strand, within Psyr_0617at 705.488 kb on + strand, within Psyr_0617at 705.489 kb on - strand, within Psyr_0617at 705.539 kb on + strand, within Psyr_0617at 705.540 kb on - strand, within Psyr_0617at 705.540 kb on - strand, within Psyr_0617at 705.540 kb on - strand, within Psyr_0617at 705.550 kb on - strand, within Psyr_0617at 705.684 kb on + strand, within Psyr_0617at 705.684 kb on + strand, within Psyr_0617at 705.684 kb on + strand, within Psyr_0617at 705.684 kb on + strand, within Psyr_0617at 705.685 kb on - strand, within Psyr_0617at 705.685 kb on - strand, within Psyr_0617at 705.685 kb on - strand, within Psyr_0617at 705.685 kb on - strand, within Psyr_0617at 705.710 kb on - strand, within Psyr_0617at 705.710 kb on - strand, within Psyr_0617at 705.720 kb on - strand, within Psyr_0617at 705.720 kb on - strand, within Psyr_0617at 706.050 kb on + strand, within Psyr_0617at 706.100 kb on + strand, within Psyr_0617at 706.100 kb on + strand, within Psyr_0617at 706.101 kb on - strand, within Psyr_0617at 706.136 kb on + strand, within Psyr_0617at 706.137 kb on - strand, within Psyr_0617

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Chrysophanol 10 mM
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703,847 + Psyr_0614 0.60 +3.5
703,848 - Psyr_0614 0.60 +0.0
703,848 - Psyr_0614 0.60 -0.0
703,907 + Psyr_0614 0.69 +0.5
703,907 + Psyr_0614 0.69 -2.0
703,907 + Psyr_0614 0.69 -0.0
703,907 + Psyr_0614 0.69 +1.2
703,907 + Psyr_0614 0.69 -0.4
703,907 + Psyr_0614 0.69 -0.5
703,907 + Psyr_0614 0.69 +0.8
703,907 + Psyr_0614 0.69 -1.1
703,907 + Psyr_0614 0.69 -0.8
703,907 + Psyr_0614 0.69 +0.3
703,907 + Psyr_0614 0.69 +0.6
703,907 + Psyr_0614 0.69 -0.2
703,907 + Psyr_0614 0.69 -0.5
703,907 + Psyr_0614 0.69 -0.2
703,907 + Psyr_0614 0.69 -0.1
703,908 - Psyr_0614 0.70 -0.9
703,908 - Psyr_0614 0.70 +0.6
703,908 - Psyr_0614 0.70 -0.9
703,908 - Psyr_0614 0.70 +0.1
703,908 - Psyr_0614 0.70 -0.7
703,908 - Psyr_0614 0.70 +1.0
703,908 - Psyr_0614 0.70 -1.0
703,908 - Psyr_0614 0.70 -0.8
703,908 - Psyr_0614 0.70 -3.2
703,908 - Psyr_0614 0.70 -0.2
703,908 - Psyr_0614 0.70 +1.8
703,908 - Psyr_0614 0.70 +0.6
703,908 - Psyr_0614 0.70 -0.4
703,908 - Psyr_0614 0.70 -0.2
703,908 - Psyr_0614 0.70 -0.7
703,908 - Psyr_0614 0.70 -0.2
703,908 - Psyr_0614 0.70 +0.4
703,908 - Psyr_0614 0.70 +0.9
703,908 - Psyr_0614 0.70 -0.3
703,908 - Psyr_0614 0.70 +0.2
703,909 + Psyr_0614 0.70 -0.9
703,910 - Psyr_0614 0.70 -0.7
703,910 - Psyr_0614 0.70 +0.7
704,037 + Psyr_0614 0.90 +2.0
704,037 + Psyr_0614 0.90 -0.6
704,112 + -0.5
704,113 - +0.9
704,173 + -0.4
704,173 + -0.3
704,173 + +1.3
704,173 + -0.8
704,173 + -0.2
704,174 - -0.0
704,185 + Psyr_0615 0.11 -2.5
704,185 + Psyr_0615 0.11 -0.6
704,185 + Psyr_0615 0.11 +0.5
704,186 - Psyr_0615 0.11 -0.4
704,186 - Psyr_0615 0.11 +1.2
704,208 + Psyr_0615 0.14 +0.6
704,208 + Psyr_0615 0.14 -0.6
704,208 + Psyr_0615 0.14 -0.8
704,209 - Psyr_0615 0.14 +1.4
704,209 - Psyr_0615 0.14 -0.3
704,209 - Psyr_0615 0.14 -1.0
704,209 - Psyr_0615 0.14 -0.2
704,209 - Psyr_0615 0.14 +1.2
704,209 - Psyr_0615 0.14 +1.2
704,216 + Psyr_0615 0.15 +0.2
704,216 + Psyr_0615 0.15 +0.3
704,216 + Psyr_0615 0.15 +0.5
704,217 - Psyr_0615 0.15 -0.8
704,232 - Psyr_0615 0.17 +1.9
704,334 - Psyr_0615 0.31 +0.9
704,334 - Psyr_0615 0.31 +0.5
704,459 + Psyr_0615 0.49 +4.0
704,459 + Psyr_0615 0.49 +1.0
704,460 - Psyr_0615 0.49 +1.9
704,460 - Psyr_0615 0.49 -1.4
704,460 - Psyr_0615 0.49 +0.0
704,460 - Psyr_0615 0.49 -0.8
704,460 - Psyr_0615 0.49 +0.3
704,527 + Psyr_0615 0.58 -1.0
704,695 + Psyr_0615 0.81 +0.1
704,695 + Psyr_0615 0.81 +0.3
704,695 + Psyr_0615 0.81 -0.0
704,695 + Psyr_0615 0.81 +2.1
704,696 - Psyr_0615 0.81 -0.1
704,696 - Psyr_0615 0.81 -1.6
704,696 - Psyr_0615 0.81 +0.6
704,696 - Psyr_0615 0.81 -2.0
704,696 - Psyr_0615 0.81 +0.5
704,699 + Psyr_0615 0.82 -1.4
704,699 + Psyr_0615 0.82 +0.2
704,699 + Psyr_0615 0.82 -1.1
704,700 - Psyr_0615 0.82 -0.5
704,700 - Psyr_0615 0.82 +0.0
704,700 - Psyr_0615 0.82 -0.1
704,928 + Psyr_0616 0.32 -1.6
704,928 + Psyr_0616 0.32 -0.9
704,928 + Psyr_0616 0.32 -0.2
704,929 - Psyr_0616 0.32 +0.2
705,135 + -0.8
705,135 + -1.4
705,135 + +0.4
705,135 + +1.9
705,135 + +0.1
705,135 + +1.7
705,135 + +0.4
705,136 - +1.1
705,136 - +1.2
705,136 - -0.5
705,136 - +0.1
705,136 - +0.6
705,136 - -0.4
705,188 + -1.8
705,189 - +0.6
705,189 - +0.2
705,192 + -0.7
705,228 + -0.4
705,338 + Psyr_0617 0.11 -0.7
705,339 - Psyr_0617 0.11 -0.7
705,349 + Psyr_0617 0.11 -1.2
705,354 - Psyr_0617 0.12 -1.1
705,354 - Psyr_0617 0.12 +1.4
705,441 + Psyr_0617 0.16 -2.9
705,474 - Psyr_0617 0.18 -0.1
705,488 + Psyr_0617 0.19 +2.2
705,489 - Psyr_0617 0.19 +0.3
705,539 + Psyr_0617 0.22 +0.7
705,540 - Psyr_0617 0.22 -1.1
705,540 - Psyr_0617 0.22 -0.1
705,540 - Psyr_0617 0.22 -0.4
705,550 - Psyr_0617 0.23 -0.6
705,684 + Psyr_0617 0.30 +0.5
705,684 + Psyr_0617 0.30 -1.0
705,684 + Psyr_0617 0.30 -0.1
705,684 + Psyr_0617 0.30 +3.2
705,685 - Psyr_0617 0.30 -2.0
705,685 - Psyr_0617 0.30 +0.3
705,685 - Psyr_0617 0.30 +0.5
705,685 - Psyr_0617 0.30 +0.8
705,710 - Psyr_0617 0.32 -0.0
705,710 - Psyr_0617 0.32 -2.0
705,720 - Psyr_0617 0.32 +0.5
705,720 - Psyr_0617 0.32 +1.2
706,050 + Psyr_0617 0.51 -0.3
706,100 + Psyr_0617 0.54 -1.7
706,100 + Psyr_0617 0.54 +0.1
706,101 - Psyr_0617 0.54 +0.7
706,136 + Psyr_0617 0.56 -0.7
706,137 - Psyr_0617 0.56 +0.1

Or see this region's nucleotide sequence