Strain Fitness in Escherichia coli BW25113 around b2713

Experiment: Dodecanol and Rhamnolipid (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthypF and hydN are separated by 152 nucleotideshydN and ascG are separated by 148 nucleotides b2712: hypF - carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases (NCBI), at 2,833,195 to 2,835,447 hypF b2713: hydN - formate dehydrogenase-H, [4Fe-4S] ferredoxin subunit (NCBI), at 2,835,600 to 2,836,127 hydN b2714: ascG - DNA-binding transcriptional repressor (NCBI), at 2,836,276 to 2,837,286 ascG Position (kb) 2835 2836 2837Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2834.689 kb on - strand, within hypFat 2834.832 kb on - strand, within hypFat 2834.862 kb on + strand, within hypFat 2834.878 kb on - strand, within hypFat 2834.951 kb on - strand, within hypFat 2834.951 kb on - strand, within hypFat 2834.955 kb on + strand, within hypFat 2834.972 kb on + strand, within hypFat 2835.007 kb on + strand, within hypFat 2835.007 kb on + strand, within hypFat 2835.051 kb on + strand, within hypFat 2835.051 kb on + strand, within hypFat 2835.172 kb on - strand, within hypFat 2835.172 kb on - strand, within hypFat 2835.235 kb on - strandat 2835.264 kb on - strandat 2835.306 kb on + strandat 2835.415 kb on + strandat 2835.522 kb on - strandat 2835.522 kb on - strandat 2835.729 kb on - strand, within hydNat 2835.731 kb on - strand, within hydNat 2835.812 kb on - strand, within hydNat 2835.849 kb on - strand, within hydNat 2835.849 kb on - strand, within hydNat 2835.902 kb on + strand, within hydNat 2835.933 kb on + strand, within hydNat 2835.982 kb on + strand, within hydNat 2836.033 kb on - strand, within hydNat 2836.033 kb on - strand, within hydNat 2836.043 kb on - strand, within hydNat 2836.043 kb on - strand, within hydNat 2836.078 kb on - strandat 2836.436 kb on + strand, within ascGat 2836.444 kb on - strand, within ascGat 2836.444 kb on - strand, within ascGat 2836.444 kb on - strand, within ascGat 2836.516 kb on - strand, within ascGat 2836.653 kb on + strand, within ascGat 2836.653 kb on + strand, within ascGat 2836.653 kb on + strand, within ascGat 2836.653 kb on + strand, within ascGat 2836.687 kb on - strand, within ascGat 2836.754 kb on - strand, within ascGat 2836.760 kb on + strand, within ascGat 2836.768 kb on - strand, within ascGat 2836.768 kb on - strand, within ascGat 2836.802 kb on - strand, within ascGat 2836.802 kb on - strand, within ascGat 2836.810 kb on + strand, within ascGat 2836.877 kb on - strand, within ascGat 2837.055 kb on - strandat 2837.099 kb on - strand, within ascGat 2837.099 kb on - strand, within ascG

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol and Rhamnolipid (C)
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2,834,689 - hypF b2712 0.66 +0.2
2,834,832 - hypF b2712 0.73 +0.3
2,834,862 + hypF b2712 0.74 +0.1
2,834,878 - hypF b2712 0.75 +0.6
2,834,951 - hypF b2712 0.78 -0.4
2,834,951 - hypF b2712 0.78 +0.4
2,834,955 + hypF b2712 0.78 +0.0
2,834,972 + hypF b2712 0.79 +0.7
2,835,007 + hypF b2712 0.80 +1.3
2,835,007 + hypF b2712 0.80 +1.0
2,835,051 + hypF b2712 0.82 +0.6
2,835,051 + hypF b2712 0.82 +2.0
2,835,172 - hypF b2712 0.88 -1.3
2,835,172 - hypF b2712 0.88 +1.3
2,835,235 - +0.1
2,835,264 - +0.1
2,835,306 + -2.4
2,835,415 + +0.1
2,835,522 - +0.8
2,835,522 - -0.3
2,835,729 - hydN b2713 0.24 -0.4
2,835,731 - hydN b2713 0.25 -0.9
2,835,812 - hydN b2713 0.40 -0.5
2,835,849 - hydN b2713 0.47 +0.9
2,835,849 - hydN b2713 0.47 -0.4
2,835,902 + hydN b2713 0.57 -0.2
2,835,933 + hydN b2713 0.63 -0.5
2,835,982 + hydN b2713 0.72 +0.1
2,836,033 - hydN b2713 0.82 -1.0
2,836,033 - hydN b2713 0.82 +0.1
2,836,043 - hydN b2713 0.84 -0.9
2,836,043 - hydN b2713 0.84 -1.8
2,836,078 - +0.7
2,836,436 + ascG b2714 0.16 -0.3
2,836,444 - ascG b2714 0.17 +1.0
2,836,444 - ascG b2714 0.17 -3.3
2,836,444 - ascG b2714 0.17 -1.1
2,836,516 - ascG b2714 0.24 -0.5
2,836,653 + ascG b2714 0.37 -0.3
2,836,653 + ascG b2714 0.37 -0.7
2,836,653 + ascG b2714 0.37 -0.2
2,836,653 + ascG b2714 0.37 -2.1
2,836,687 - ascG b2714 0.41 +0.3
2,836,754 - ascG b2714 0.47 +0.6
2,836,760 + ascG b2714 0.48 +1.8
2,836,768 - ascG b2714 0.49 +2.0
2,836,768 - ascG b2714 0.49 -1.4
2,836,802 - ascG b2714 0.52 +0.4
2,836,802 - ascG b2714 0.52 -0.4
2,836,810 + ascG b2714 0.53 +0.1
2,836,877 - ascG b2714 0.59 +0.1
2,837,055 - -0.3
2,837,099 - ascG b2714 0.81 +1.2
2,837,099 - ascG b2714 0.81 +0.7

Or see this region's nucleotide sequence