Strain Fitness in Escherichia coli BW25113 around b3705

Experiment: Dodecanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpmH and rnpA are separated by 16 nucleotidesrnpA and yidD overlap by 37 nucleotidesyidD and yidC are separated by 2 nucleotidesyidC and trmE are separated by 105 nucleotides b3703: rpmH - 50S ribosomal protein L34 (NCBI), at 3,882,359 to 3,882,499 rpmH b3704: rnpA - ribonuclease P (NCBI), at 3,882,516 to 3,882,875 rnpA b4557: yidD - hypothetical protein (NCBI), at 3,882,839 to 3,883,096 yidD b3705: yidC - inner membrane protein translocase component YidC (NCBI), at 3,883,099 to 3,884,745 yidC b3706: trmE - tRNA modification GTPase (NCBI), at 3,884,851 to 3,886,215 trmE Position (kb) 3883 3884 3885Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3882.110 kb on + strandat 3882.110 kb on + strandat 3882.118 kb on - strandat 3882.118 kb on - strandat 3882.120 kb on - strandat 3882.163 kb on + strandat 3882.172 kb on - strandat 3882.876 kb on - strand, within yidDat 3882.876 kb on - strand, within yidDat 3882.883 kb on + strand, within yidDat 3882.901 kb on + strand, within yidDat 3882.901 kb on - strand, within yidDat 3882.902 kb on - strand, within yidDat 3882.941 kb on - strand, within yidDat 3882.960 kb on + strand, within yidDat 3882.978 kb on + strand, within yidDat 3883.034 kb on + strand, within yidDat 3883.034 kb on + strand, within yidDat 3884.716 kb on + strandat 3884.754 kb on + strandat 3884.777 kb on + strandat 3884.777 kb on + strandat 3884.817 kb on - strandat 3884.817 kb on - strandat 3884.817 kb on - strandat 3884.855 kb on - strandat 3884.855 kb on - strandat 3884.869 kb on + strandat 3884.869 kb on + strandat 3884.869 kb on + strandat 3884.869 kb on + strandat 3884.879 kb on + strandat 3884.883 kb on - strandat 3884.888 kb on - strandat 3884.897 kb on - strandat 3884.903 kb on + strandat 3884.937 kb on - strandat 3884.961 kb on + strandat 3884.961 kb on + strandat 3885.010 kb on + strand, within trmEat 3885.014 kb on + strand, within trmEat 3885.041 kb on - strand, within trmEat 3885.051 kb on - strand, within trmEat 3885.066 kb on - strand, within trmEat 3885.091 kb on + strand, within trmEat 3885.099 kb on - strand, within trmEat 3885.129 kb on + strand, within trmEat 3885.181 kb on - strand, within trmEat 3885.181 kb on - strand, within trmEat 3885.342 kb on - strand, within trmEat 3885.342 kb on - strand, within trmEat 3885.342 kb on - strand, within trmEat 3885.400 kb on - strand, within trmEat 3885.461 kb on + strand, within trmEat 3885.480 kb on - strand, within trmEat 3885.482 kb on - strand, within trmEat 3885.490 kb on + strand, within trmEat 3885.522 kb on + strand, within trmEat 3885.689 kb on + strand, within trmEat 3885.699 kb on - strand, within trmE

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol (C)
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3,882,110 + +0.7
3,882,110 + +1.3
3,882,118 - +1.4
3,882,118 - +0.2
3,882,120 - +0.7
3,882,163 + +0.5
3,882,172 - -0.1
3,882,876 - yidD b4557 0.14 -0.6
3,882,876 - yidD b4557 0.14 -1.6
3,882,883 + yidD b4557 0.17 -2.1
3,882,901 + yidD b4557 0.24 -0.8
3,882,901 - yidD b4557 0.24 -3.1
3,882,902 - yidD b4557 0.24 -1.0
3,882,941 - yidD b4557 0.40 -0.9
3,882,960 + yidD b4557 0.47 -1.5
3,882,978 + yidD b4557 0.54 +0.5
3,883,034 + yidD b4557 0.76 -2.0
3,883,034 + yidD b4557 0.76 +0.5
3,884,716 + -1.0
3,884,754 + -0.2
3,884,777 + +0.0
3,884,777 + -0.0
3,884,817 - -0.2
3,884,817 - -1.2
3,884,817 - -0.3
3,884,855 - -1.4
3,884,855 - -0.6
3,884,869 + -1.9
3,884,869 + -3.9
3,884,869 + -2.8
3,884,869 + -0.8
3,884,879 + -3.0
3,884,883 - +0.9
3,884,888 - -2.1
3,884,897 - -3.3
3,884,903 + -0.8
3,884,937 - -0.1
3,884,961 + -1.8
3,884,961 + -1.7
3,885,010 + trmE b3706 0.12 -2.8
3,885,014 + trmE b3706 0.12 -2.2
3,885,041 - trmE b3706 0.14 -1.0
3,885,051 - trmE b3706 0.15 -1.5
3,885,066 - trmE b3706 0.16 -1.4
3,885,091 + trmE b3706 0.18 -2.7
3,885,099 - trmE b3706 0.18 -2.5
3,885,129 + trmE b3706 0.20 -2.1
3,885,181 - trmE b3706 0.24 -1.9
3,885,181 - trmE b3706 0.24 -1.9
3,885,342 - trmE b3706 0.36 -2.1
3,885,342 - trmE b3706 0.36 -1.8
3,885,342 - trmE b3706 0.36 -1.1
3,885,400 - trmE b3706 0.40 +1.7
3,885,461 + trmE b3706 0.45 -2.0
3,885,480 - trmE b3706 0.46 -2.8
3,885,482 - trmE b3706 0.46 -1.7
3,885,490 + trmE b3706 0.47 -2.6
3,885,522 + trmE b3706 0.49 +0.1
3,885,689 + trmE b3706 0.61 -2.2
3,885,699 - trmE b3706 0.62 -1.2

Or see this region's nucleotide sequence