Strain Fitness in Escherichia coli BW25113 around b1928

Experiment: Dodecanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntamyA and yedD are separated by 33 nucleotidesyedD and yedE are separated by 186 nucleotides b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667 amyA b1928: yedD - hypothetical protein (NCBI), at 2,005,701 to 2,006,114 yedD b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506 yedE Position (kb) 2005 2006 2007Strain fitness (log2 ratio) -3 -2 -1 0 1at 2004.739 kb on - strand, within amyAat 2004.809 kb on + strand, within amyAat 2004.861 kb on - strand, within amyAat 2004.942 kb on - strand, within amyAat 2005.017 kb on - strand, within amyAat 2005.185 kb on + strand, within amyAat 2005.185 kb on + strand, within amyAat 2005.308 kb on + strand, within amyAat 2005.308 kb on + strand, within amyAat 2005.310 kb on + strand, within amyAat 2005.410 kb on - strand, within amyAat 2005.410 kb on - strand, within amyAat 2005.443 kb on - strand, within amyAat 2005.591 kb on - strandat 2005.633 kb on + strandat 2005.633 kb on + strandat 2005.659 kb on - strandat 2005.659 kb on - strandat 2005.729 kb on + strandat 2005.787 kb on - strand, within yedDat 2005.788 kb on + strand, within yedDat 2005.801 kb on - strand, within yedDat 2005.801 kb on - strand, within yedDat 2005.809 kb on - strand, within yedDat 2005.827 kb on - strand, within yedDat 2005.953 kb on + strand, within yedDat 2005.969 kb on - strand, within yedDat 2006.256 kb on - strandat 2006.284 kb on + strandat 2006.393 kb on - strandat 2006.393 kb on - strandat 2006.417 kb on + strandat 2006.417 kb on + strandat 2006.457 kb on - strand, within yedEat 2006.508 kb on + strand, within yedEat 2006.605 kb on - strand, within yedEat 2006.732 kb on - strand, within yedEat 2006.767 kb on - strand, within yedEat 2006.772 kb on - strand, within yedEat 2006.951 kb on - strand, within yedEat 2006.989 kb on + strand, within yedEat 2007.062 kb on + strand, within yedEat 2007.062 kb on + strand, within yedE

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol (C)
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2,004,739 - amyA b1927 0.38 -0.9
2,004,809 + amyA b1927 0.42 +0.2
2,004,861 - amyA b1927 0.46 +0.6
2,004,942 - amyA b1927 0.51 +0.4
2,005,017 - amyA b1927 0.56 +1.1
2,005,185 + amyA b1927 0.68 -0.9
2,005,185 + amyA b1927 0.68 -0.2
2,005,308 + amyA b1927 0.76 -0.2
2,005,308 + amyA b1927 0.76 +0.5
2,005,310 + amyA b1927 0.76 -0.7
2,005,410 - amyA b1927 0.83 -0.1
2,005,410 - amyA b1927 0.83 -0.9
2,005,443 - amyA b1927 0.85 +0.6
2,005,591 - +0.3
2,005,633 + -0.3
2,005,633 + -2.8
2,005,659 - -0.6
2,005,659 - -0.8
2,005,729 + +0.4
2,005,787 - yedD b1928 0.21 -0.9
2,005,788 + yedD b1928 0.21 -0.2
2,005,801 - yedD b1928 0.24 -2.7
2,005,801 - yedD b1928 0.24 -0.1
2,005,809 - yedD b1928 0.26 -0.5
2,005,827 - yedD b1928 0.30 -1.4
2,005,953 + yedD b1928 0.61 -0.6
2,005,969 - yedD b1928 0.65 -0.9
2,006,256 - +0.2
2,006,284 + -0.6
2,006,393 - +0.4
2,006,393 - -0.5
2,006,417 + +0.3
2,006,417 + +0.1
2,006,457 - yedE b1929 0.13 +0.2
2,006,508 + yedE b1929 0.17 +0.1
2,006,605 - yedE b1929 0.25 -0.7
2,006,732 - yedE b1929 0.36 -0.3
2,006,767 - yedE b1929 0.39 -0.1
2,006,772 - yedE b1929 0.39 -1.7
2,006,951 - yedE b1929 0.54 -0.4
2,006,989 + yedE b1929 0.57 +0.5
2,007,062 + yedE b1929 0.63 +0.7
2,007,062 + yedE b1929 0.63 +0.1

Or see this region's nucleotide sequence