Strain Fitness in Escherichia coli BW25113 around b1745

Experiment: Dodecanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntastE and astB overlap by 8 nucleotidesastB and astD overlap by 4 nucleotides b1744: astE - succinylglutamate desuccinylase (NCBI), at 1,823,979 to 1,824,947 astE b1745: astB - succinylarginine dihydrolase (NCBI), at 1,824,940 to 1,826,283 astB b1746: astD - succinylglutamic semialdehyde dehydrogenase (NCBI), at 1,826,280 to 1,827,758 astD Position (kb) 1824 1825 1826 1827Strain fitness (log2 ratio) -2 -1 0 1at 1824.017 kb on - strandat 1824.017 kb on - strandat 1824.032 kb on + strandat 1824.047 kb on - strandat 1824.047 kb on - strandat 1824.052 kb on - strandat 1824.053 kb on - strandat 1824.059 kb on - strandat 1824.059 kb on - strandat 1824.087 kb on + strand, within astEat 1824.090 kb on - strand, within astEat 1824.090 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.330 kb on + strand, within astEat 1824.333 kb on - strand, within astEat 1824.380 kb on + strand, within astEat 1824.397 kb on + strand, within astEat 1824.512 kb on + strand, within astEat 1824.650 kb on - strand, within astEat 1824.857 kb on + strandat 1824.871 kb on - strandat 1825.089 kb on - strand, within astBat 1825.285 kb on + strand, within astBat 1825.285 kb on + strand, within astBat 1825.293 kb on - strand, within astBat 1825.321 kb on - strand, within astBat 1825.321 kb on - strand, within astBat 1825.378 kb on + strand, within astBat 1825.727 kb on - strand, within astBat 1825.767 kb on - strand, within astBat 1826.034 kb on + strand, within astBat 1826.113 kb on - strand, within astBat 1826.358 kb on + strandat 1826.451 kb on - strand, within astDat 1826.451 kb on - strand, within astDat 1826.451 kb on - strand, within astDat 1826.614 kb on + strand, within astDat 1826.707 kb on + strand, within astDat 1826.730 kb on - strand, within astDat 1826.730 kb on - strand, within astDat 1826.786 kb on + strand, within astDat 1826.848 kb on - strand, within astDat 1826.848 kb on - strand, within astDat 1826.870 kb on - strand, within astDat 1826.949 kb on - strand, within astDat 1826.949 kb on - strand, within astDat 1827.115 kb on + strand, within astDat 1827.262 kb on - strand, within astDat 1827.262 kb on - strand, within astD

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol (C)
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1,824,017 - +0.7
1,824,017 - -0.4
1,824,032 + -1.4
1,824,047 - -1.7
1,824,047 - +0.2
1,824,052 - -1.2
1,824,053 - +0.2
1,824,059 - -0.5
1,824,059 - -1.5
1,824,087 + astE b1744 0.11 +0.5
1,824,090 - astE b1744 0.11 -0.6
1,824,090 - astE b1744 0.11 -0.6
1,824,095 - astE b1744 0.12 +0.0
1,824,095 - astE b1744 0.12 -1.2
1,824,095 - astE b1744 0.12 -2.1
1,824,095 - astE b1744 0.12 +0.0
1,824,330 + astE b1744 0.36 -0.7
1,824,333 - astE b1744 0.37 -1.2
1,824,380 + astE b1744 0.41 +0.4
1,824,397 + astE b1744 0.43 -0.0
1,824,512 + astE b1744 0.55 -0.2
1,824,650 - astE b1744 0.69 -1.1
1,824,857 + -0.3
1,824,871 - -0.1
1,825,089 - astB b1745 0.11 -0.5
1,825,285 + astB b1745 0.26 -0.6
1,825,285 + astB b1745 0.26 -0.5
1,825,293 - astB b1745 0.26 -0.5
1,825,321 - astB b1745 0.28 +0.1
1,825,321 - astB b1745 0.28 -0.4
1,825,378 + astB b1745 0.33 -1.6
1,825,727 - astB b1745 0.59 -0.9
1,825,767 - astB b1745 0.62 +0.0
1,826,034 + astB b1745 0.81 -0.3
1,826,113 - astB b1745 0.87 -0.3
1,826,358 + +0.4
1,826,451 - astD b1746 0.12 +0.0
1,826,451 - astD b1746 0.12 +0.1
1,826,451 - astD b1746 0.12 -0.8
1,826,614 + astD b1746 0.23 +0.1
1,826,707 + astD b1746 0.29 +0.6
1,826,730 - astD b1746 0.30 +0.7
1,826,730 - astD b1746 0.30 -0.4
1,826,786 + astD b1746 0.34 -1.5
1,826,848 - astD b1746 0.38 -0.7
1,826,848 - astD b1746 0.38 +0.0
1,826,870 - astD b1746 0.40 -0.7
1,826,949 - astD b1746 0.45 +0.5
1,826,949 - astD b1746 0.45 -0.9
1,827,115 + astD b1746 0.56 -0.6
1,827,262 - astD b1746 0.66 +0.4
1,827,262 - astD b1746 0.66 -0.6

Or see this region's nucleotide sequence