Experiment: Dodecanol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1191 and ldcA are separated by 94 nucleotides ldcA and mltE are separated by 99 nucleotides mltE and ycgR are separated by 1 nucleotides
b1191: b1191 - orf, hypothetical protein (VIMSS), at 1,239,558 to 1,241,294
b1191
b1192: ldcA - L,D-carboxypeptidase A (NCBI), at 1,241,389 to 1,242,303
ldcA
b1193: mltE - murein transglycosylase E (VIMSS), at 1,242,403 to 1,243,014
mltE
b1194: ycgR - protein involved in flagellar function (NCBI), at 1,243,016 to 1,243,750
ycgR
Position (kb)
1241
1242
1243 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1240.433 kb on + strand, within b1191 at 1240.506 kb on + strand, within b1191 at 1240.552 kb on - strand, within b1191 at 1240.627 kb on - strand, within b1191 at 1240.675 kb on - strand, within b1191 at 1240.750 kb on - strand, within b1191 at 1240.974 kb on + strand, within b1191 at 1240.995 kb on - strand, within b1191 at 1240.997 kb on - strand, within b1191 at 1241.032 kb on - strand, within b1191 at 1241.068 kb on + strand, within b1191 at 1241.102 kb on - strand, within b1191 at 1241.102 kb on - strand, within b1191 at 1241.112 kb on + strand, within b1191 at 1241.143 kb on - strand at 1241.159 kb on - strand at 1241.159 kb on - strand at 1241.231 kb on + strand at 1241.231 kb on + strand at 1241.331 kb on + strand at 1241.331 kb on + strand at 1241.424 kb on + strand at 1241.425 kb on - strand at 1241.433 kb on + strand at 1241.501 kb on + strand, within ldcA at 1241.572 kb on - strand, within ldcA at 1241.581 kb on + strand, within ldcA at 1241.747 kb on + strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.936 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.961 kb on - strand, within ldcA at 1241.999 kb on - strand, within ldcA at 1242.033 kb on + strand, within ldcA at 1242.208 kb on - strand, within ldcA at 1242.237 kb on - strand at 1242.284 kb on + strand at 1242.305 kb on - strand at 1242.337 kb on + strand at 1242.355 kb on - strand at 1242.365 kb on - strand at 1242.365 kb on - strand at 1242.465 kb on + strand, within mltE at 1242.655 kb on + strand, within mltE at 1243.159 kb on + strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.291 kb on - strand, within ycgR
Per-strain Table
Position Strand Gene LocusTag Fraction Dodecanol (C) remove 1,240,433 + b1191 b1191 0.50 +0.3 1,240,506 + b1191 b1191 0.55 -0.4 1,240,552 - b1191 b1191 0.57 +0.2 1,240,627 - b1191 b1191 0.62 +0.2 1,240,675 - b1191 b1191 0.64 +0.5 1,240,750 - b1191 b1191 0.69 +3.3 1,240,974 + b1191 b1191 0.82 -0.1 1,240,995 - b1191 b1191 0.83 -0.1 1,240,997 - b1191 b1191 0.83 +0.4 1,241,032 - b1191 b1191 0.85 +0.6 1,241,068 + b1191 b1191 0.87 -0.5 1,241,102 - b1191 b1191 0.89 -0.6 1,241,102 - b1191 b1191 0.89 +0.9 1,241,112 + b1191 b1191 0.89 +1.2 1,241,143 - -0.5 1,241,159 - -0.1 1,241,159 - +0.6 1,241,231 + +1.1 1,241,231 + -0.7 1,241,331 + +0.1 1,241,331 + +0.6 1,241,424 + -2.3 1,241,425 - -3.7 1,241,433 + -4.2 1,241,501 + ldcA b1192 0.12 -3.1 1,241,572 - ldcA b1192 0.20 -1.2 1,241,581 + ldcA b1192 0.21 -2.9 1,241,747 + ldcA b1192 0.39 -1.7 1,241,936 - ldcA b1192 0.60 -3.5 1,241,936 - ldcA b1192 0.60 -2.9 1,241,961 - ldcA b1192 0.63 -1.3 1,241,961 - ldcA b1192 0.63 -0.7 1,241,999 - ldcA b1192 0.67 -2.7 1,242,033 + ldcA b1192 0.70 -2.3 1,242,208 - ldcA b1192 0.90 -0.3 1,242,237 - -2.3 1,242,284 + -2.4 1,242,305 - -1.1 1,242,337 + -2.8 1,242,355 - -0.6 1,242,365 - +0.1 1,242,365 - -0.2 1,242,465 + mltE b1193 0.10 -2.8 1,242,655 + mltE b1193 0.41 +0.5 1,243,159 + ycgR b1194 0.19 -0.5 1,243,291 - ycgR b1194 0.37 +0.2 1,243,291 - ycgR b1194 0.37 +1.0
Or see this region's nucleotide sequence