Strain Fitness in Escherichia coli BW25113 around b1127

Experiment: Dodecanol (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpotA and pepT are separated by 249 nucleotidespepT and ycfD are separated by 48 nucleotides b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817 potA b1127: pepT - peptidase T (NCBI), at 1,185,067 to 1,186,293 pepT b1128: ycfD - orf, hypothetical protein (VIMSS), at 1,186,342 to 1,187,463 ycfD Position (kb) 1185 1186 1187Strain fitness (log2 ratio) -1 0 1at 1184.139 kb on - strand, within potAat 1184.163 kb on + strand, within potAat 1184.163 kb on + strand, within potAat 1184.286 kb on + strand, within potAat 1184.293 kb on + strand, within potAat 1184.334 kb on - strand, within potAat 1184.361 kb on - strand, within potAat 1184.599 kb on - strand, within potAat 1184.666 kb on + strand, within potAat 1184.692 kb on + strand, within potAat 1184.712 kb on + strandat 1184.724 kb on + strandat 1184.732 kb on - strandat 1184.847 kb on + strandat 1185.128 kb on + strandat 1185.135 kb on - strandat 1185.135 kb on - strandat 1185.153 kb on + strandat 1185.190 kb on + strand, within pepTat 1185.276 kb on + strand, within pepTat 1185.296 kb on + strand, within pepTat 1185.409 kb on + strand, within pepTat 1185.639 kb on + strand, within pepTat 1185.647 kb on + strand, within pepTat 1185.720 kb on + strand, within pepTat 1185.746 kb on - strand, within pepTat 1185.779 kb on - strand, within pepTat 1185.886 kb on + strand, within pepTat 1185.986 kb on - strand, within pepTat 1186.009 kb on + strand, within pepTat 1186.182 kb on - strandat 1186.468 kb on - strand, within ycfDat 1186.507 kb on - strand, within ycfDat 1186.533 kb on - strand, within ycfDat 1186.778 kb on + strand, within ycfDat 1186.778 kb on + strand, within ycfDat 1186.805 kb on - strand, within ycfDat 1186.876 kb on - strand, within ycfDat 1186.942 kb on - strand, within ycfDat 1186.950 kb on + strand, within ycfDat 1186.950 kb on + strand, within ycfDat 1187.202 kb on + strand, within ycfDat 1187.292 kb on + strand, within ycfDat 1187.292 kb on + strand, within ycfD

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecanol (C)
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1,184,139 - potA b1126 0.40 -0.6
1,184,163 + potA b1126 0.42 -0.2
1,184,163 + potA b1126 0.42 +0.2
1,184,286 + potA b1126 0.53 -0.0
1,184,293 + potA b1126 0.54 +0.1
1,184,334 - potA b1126 0.57 +0.4
1,184,361 - potA b1126 0.60 +0.5
1,184,599 - potA b1126 0.81 -0.5
1,184,666 + potA b1126 0.87 +0.1
1,184,692 + potA b1126 0.89 -1.5
1,184,712 + +0.4
1,184,724 + +0.1
1,184,732 - +0.5
1,184,847 + +1.2
1,185,128 + -0.3
1,185,135 - -0.1
1,185,135 - +0.8
1,185,153 + +0.1
1,185,190 + pepT b1127 0.10 +1.1
1,185,276 + pepT b1127 0.17 -0.2
1,185,296 + pepT b1127 0.19 +0.2
1,185,409 + pepT b1127 0.28 -0.5
1,185,639 + pepT b1127 0.47 +0.6
1,185,647 + pepT b1127 0.47 +0.9
1,185,720 + pepT b1127 0.53 -0.2
1,185,746 - pepT b1127 0.55 -0.5
1,185,779 - pepT b1127 0.58 +0.8
1,185,886 + pepT b1127 0.67 +0.7
1,185,986 - pepT b1127 0.75 -0.8
1,186,009 + pepT b1127 0.77 +0.0
1,186,182 - -0.7
1,186,468 - ycfD b1128 0.11 +0.8
1,186,507 - ycfD b1128 0.15 +0.4
1,186,533 - ycfD b1128 0.17 +0.0
1,186,778 + ycfD b1128 0.39 +0.4
1,186,778 + ycfD b1128 0.39 -0.1
1,186,805 - ycfD b1128 0.41 +0.0
1,186,876 - ycfD b1128 0.48 -0.4
1,186,942 - ycfD b1128 0.53 -0.1
1,186,950 + ycfD b1128 0.54 +0.5
1,186,950 + ycfD b1128 0.54 +0.6
1,187,202 + ycfD b1128 0.77 -0.6
1,187,292 + ycfD b1128 0.85 +0.2
1,187,292 + ycfD b1128 0.85 +0.1

Or see this region's nucleotide sequence