Experiment: KB with Solanesol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3617 and Psyr_3618 are separated by 102 nucleotides Psyr_3618 and Psyr_3619 are separated by 90 nucleotides Psyr_3619 and Psyr_3620 are separated by 69 nucleotides Psyr_3620 and Psyr_3621 are separated by 135 nucleotides
Psyr_3617: Psyr_3617 - Thiopurine S-methyltransferase, at 4,301,417 to 4,302,079
_3617
Psyr_3618: Psyr_3618 - Catalytic LigB subunit of aromatic ring-opening dioxygenase, at 4,302,182 to 4,302,949
_3618
Psyr_3619: Psyr_3619 - Helicase, C-terminal:DbpA, RNA-binding:DEAD/DEAH box helicase, N-terminal, at 4,303,040 to 4,304,713
_3619
Psyr_3620: Psyr_3620 - hypothetical protein, at 4,304,783 to 4,305,031
_3620
Psyr_3621: Psyr_3621 - conserved hypothetical protein, at 4,305,167 to 4,305,928
_3621
Position (kb) 4303
4304
4305 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4302.120 kb on - strand at 4302.120 kb on - strand at 4302.151 kb on + strand at 4302.152 kb on - strand at 4302.167 kb on - strand at 4302.179 kb on + strand at 4302.179 kb on + strand at 4302.179 kb on + strand at 4302.179 kb on + strand at 4302.180 kb on - strand at 4302.180 kb on - strand at 4302.180 kb on - strand at 4302.180 kb on - strand at 4302.180 kb on - strand at 4302.199 kb on + strand at 4302.199 kb on + strand at 4302.199 kb on + strand at 4302.200 kb on - strand at 4302.645 kb on + strand, within Psyr_3618 at 4302.646 kb on - strand, within Psyr_3618 at 4302.677 kb on - strand, within Psyr_3618 at 4302.858 kb on - strand, within Psyr_3618 at 4302.931 kb on + strand at 4302.931 kb on + strand at 4302.931 kb on + strand at 4302.932 kb on - strand at 4302.932 kb on - strand at 4302.935 kb on + strand at 4303.238 kb on - strand, within Psyr_3619 at 4303.429 kb on + strand, within Psyr_3619 at 4304.846 kb on - strand, within Psyr_3620 at 4304.895 kb on - strand, within Psyr_3620 at 4304.931 kb on - strand, within Psyr_3620 at 4304.931 kb on - strand, within Psyr_3620 at 4304.942 kb on - strand, within Psyr_3620 at 4305.077 kb on + strand at 4305.164 kb on + strand at 4305.164 kb on + strand at 4305.164 kb on + strand at 4305.165 kb on - strand at 4305.330 kb on + strand, within Psyr_3621 at 4305.331 kb on - strand, within Psyr_3621 at 4305.492 kb on + strand, within Psyr_3621 at 4305.492 kb on + strand, within Psyr_3621 at 4305.493 kb on - strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.564 kb on + strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621 at 4305.565 kb on - strand, within Psyr_3621
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Solanesol 10 mM remove 4,302,120 - +4.0 4,302,120 - +1.7 4,302,151 + +2.2 4,302,152 - -0.9 4,302,167 - -0.3 4,302,179 + -2.4 4,302,179 + -0.3 4,302,179 + +1.6 4,302,179 + -0.7 4,302,180 - +0.0 4,302,180 - +0.7 4,302,180 - +0.0 4,302,180 - +2.8 4,302,180 - +3.5 4,302,199 + +1.2 4,302,199 + -0.8 4,302,199 + -0.3 4,302,200 - -0.8 4,302,645 + Psyr_3618 0.60 +2.5 4,302,646 - Psyr_3618 0.60 +1.2 4,302,677 - Psyr_3618 0.64 -0.4 4,302,858 - Psyr_3618 0.88 +0.6 4,302,931 + -0.4 4,302,931 + +0.5 4,302,931 + -0.6 4,302,932 - -1.1 4,302,932 - +0.0 4,302,935 + +0.1 4,303,238 - Psyr_3619 0.12 -0.2 4,303,429 + Psyr_3619 0.23 -0.4 4,304,846 - Psyr_3620 0.25 +0.9 4,304,895 - Psyr_3620 0.45 -1.4 4,304,931 - Psyr_3620 0.59 -1.1 4,304,931 - Psyr_3620 0.59 +2.8 4,304,942 - Psyr_3620 0.64 +1.4 4,305,077 + -1.0 4,305,164 + -1.5 4,305,164 + +0.7 4,305,164 + +0.3 4,305,165 - -1.6 4,305,330 + Psyr_3621 0.21 -1.9 4,305,331 - Psyr_3621 0.22 +0.2 4,305,492 + Psyr_3621 0.43 -1.6 4,305,492 + Psyr_3621 0.43 -1.2 4,305,493 - Psyr_3621 0.43 +3.5 4,305,564 + Psyr_3621 0.52 +1.4 4,305,564 + Psyr_3621 0.52 -0.8 4,305,564 + Psyr_3621 0.52 +0.8 4,305,564 + Psyr_3621 0.52 +0.8 4,305,564 + Psyr_3621 0.52 +0.4 4,305,564 + Psyr_3621 0.52 +2.2 4,305,564 + Psyr_3621 0.52 -1.4 4,305,564 + Psyr_3621 0.52 +0.1 4,305,565 - Psyr_3621 0.52 +1.8 4,305,565 - Psyr_3621 0.52 -0.7 4,305,565 - Psyr_3621 0.52 +1.0 4,305,565 - Psyr_3621 0.52 -1.4 4,305,565 - Psyr_3621 0.52 -0.7 4,305,565 - Psyr_3621 0.52 -0.9 4,305,565 - Psyr_3621 0.52 -0.6 4,305,565 - Psyr_3621 0.52 +0.7 4,305,565 - Psyr_3621 0.52 -1.8 4,305,565 - Psyr_3621 0.52 +1.0 4,305,565 - Psyr_3621 0.52 +0.0 4,305,565 - Psyr_3621 0.52 +3.2 4,305,565 - Psyr_3621 0.52 +0.5 4,305,565 - Psyr_3621 0.52 -0.9 4,305,565 - Psyr_3621 0.52 +1.4 4,305,565 - Psyr_3621 0.52 -0.4
Or see this region's nucleotide sequence