Experiment: KB with Solanesol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1364 and Psyr_1365 are separated by 13 nucleotides Psyr_1365 and Psyr_1366 are separated by 7 nucleotides Psyr_1366 and Psyr_1367 are separated by 104 nucleotides
Psyr_1364: Psyr_1364 - cell division protein FtsB, at 1,548,383 to 1,548,739
_1364
Psyr_1365: Psyr_1365 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,548,753 to 1,549,463
_1365
Psyr_1366: Psyr_1366 - transcriptional regulator, LysR family, at 1,549,471 to 1,550,367
_1366
Psyr_1367: Psyr_1367 - Zinc-containing alcohol dehydrogenase superfamily, at 1,550,472 to 1,551,584
_1367
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1548.472 kb on + strand, within Psyr_1364 at 1548.737 kb on + strand at 1549.432 kb on + strand at 1549.482 kb on - strand at 1549.515 kb on - strand at 1549.646 kb on + strand, within Psyr_1366 at 1549.646 kb on + strand, within Psyr_1366 at 1549.647 kb on - strand, within Psyr_1366 at 1549.649 kb on + strand, within Psyr_1366 at 1549.666 kb on + strand, within Psyr_1366 at 1549.666 kb on + strand, within Psyr_1366 at 1549.713 kb on + strand, within Psyr_1366 at 1549.713 kb on + strand, within Psyr_1366 at 1549.713 kb on + strand, within Psyr_1366 at 1549.714 kb on - strand, within Psyr_1366 at 1549.850 kb on + strand, within Psyr_1366 at 1549.850 kb on + strand, within Psyr_1366 at 1549.850 kb on + strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.851 kb on - strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.871 kb on + strand, within Psyr_1366 at 1549.872 kb on - strand, within Psyr_1366 at 1549.872 kb on - strand, within Psyr_1366 at 1549.872 kb on - strand, within Psyr_1366 at 1550.009 kb on + strand, within Psyr_1366 at 1550.010 kb on - strand, within Psyr_1366 at 1550.214 kb on - strand, within Psyr_1366 at 1550.364 kb on - strand at 1550.364 kb on - strand at 1550.380 kb on + strand at 1550.381 kb on - strand at 1550.431 kb on - strand at 1550.433 kb on + strand at 1550.433 kb on + strand at 1550.433 kb on + strand at 1550.434 kb on - strand at 1550.575 kb on + strand at 1550.576 kb on - strand at 1550.578 kb on + strand at 1550.578 kb on + strand at 1550.578 kb on + strand at 1550.579 kb on - strand at 1550.607 kb on + strand, within Psyr_1367 at 1550.736 kb on + strand, within Psyr_1367 at 1550.817 kb on - strand, within Psyr_1367 at 1550.817 kb on - strand, within Psyr_1367 at 1550.817 kb on - strand, within Psyr_1367 at 1550.853 kb on + strand, within Psyr_1367 at 1550.877 kb on + strand, within Psyr_1367 at 1550.904 kb on + strand, within Psyr_1367 at 1550.905 kb on - strand, within Psyr_1367 at 1550.911 kb on + strand, within Psyr_1367 at 1550.912 kb on - strand, within Psyr_1367 at 1550.912 kb on - strand, within Psyr_1367 at 1550.912 kb on - strand, within Psyr_1367
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Solanesol 10 mM remove 1,548,472 + Psyr_1364 0.25 +0.2 1,548,737 + -0.4 1,549,432 + -0.1 1,549,482 - +0.3 1,549,515 - -0.7 1,549,646 + Psyr_1366 0.20 -0.5 1,549,646 + Psyr_1366 0.20 +0.6 1,549,647 - Psyr_1366 0.20 +0.3 1,549,649 + Psyr_1366 0.20 -0.0 1,549,666 + Psyr_1366 0.22 -0.8 1,549,666 + Psyr_1366 0.22 +0.7 1,549,713 + Psyr_1366 0.27 +0.9 1,549,713 + Psyr_1366 0.27 +0.3 1,549,713 + Psyr_1366 0.27 -0.6 1,549,714 - Psyr_1366 0.27 +0.2 1,549,850 + Psyr_1366 0.42 -0.2 1,549,850 + Psyr_1366 0.42 -0.8 1,549,850 + Psyr_1366 0.42 -0.3 1,549,851 - Psyr_1366 0.42 -0.7 1,549,851 - Psyr_1366 0.42 -0.5 1,549,851 - Psyr_1366 0.42 +2.2 1,549,851 - Psyr_1366 0.42 +1.5 1,549,851 - Psyr_1366 0.42 +0.1 1,549,871 + Psyr_1366 0.45 -0.2 1,549,871 + Psyr_1366 0.45 +0.8 1,549,871 + Psyr_1366 0.45 +1.2 1,549,871 + Psyr_1366 0.45 +1.3 1,549,871 + Psyr_1366 0.45 -1.2 1,549,871 + Psyr_1366 0.45 +0.9 1,549,871 + Psyr_1366 0.45 +2.2 1,549,872 - Psyr_1366 0.45 +1.2 1,549,872 - Psyr_1366 0.45 +2.2 1,549,872 - Psyr_1366 0.45 -0.0 1,550,009 + Psyr_1366 0.60 -0.6 1,550,010 - Psyr_1366 0.60 +0.6 1,550,214 - Psyr_1366 0.83 +0.8 1,550,364 - -1.1 1,550,364 - +3.1 1,550,380 + +0.8 1,550,381 - -0.7 1,550,431 - -1.0 1,550,433 + -0.5 1,550,433 + +0.7 1,550,433 + -0.1 1,550,434 - +0.3 1,550,575 + +4.0 1,550,576 - -2.7 1,550,578 + -1.1 1,550,578 + +0.5 1,550,578 + +0.9 1,550,579 - -1.8 1,550,607 + Psyr_1367 0.12 -0.7 1,550,736 + Psyr_1367 0.24 +0.9 1,550,817 - Psyr_1367 0.31 -2.8 1,550,817 - Psyr_1367 0.31 -0.5 1,550,817 - Psyr_1367 0.31 -2.1 1,550,853 + Psyr_1367 0.34 -0.4 1,550,877 + Psyr_1367 0.36 -0.3 1,550,904 + Psyr_1367 0.39 +0.6 1,550,905 - Psyr_1367 0.39 +0.6 1,550,911 + Psyr_1367 0.39 -1.0 1,550,912 - Psyr_1367 0.40 -1.1 1,550,912 - Psyr_1367 0.40 -1.3 1,550,912 - Psyr_1367 0.40 -0.4
Or see this region's nucleotide sequence