Experiment: KB with Biochanin a diacetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3429 and Psyr_3430 are separated by 75 nucleotides Psyr_3430 and Psyr_3431 are separated by 45 nucleotides Psyr_3431 and Psyr_3432 are separated by 6 nucleotides
Psyr_3429: Psyr_3429 - CheW-like protein, at 4,092,173 to 4,093,075
_3429
Psyr_3430: Psyr_3430 - Cobyrinic acid a,c-diamide synthase, at 4,093,151 to 4,093,939
_3430
Psyr_3431: Psyr_3431 - OmpA/MotB, at 4,093,985 to 4,094,872
_3431
Psyr_3432: Psyr_3432 - MotA/TolQ/ExbB proton channel, at 4,094,879 to 4,095,619
_3432
Position (kb)
4093
4094 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4092.174 kb on + strand at 4092.174 kb on + strand at 4092.175 kb on - strand at 4092.393 kb on + strand, within Psyr_3429 at 4092.393 kb on + strand, within Psyr_3429 at 4092.394 kb on - strand, within Psyr_3429 at 4092.825 kb on - strand, within Psyr_3429 at 4092.978 kb on + strand, within Psyr_3429 at 4093.028 kb on + strand at 4093.028 kb on + strand at 4093.028 kb on + strand at 4093.029 kb on - strand at 4093.034 kb on + strand at 4093.034 kb on + strand at 4093.035 kb on - strand at 4093.035 kb on - strand at 4093.035 kb on - strand at 4093.088 kb on - strand at 4093.088 kb on - strand at 4093.130 kb on + strand at 4093.130 kb on + strand at 4093.130 kb on + strand at 4093.130 kb on + strand at 4093.130 kb on + strand at 4093.131 kb on - strand at 4093.131 kb on - strand at 4093.186 kb on + strand at 4093.186 kb on + strand at 4093.187 kb on - strand at 4093.187 kb on - strand at 4093.187 kb on - strand at 4093.187 kb on - strand at 4093.187 kb on - strand at 4093.250 kb on - strand, within Psyr_3430 at 4093.296 kb on + strand, within Psyr_3430 at 4093.296 kb on + strand, within Psyr_3430 at 4093.296 kb on + strand, within Psyr_3430 at 4093.297 kb on - strand, within Psyr_3430 at 4093.297 kb on - strand, within Psyr_3430 at 4093.297 kb on - strand, within Psyr_3430 at 4093.297 kb on - strand, within Psyr_3430 at 4093.297 kb on - strand, within Psyr_3430 at 4093.297 kb on - strand, within Psyr_3430 at 4093.297 kb on - strand, within Psyr_3430 at 4093.395 kb on + strand, within Psyr_3430 at 4093.395 kb on + strand, within Psyr_3430 at 4093.396 kb on - strand, within Psyr_3430 at 4093.438 kb on + strand, within Psyr_3430 at 4093.567 kb on - strand, within Psyr_3430 at 4093.674 kb on - strand, within Psyr_3430 at 4093.684 kb on + strand, within Psyr_3430 at 4093.684 kb on + strand, within Psyr_3430 at 4093.684 kb on + strand, within Psyr_3430 at 4093.744 kb on + strand, within Psyr_3430 at 4093.789 kb on + strand, within Psyr_3430 at 4093.800 kb on + strand, within Psyr_3430 at 4093.800 kb on + strand, within Psyr_3430 at 4093.940 kb on + strand at 4093.941 kb on - strand at 4093.986 kb on + strand at 4094.156 kb on + strand, within Psyr_3431 at 4094.156 kb on + strand, within Psyr_3431 at 4094.329 kb on + strand, within Psyr_3431 at 4094.470 kb on + strand, within Psyr_3431 at 4094.594 kb on + strand, within Psyr_3431 at 4094.594 kb on + strand, within Psyr_3431 at 4094.739 kb on + strand, within Psyr_3431 at 4094.739 kb on + strand, within Psyr_3431 at 4094.739 kb on + strand, within Psyr_3431 at 4094.769 kb on + strand, within Psyr_3431 at 4094.769 kb on + strand, within Psyr_3431 at 4094.769 kb on + strand, within Psyr_3431 at 4094.770 kb on - strand, within Psyr_3431 at 4094.770 kb on - strand, within Psyr_3431 at 4094.796 kb on - strand at 4094.796 kb on - strand at 4094.800 kb on + strand at 4094.800 kb on + strand at 4094.801 kb on - strand at 4094.814 kb on + strand at 4094.814 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Biochanin a diacetate 10 mM remove 4,092,174 + +1.6 4,092,174 + +1.1 4,092,175 - +0.5 4,092,393 + Psyr_3429 0.24 -0.6 4,092,393 + Psyr_3429 0.24 -0.5 4,092,394 - Psyr_3429 0.24 +0.7 4,092,825 - Psyr_3429 0.72 -0.4 4,092,978 + Psyr_3429 0.89 +1.3 4,093,028 + -1.2 4,093,028 + +2.1 4,093,028 + +0.1 4,093,029 - -1.1 4,093,034 + -0.7 4,093,034 + -0.9 4,093,035 - -0.9 4,093,035 - +0.1 4,093,035 - +0.5 4,093,088 - +2.5 4,093,088 - -0.9 4,093,130 + -1.1 4,093,130 + +1.3 4,093,130 + +0.4 4,093,130 + +1.4 4,093,130 + -2.5 4,093,131 - +0.7 4,093,131 - +0.4 4,093,186 + -0.8 4,093,186 + +0.2 4,093,187 - +0.5 4,093,187 - -0.3 4,093,187 - +0.6 4,093,187 - -0.4 4,093,187 - -0.1 4,093,250 - Psyr_3430 0.13 -0.8 4,093,296 + Psyr_3430 0.18 -0.1 4,093,296 + Psyr_3430 0.18 -0.3 4,093,296 + Psyr_3430 0.18 +0.6 4,093,297 - Psyr_3430 0.19 -0.8 4,093,297 - Psyr_3430 0.19 -0.5 4,093,297 - Psyr_3430 0.19 -0.7 4,093,297 - Psyr_3430 0.19 +3.1 4,093,297 - Psyr_3430 0.19 +1.4 4,093,297 - Psyr_3430 0.19 -0.5 4,093,297 - Psyr_3430 0.19 +0.2 4,093,395 + Psyr_3430 0.31 -1.4 4,093,395 + Psyr_3430 0.31 +0.5 4,093,396 - Psyr_3430 0.31 -0.5 4,093,438 + Psyr_3430 0.36 -0.5 4,093,567 - Psyr_3430 0.53 +0.8 4,093,674 - Psyr_3430 0.66 -1.8 4,093,684 + Psyr_3430 0.68 -0.0 4,093,684 + Psyr_3430 0.68 -2.1 4,093,684 + Psyr_3430 0.68 -0.5 4,093,744 + Psyr_3430 0.75 -0.2 4,093,789 + Psyr_3430 0.81 -0.9 4,093,800 + Psyr_3430 0.82 -1.3 4,093,800 + Psyr_3430 0.82 -1.4 4,093,940 + +2.4 4,093,941 - +3.1 4,093,986 + -1.3 4,094,156 + Psyr_3431 0.19 -0.7 4,094,156 + Psyr_3431 0.19 +2.4 4,094,329 + Psyr_3431 0.39 +0.0 4,094,470 + Psyr_3431 0.55 -0.1 4,094,594 + Psyr_3431 0.69 -0.5 4,094,594 + Psyr_3431 0.69 -2.9 4,094,739 + Psyr_3431 0.85 -1.9 4,094,739 + Psyr_3431 0.85 +0.3 4,094,739 + Psyr_3431 0.85 +2.1 4,094,769 + Psyr_3431 0.88 +1.5 4,094,769 + Psyr_3431 0.88 +0.1 4,094,769 + Psyr_3431 0.88 +2.1 4,094,770 - Psyr_3431 0.88 +1.8 4,094,770 - Psyr_3431 0.88 +1.2 4,094,796 - +0.5 4,094,796 - -1.0 4,094,800 + +0.8 4,094,800 + +1.5 4,094,801 - -0.4 4,094,814 + -0.4 4,094,814 + -0.7
Or see this region's nucleotide sequence