Experiment: KB with Biochanin a diacetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3060 and Psyr_3061 are separated by 109 nucleotides Psyr_3061 and Psyr_3062 are separated by 42 nucleotides Psyr_3062 and Psyr_3063 are separated by 158 nucleotides
Psyr_3060: Psyr_3060 - GAF:ATP-binding region, ATPase-like:Histidine kinase A, N-terminal, at 3,659,376 to 3,660,704
_3060
Psyr_3061: Psyr_3061 - putative glutathione S-transferase, at 3,660,814 to 3,661,461
_3061
Psyr_3062: Psyr_3062 - transcriptional regulator, LysR family, at 3,661,504 to 3,662,400
_3062
Psyr_3063: Psyr_3063 - Major facilitator superfamily, at 3,662,559 to 3,664,124
_3063
Position (kb)
3661
3662
3663 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3660.643 kb on - strand at 3660.674 kb on + strand at 3660.691 kb on + strand at 3660.691 kb on + strand at 3660.692 kb on - strand at 3660.703 kb on - strand at 3660.853 kb on + strand at 3660.853 kb on + strand at 3661.149 kb on - strand, within Psyr_3061 at 3661.166 kb on + strand, within Psyr_3061 at 3661.167 kb on - strand, within Psyr_3061 at 3661.167 kb on - strand, within Psyr_3061 at 3661.167 kb on - strand, within Psyr_3061 at 3661.383 kb on + strand, within Psyr_3061 at 3661.383 kb on + strand, within Psyr_3061 at 3661.457 kb on + strand at 3661.457 kb on + strand at 3661.457 kb on + strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.461 kb on + strand at 3661.462 kb on - strand at 3661.622 kb on + strand, within Psyr_3062 at 3661.622 kb on + strand, within Psyr_3062 at 3661.737 kb on + strand, within Psyr_3062 at 3661.740 kb on + strand, within Psyr_3062 at 3661.740 kb on + strand, within Psyr_3062 at 3661.740 kb on + strand, within Psyr_3062 at 3661.741 kb on - strand, within Psyr_3062 at 3661.741 kb on - strand, within Psyr_3062 at 3661.878 kb on + strand, within Psyr_3062 at 3661.895 kb on + strand, within Psyr_3062 at 3661.896 kb on - strand, within Psyr_3062 at 3662.054 kb on + strand, within Psyr_3062 at 3662.058 kb on + strand, within Psyr_3062 at 3662.058 kb on + strand, within Psyr_3062 at 3662.058 kb on + strand, within Psyr_3062 at 3662.059 kb on - strand, within Psyr_3062 at 3662.292 kb on + strand, within Psyr_3062 at 3662.293 kb on - strand, within Psyr_3062 at 3662.293 kb on - strand, within Psyr_3062 at 3662.411 kb on + strand at 3662.432 kb on - strand at 3662.434 kb on - strand at 3662.434 kb on - strand at 3662.434 kb on - strand at 3662.434 kb on - strand at 3662.434 kb on - strand at 3662.446 kb on + strand at 3662.446 kb on + strand at 3662.446 kb on + strand at 3662.446 kb on + strand at 3662.446 kb on + strand at 3662.446 kb on + strand at 3662.446 kb on + strand at 3662.447 kb on - strand at 3662.447 kb on - strand at 3662.447 kb on - strand at 3662.447 kb on - strand at 3662.447 kb on - strand at 3662.447 kb on - strand at 3662.447 kb on - strand at 3662.447 kb on - strand at 3662.678 kb on - strand at 3662.739 kb on + strand, within Psyr_3063 at 3662.740 kb on - strand, within Psyr_3063 at 3662.740 kb on - strand, within Psyr_3063 at 3662.751 kb on + strand, within Psyr_3063 at 3662.752 kb on - strand, within Psyr_3063 at 3662.793 kb on + strand, within Psyr_3063 at 3662.793 kb on + strand, within Psyr_3063 at 3662.793 kb on + strand, within Psyr_3063 at 3662.794 kb on - strand, within Psyr_3063 at 3663.013 kb on + strand, within Psyr_3063 at 3663.013 kb on + strand, within Psyr_3063 at 3663.013 kb on + strand, within Psyr_3063 at 3663.013 kb on + strand, within Psyr_3063 at 3663.013 kb on + strand, within Psyr_3063 at 3663.014 kb on - strand, within Psyr_3063 at 3663.014 kb on - strand, within Psyr_3063 at 3663.014 kb on - strand, within Psyr_3063 at 3663.014 kb on - strand, within Psyr_3063 at 3663.014 kb on - strand, within Psyr_3063 at 3663.164 kb on + strand, within Psyr_3063 at 3663.216 kb on + strand, within Psyr_3063 at 3663.216 kb on + strand, within Psyr_3063 at 3663.217 kb on - strand, within Psyr_3063 at 3663.217 kb on - strand, within Psyr_3063 at 3663.217 kb on - strand, within Psyr_3063
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Biochanin a diacetate 10 mM remove 3,660,643 - -0.6 3,660,674 + +0.2 3,660,691 + +0.7 3,660,691 + -2.4 3,660,692 - -1.5 3,660,703 - -0.6 3,660,853 + -0.4 3,660,853 + +0.3 3,661,149 - Psyr_3061 0.52 +2.4 3,661,166 + Psyr_3061 0.54 +0.7 3,661,167 - Psyr_3061 0.54 +2.2 3,661,167 - Psyr_3061 0.54 +0.4 3,661,167 - Psyr_3061 0.54 +0.3 3,661,383 + Psyr_3061 0.88 +0.2 3,661,383 + Psyr_3061 0.88 -0.0 3,661,457 + +0.2 3,661,457 + -0.1 3,661,457 + +1.8 3,661,458 - +0.3 3,661,458 - -0.7 3,661,458 - +2.6 3,661,461 + -1.1 3,661,462 - +0.4 3,661,622 + Psyr_3062 0.13 +0.0 3,661,622 + Psyr_3062 0.13 +1.4 3,661,737 + Psyr_3062 0.26 +2.7 3,661,740 + Psyr_3062 0.26 -1.0 3,661,740 + Psyr_3062 0.26 -0.2 3,661,740 + Psyr_3062 0.26 +0.5 3,661,741 - Psyr_3062 0.26 +0.7 3,661,741 - Psyr_3062 0.26 +0.1 3,661,878 + Psyr_3062 0.42 -0.2 3,661,895 + Psyr_3062 0.44 -0.4 3,661,896 - Psyr_3062 0.44 -0.3 3,662,054 + Psyr_3062 0.61 -0.6 3,662,058 + Psyr_3062 0.62 +1.2 3,662,058 + Psyr_3062 0.62 -0.2 3,662,058 + Psyr_3062 0.62 -0.2 3,662,059 - Psyr_3062 0.62 -1.2 3,662,292 + Psyr_3062 0.88 -1.7 3,662,293 - Psyr_3062 0.88 -1.5 3,662,293 - Psyr_3062 0.88 +0.2 3,662,411 + -1.2 3,662,432 - -0.1 3,662,434 - -0.8 3,662,434 - -1.5 3,662,434 - +0.4 3,662,434 - +0.3 3,662,434 - -2.4 3,662,446 + +2.5 3,662,446 + +1.4 3,662,446 + -0.7 3,662,446 + -1.3 3,662,446 + +0.4 3,662,446 + +0.9 3,662,446 + -0.2 3,662,447 - +2.0 3,662,447 - -0.7 3,662,447 - -0.8 3,662,447 - +0.4 3,662,447 - -0.1 3,662,447 - -0.1 3,662,447 - -1.1 3,662,447 - -0.3 3,662,678 - +1.5 3,662,739 + Psyr_3063 0.11 +3.0 3,662,740 - Psyr_3063 0.12 -1.8 3,662,740 - Psyr_3063 0.12 +0.0 3,662,751 + Psyr_3063 0.12 -2.0 3,662,752 - Psyr_3063 0.12 -0.2 3,662,793 + Psyr_3063 0.15 +0.2 3,662,793 + Psyr_3063 0.15 -0.0 3,662,793 + Psyr_3063 0.15 -0.2 3,662,794 - Psyr_3063 0.15 -0.4 3,663,013 + Psyr_3063 0.29 -0.2 3,663,013 + Psyr_3063 0.29 -0.1 3,663,013 + Psyr_3063 0.29 -0.4 3,663,013 + Psyr_3063 0.29 +0.2 3,663,013 + Psyr_3063 0.29 +0.9 3,663,014 - Psyr_3063 0.29 -0.4 3,663,014 - Psyr_3063 0.29 +0.8 3,663,014 - Psyr_3063 0.29 +2.7 3,663,014 - Psyr_3063 0.29 +2.2 3,663,014 - Psyr_3063 0.29 -0.6 3,663,164 + Psyr_3063 0.39 -0.4 3,663,216 + Psyr_3063 0.42 -1.0 3,663,216 + Psyr_3063 0.42 +0.6 3,663,217 - Psyr_3063 0.42 +0.8 3,663,217 - Psyr_3063 0.42 -0.1 3,663,217 - Psyr_3063 0.42 +4.0
Or see this region's nucleotide sequence