Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_0999

Experiment: KB with Biochanin a diacetate 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0997 and Psyr_0998 are separated by 10 nucleotidesPsyr_0998 and Psyr_0999 overlap by 1 nucleotidesPsyr_0999 and Psyr_1000 are separated by 293 nucleotides Psyr_0997: Psyr_0997 - Endoribonuclease L-PSP, at 1,131,257 to 1,131,640 _0997 Psyr_0998: Psyr_0998 - Isochorismatase hydrolase, at 1,131,651 to 1,132,388 _0998 Psyr_0999: Psyr_0999 - luciferase, at 1,132,388 to 1,133,470 _0999 Psyr_1000: Psyr_1000 - transcriptional regulator, TetR family, at 1,133,764 to 1,134,492 _1000 Position (kb) 1132 1133 1134Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1131.512 kb on + strand, within Psyr_0997at 1131.512 kb on + strand, within Psyr_0997at 1131.513 kb on - strand, within Psyr_0997at 1131.555 kb on + strand, within Psyr_0997at 1131.555 kb on + strand, within Psyr_0997at 1131.555 kb on + strand, within Psyr_0997at 1131.555 kb on + strand, within Psyr_0997at 1131.555 kb on + strand, within Psyr_0997at 1131.555 kb on + strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.556 kb on - strand, within Psyr_0997at 1131.802 kb on + strand, within Psyr_0998at 1131.802 kb on + strand, within Psyr_0998at 1131.802 kb on + strand, within Psyr_0998at 1132.105 kb on + strand, within Psyr_0998at 1132.105 kb on + strand, within Psyr_0998at 1132.106 kb on - strand, within Psyr_0998at 1132.636 kb on + strand, within Psyr_0999at 1132.863 kb on + strand, within Psyr_0999at 1132.863 kb on + strand, within Psyr_0999at 1132.864 kb on - strand, within Psyr_0999at 1132.864 kb on - strand, within Psyr_0999at 1133.043 kb on + strand, within Psyr_0999at 1133.043 kb on + strand, within Psyr_0999at 1133.044 kb on - strand, within Psyr_0999at 1133.044 kb on - strand, within Psyr_0999at 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.403 kb on + strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.404 kb on - strandat 1133.549 kb on + strandat 1133.936 kb on + strand, within Psyr_1000at 1133.936 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.016 kb on + strand, within Psyr_1000at 1134.017 kb on - strand, within Psyr_1000at 1134.017 kb on - strand, within Psyr_1000at 1134.017 kb on - strand, within Psyr_1000at 1134.019 kb on + strand, within Psyr_1000at 1134.019 kb on + strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.020 kb on - strand, within Psyr_1000at 1134.044 kb on - strand, within Psyr_1000at 1134.075 kb on - strand, within Psyr_1000at 1134.136 kb on + strand, within Psyr_1000at 1134.137 kb on - strand, within Psyr_1000at 1134.137 kb on - strand, within Psyr_1000at 1134.137 kb on - strand, within Psyr_1000

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Biochanin a diacetate 10 mM
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1,131,512 + Psyr_0997 0.66 -0.3
1,131,512 + Psyr_0997 0.66 -0.4
1,131,513 - Psyr_0997 0.67 +4.6
1,131,555 + Psyr_0997 0.78 +1.2
1,131,555 + Psyr_0997 0.78 +0.2
1,131,555 + Psyr_0997 0.78 -1.9
1,131,555 + Psyr_0997 0.78 +1.6
1,131,555 + Psyr_0997 0.78 -0.3
1,131,555 + Psyr_0997 0.78 +0.2
1,131,556 - Psyr_0997 0.78 +0.8
1,131,556 - Psyr_0997 0.78 +0.9
1,131,556 - Psyr_0997 0.78 -0.6
1,131,556 - Psyr_0997 0.78 -1.2
1,131,556 - Psyr_0997 0.78 +0.9
1,131,556 - Psyr_0997 0.78 +0.8
1,131,556 - Psyr_0997 0.78 +1.9
1,131,556 - Psyr_0997 0.78 -0.4
1,131,556 - Psyr_0997 0.78 -2.1
1,131,802 + Psyr_0998 0.20 -2.0
1,131,802 + Psyr_0998 0.20 -0.0
1,131,802 + Psyr_0998 0.20 +0.7
1,132,105 + Psyr_0998 0.62 -0.1
1,132,105 + Psyr_0998 0.62 -0.2
1,132,106 - Psyr_0998 0.62 -0.9
1,132,636 + Psyr_0999 0.23 -0.8
1,132,863 + Psyr_0999 0.44 -1.1
1,132,863 + Psyr_0999 0.44 -0.5
1,132,864 - Psyr_0999 0.44 -3.2
1,132,864 - Psyr_0999 0.44 -0.6
1,133,043 + Psyr_0999 0.60 +0.4
1,133,043 + Psyr_0999 0.60 +1.0
1,133,044 - Psyr_0999 0.61 -1.1
1,133,044 - Psyr_0999 0.61 -0.6
1,133,403 + +1.4
1,133,403 + -1.0
1,133,403 + -0.6
1,133,403 + +1.1
1,133,403 + +0.3
1,133,404 - -0.2
1,133,404 - +0.3
1,133,404 - -0.7
1,133,404 - -0.0
1,133,404 - -0.0
1,133,404 - -0.9
1,133,404 - -0.0
1,133,549 + -0.3
1,133,936 + Psyr_1000 0.24 +0.8
1,133,936 + Psyr_1000 0.24 -0.5
1,134,016 + Psyr_1000 0.35 +0.6
1,134,016 + Psyr_1000 0.35 +0.1
1,134,016 + Psyr_1000 0.35 -0.8
1,134,016 + Psyr_1000 0.35 -0.8
1,134,016 + Psyr_1000 0.35 -0.7
1,134,017 - Psyr_1000 0.35 -1.1
1,134,017 - Psyr_1000 0.35 +0.2
1,134,017 - Psyr_1000 0.35 -0.8
1,134,019 + Psyr_1000 0.35 +1.2
1,134,019 + Psyr_1000 0.35 -0.1
1,134,020 - Psyr_1000 0.35 -2.3
1,134,020 - Psyr_1000 0.35 -0.3
1,134,020 - Psyr_1000 0.35 +0.4
1,134,020 - Psyr_1000 0.35 +0.8
1,134,044 - Psyr_1000 0.38 -0.1
1,134,075 - Psyr_1000 0.43 -0.8
1,134,136 + Psyr_1000 0.51 -0.3
1,134,137 - Psyr_1000 0.51 +0.8
1,134,137 - Psyr_1000 0.51 -0.8
1,134,137 - Psyr_1000 0.51 +0.0

Or see this region's nucleotide sequence