Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4385 and Psyr_4386 are separated by 330 nucleotides Psyr_4386 and Psyr_4387 are separated by 95 nucleotides
Psyr_4385: Psyr_4385 - Alpha-2-macroglobulin, N-terminal:Alpha-2-macroglobulin, N-terminal, at 5,242,625 to 5,247,583
_4385
Psyr_4386: Psyr_4386 - conserved hypothetical protein, at 5,247,914 to 5,248,207
_4386
Psyr_4387: Psyr_4387 - Glycosyl transferase, family 51:Penicillin-binding protein, transpeptidase:Penicillin-binding, C-terminal, at 5,248,303 to 5,250,651
_4387
Position (kb) 5247
5248
5249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5247.411 kb on - strand at 5247.411 kb on - strand at 5247.458 kb on + strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.521 kb on + strand at 5247.522 kb on - strand at 5247.522 kb on - strand at 5247.612 kb on + strand at 5247.658 kb on - strand at 5247.721 kb on - strand at 5247.760 kb on - strand at 5247.760 kb on - strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.288 kb on + strand at 5248.288 kb on + strand at 5248.289 kb on - strand at 5248.458 kb on + strand at 5248.532 kb on - strand at 5248.532 kb on - strand at 5248.532 kb on - strand at 5248.534 kb on + strand at 5248.534 kb on + strand at 5248.555 kb on + strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.644 kb on - strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.787 kb on - strand, within Psyr_4387 at 5248.846 kb on + strand, within Psyr_4387 at 5248.846 kb on + strand, within Psyr_4387
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 5,247,411 - +0.7 5,247,411 - +0.9 5,247,458 + -0.2 5,247,459 - -1.5 5,247,459 - +0.2 5,247,459 - +0.5 5,247,521 + -0.8 5,247,522 - -0.5 5,247,522 - +2.6 5,247,612 + +1.7 5,247,658 - +0.6 5,247,721 - -0.7 5,247,760 - -1.6 5,247,760 - -0.0 5,248,199 + -1.1 5,248,199 + -0.4 5,248,199 + +0.4 5,248,199 + -1.8 5,248,199 + -0.8 5,248,199 + -0.9 5,248,200 - +0.1 5,248,200 - -0.0 5,248,200 - -1.8 5,248,207 + -1.9 5,248,207 + -1.3 5,248,207 + +0.2 5,248,207 + +0.2 5,248,207 + -0.8 5,248,207 + +0.0 5,248,207 + -1.0 5,248,207 + -0.2 5,248,207 + +1.1 5,248,207 + -0.4 5,248,207 + -0.1 5,248,207 + +1.7 5,248,207 + -1.2 5,248,207 + -1.3 5,248,207 + -2.3 5,248,207 + +1.6 5,248,207 + +0.0 5,248,207 + +1.2 5,248,208 - -0.8 5,248,208 - +0.2 5,248,208 - -1.5 5,248,208 - +0.1 5,248,208 - -0.2 5,248,208 - -0.6 5,248,208 - +1.2 5,248,208 - -0.8 5,248,208 - -0.2 5,248,208 - -1.2 5,248,208 - +0.7 5,248,208 - -0.8 5,248,208 - -0.1 5,248,208 - -1.3 5,248,208 - -1.1 5,248,208 - +1.2 5,248,211 + -0.4 5,248,211 + -0.9 5,248,211 + +1.2 5,248,211 + -0.0 5,248,211 + +0.5 5,248,211 + +3.2 5,248,211 + -0.9 5,248,211 + +1.0 5,248,211 + -0.1 5,248,211 + +1.8 5,248,211 + +0.3 5,248,211 + +0.4 5,248,211 + -3.3 5,248,211 + +0.2 5,248,211 + +2.8 5,248,211 + -0.2 5,248,211 + -2.3 5,248,211 + -0.3 5,248,211 + +2.1 5,248,211 + -1.3 5,248,211 + +1.2 5,248,212 - +0.7 5,248,212 - +2.2 5,248,212 - +0.7 5,248,212 - +0.2 5,248,212 - -1.2 5,248,212 - +0.4 5,248,212 - +0.2 5,248,212 - +1.6 5,248,212 - +0.9 5,248,212 - +1.5 5,248,212 - +0.3 5,248,212 - +1.3 5,248,212 - -0.5 5,248,212 - -0.0 5,248,212 - +0.4 5,248,212 - +2.8 5,248,212 - -0.3 5,248,212 - +0.3 5,248,212 - -0.2 5,248,212 - -2.0 5,248,212 - +0.4 5,248,212 - +4.7 5,248,212 - -0.3 5,248,212 - -0.7 5,248,212 - -0.3 5,248,212 - -1.7 5,248,212 - -0.6 5,248,212 - -0.7 5,248,288 + -0.1 5,248,288 + -0.9 5,248,289 - +2.2 5,248,458 + -0.6 5,248,532 - +0.4 5,248,532 - +1.6 5,248,532 - -0.9 5,248,534 + +0.2 5,248,534 + -2.3 5,248,555 + Psyr_4387 0.11 +2.2 5,248,556 - Psyr_4387 0.11 -0.2 5,248,556 - Psyr_4387 0.11 +0.7 5,248,573 + Psyr_4387 0.11 -0.6 5,248,573 + Psyr_4387 0.11 +2.8 5,248,573 + Psyr_4387 0.11 -0.2 5,248,574 - Psyr_4387 0.12 +0.5 5,248,574 - Psyr_4387 0.12 -0.4 5,248,574 - Psyr_4387 0.12 -0.5 5,248,644 - Psyr_4387 0.15 +0.5 5,248,704 + Psyr_4387 0.17 -0.3 5,248,704 + Psyr_4387 0.17 -0.8 5,248,704 + Psyr_4387 0.17 -0.1 5,248,704 + Psyr_4387 0.17 +1.2 5,248,704 + Psyr_4387 0.17 -2.0 5,248,704 + Psyr_4387 0.17 -0.7 5,248,704 + Psyr_4387 0.17 +1.1 5,248,705 - Psyr_4387 0.17 -2.5 5,248,705 - Psyr_4387 0.17 -1.3 5,248,705 - Psyr_4387 0.17 -1.4 5,248,705 - Psyr_4387 0.17 +1.6 5,248,705 - Psyr_4387 0.17 -1.8 5,248,705 - Psyr_4387 0.17 +1.8 5,248,787 - Psyr_4387 0.21 +0.4 5,248,846 + Psyr_4387 0.23 -0.9 5,248,846 + Psyr_4387 0.23 -2.1
Or see this region's nucleotide sequence