Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4133 and Psyr_4134 are separated by 123 nucleotides Psyr_4134 and Psyr_4135 are separated by 153 nucleotides Psyr_4135 and Psyr_4136 are separated by 22 nucleotides Psyr_4136 and Psyr_4137 are separated by 115 nucleotides Psyr_4137 and Psyr_4138 overlap by 4 nucleotides
Psyr_4133: Psyr_4133 - histidinol dehydrogenase, at 4,923,796 to 4,925,142
_4133
Psyr_4134: Psyr_4134 - ATP phosphoribosyltransferase (homohexameric), at 4,925,266 to 4,925,901
_4134
Psyr_4135: Psyr_4135 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 4,926,055 to 4,927,320
_4135
Psyr_4136: Psyr_4136 - BolA-like protein, at 4,927,343 to 4,927,582
_4136
Psyr_4137: Psyr_4137 - Sulfate transporter/antisigma-factor antagonist STAS, at 4,927,698 to 4,928,003
_4137
Psyr_4138: Psyr_4138 - Toluene tolerance, at 4,928,000 to 4,928,650
_4138
Position (kb) 4926
4927
4928 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4925.156 kb on + strand at 4925.156 kb on + strand at 4925.157 kb on - strand at 4925.185 kb on + strand at 4925.185 kb on + strand at 4925.194 kb on - strand at 4925.492 kb on + strand, within Psyr_4134 at 4925.492 kb on + strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.532 kb on - strand, within Psyr_4134 at 4925.532 kb on - strand, within Psyr_4134 at 4925.534 kb on + strand, within Psyr_4134 at 4925.534 kb on + strand, within Psyr_4134 at 4925.535 kb on - strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.655 kb on - strand, within Psyr_4134 at 4925.672 kb on + strand, within Psyr_4134 at 4925.672 kb on + strand, within Psyr_4134 at 4925.672 kb on + strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.910 kb on + strand at 4925.910 kb on + strand at 4925.910 kb on + strand at 4925.911 kb on - strand at 4925.911 kb on - strand at 4927.435 kb on - strand, within Psyr_4136 at 4927.435 kb on - strand, within Psyr_4136 at 4927.572 kb on - strand at 4927.627 kb on - strand at 4927.645 kb on - strand at 4927.741 kb on - strand, within Psyr_4137 at 4927.741 kb on - strand, within Psyr_4137 at 4928.033 kb on - strand at 4928.202 kb on + strand, within Psyr_4138 at 4928.202 kb on + strand, within Psyr_4138 at 4928.203 kb on - strand, within Psyr_4138 at 4928.203 kb on - strand, within Psyr_4138 at 4928.214 kb on + strand, within Psyr_4138
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 4,925,156 + -0.5 4,925,156 + +1.1 4,925,157 - +0.8 4,925,185 + -1.4 4,925,185 + -1.7 4,925,194 - -0.8 4,925,492 + Psyr_4134 0.36 +2.7 4,925,492 + Psyr_4134 0.36 -1.2 4,925,493 - Psyr_4134 0.36 -1.3 4,925,493 - Psyr_4134 0.36 +0.1 4,925,493 - Psyr_4134 0.36 +2.7 4,925,493 - Psyr_4134 0.36 -2.0 4,925,532 - Psyr_4134 0.42 -0.6 4,925,532 - Psyr_4134 0.42 -0.9 4,925,534 + Psyr_4134 0.42 -1.9 4,925,534 + Psyr_4134 0.42 -0.4 4,925,535 - Psyr_4134 0.42 +0.0 4,925,654 + Psyr_4134 0.61 -0.0 4,925,654 + Psyr_4134 0.61 -0.1 4,925,654 + Psyr_4134 0.61 -2.5 4,925,654 + Psyr_4134 0.61 -3.7 4,925,655 - Psyr_4134 0.61 -0.9 4,925,672 + Psyr_4134 0.64 -1.0 4,925,672 + Psyr_4134 0.64 -1.4 4,925,672 + Psyr_4134 0.64 -1.5 4,925,673 - Psyr_4134 0.64 -0.6 4,925,673 - Psyr_4134 0.64 -3.0 4,925,673 - Psyr_4134 0.64 +0.9 4,925,673 - Psyr_4134 0.64 -2.6 4,925,729 + Psyr_4134 0.73 -0.4 4,925,729 + Psyr_4134 0.73 +0.3 4,925,729 + Psyr_4134 0.73 +1.1 4,925,729 + Psyr_4134 0.73 +1.6 4,925,729 + Psyr_4134 0.73 +1.1 4,925,729 + Psyr_4134 0.73 -0.5 4,925,729 + Psyr_4134 0.73 +3.1 4,925,730 - Psyr_4134 0.73 -0.1 4,925,730 - Psyr_4134 0.73 +2.1 4,925,730 - Psyr_4134 0.73 +1.9 4,925,730 - Psyr_4134 0.73 -0.4 4,925,730 - Psyr_4134 0.73 +1.1 4,925,730 - Psyr_4134 0.73 -1.4 4,925,730 - Psyr_4134 0.73 -0.1 4,925,730 - Psyr_4134 0.73 +0.6 4,925,910 + -1.1 4,925,910 + -0.6 4,925,910 + -0.9 4,925,911 - -0.9 4,925,911 - +2.1 4,927,435 - Psyr_4136 0.38 -1.3 4,927,435 - Psyr_4136 0.38 +1.3 4,927,572 - +1.1 4,927,627 - -1.4 4,927,645 - +1.1 4,927,741 - Psyr_4137 0.14 +0.8 4,927,741 - Psyr_4137 0.14 -1.0 4,928,033 - -1.1 4,928,202 + Psyr_4138 0.31 -1.2 4,928,202 + Psyr_4138 0.31 -0.6 4,928,203 - Psyr_4138 0.31 -2.6 4,928,203 - Psyr_4138 0.31 -0.9 4,928,214 + Psyr_4138 0.33 +2.7
Or see this region's nucleotide sequence