Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3843 and Psyr_3844 are separated by 150 nucleotides Psyr_3844 and Psyr_3845 are separated by 325 nucleotides Psyr_3845 and Psyr_3846 are separated by 227 nucleotides
Psyr_3843: Psyr_3843 - diguanylate cyclase/phosphodiesterase, at 4,580,648 to 4,582,939
_3843
Psyr_3844: Psyr_3844 - conserved hypothetical protein, at 4,583,090 to 4,583,395
_3844
Psyr_3845: Psyr_3845 - L-leucine-binding protein / L-valine-binding protein / L-isoleucine-binding protein, at 4,583,721 to 4,584,845
_3845
Psyr_3846: Psyr_3846 - L-valine ABC transporter membrane protein / L-isoleucine ABC transporter membrane protein / L-leucine ABC transporter membrane protein, at 4,585,073 to 4,585,996
_3846
Position (kb) 4583
4584
4585 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4582.724 kb on + strand at 4582.724 kb on + strand at 4582.724 kb on + strand at 4582.725 kb on - strand at 4582.725 kb on - strand at 4582.728 kb on + strand at 4582.728 kb on + strand at 4582.728 kb on + strand at 4582.728 kb on + strand at 4582.729 kb on - strand at 4582.729 kb on - strand at 4582.729 kb on - strand at 4582.729 kb on - strand at 4582.729 kb on - strand at 4582.729 kb on - strand at 4582.736 kb on + strand at 4582.736 kb on + strand at 4582.736 kb on + strand at 4582.736 kb on + strand at 4582.736 kb on + strand at 4582.737 kb on - strand at 4582.737 kb on - strand at 4582.737 kb on - strand at 4582.737 kb on - strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.738 kb on + strand at 4582.739 kb on - strand at 4582.739 kb on - strand at 4582.739 kb on - strand at 4582.739 kb on - strand at 4582.739 kb on - strand at 4582.739 kb on - strand at 4582.839 kb on - strand at 4583.015 kb on - strand at 4583.040 kb on - strand at 4583.040 kb on - strand at 4583.133 kb on - strand, within Psyr_3844 at 4583.135 kb on + strand, within Psyr_3844 at 4583.370 kb on + strand at 4583.380 kb on + strand at 4583.380 kb on + strand at 4583.380 kb on + strand at 4583.471 kb on - strand at 4583.495 kb on + strand at 4583.615 kb on + strand at 4583.690 kb on + strand at 4583.690 kb on + strand at 4583.691 kb on - strand at 4583.692 kb on + strand at 4583.692 kb on + strand at 4583.693 kb on - strand at 4583.693 kb on - strand at 4583.700 kb on - strand at 4583.726 kb on + strand at 4583.727 kb on - strand at 4583.739 kb on - strand at 4583.917 kb on + strand, within Psyr_3845 at 4583.917 kb on + strand, within Psyr_3845 at 4583.917 kb on + strand, within Psyr_3845 at 4583.917 kb on + strand, within Psyr_3845 at 4583.918 kb on - strand, within Psyr_3845 at 4583.918 kb on - strand, within Psyr_3845 at 4583.918 kb on - strand, within Psyr_3845 at 4583.918 kb on - strand, within Psyr_3845 at 4583.946 kb on + strand, within Psyr_3845 at 4583.946 kb on + strand, within Psyr_3845 at 4583.946 kb on + strand, within Psyr_3845 at 4583.946 kb on + strand, within Psyr_3845 at 4584.015 kb on + strand, within Psyr_3845 at 4584.015 kb on + strand, within Psyr_3845 at 4584.015 kb on + strand, within Psyr_3845 at 4584.015 kb on + strand, within Psyr_3845 at 4584.015 kb on + strand, within Psyr_3845 at 4584.016 kb on - strand, within Psyr_3845 at 4584.192 kb on + strand, within Psyr_3845 at 4584.192 kb on + strand, within Psyr_3845 at 4584.192 kb on + strand, within Psyr_3845 at 4584.192 kb on + strand, within Psyr_3845 at 4584.192 kb on + strand, within Psyr_3845 at 4584.192 kb on + strand, within Psyr_3845 at 4584.193 kb on - strand, within Psyr_3845 at 4584.193 kb on - strand, within Psyr_3845 at 4584.193 kb on - strand, within Psyr_3845 at 4584.193 kb on - strand, within Psyr_3845 at 4584.193 kb on - strand, within Psyr_3845 at 4584.193 kb on - strand, within Psyr_3845 at 4584.193 kb on - strand, within Psyr_3845 at 4584.393 kb on + strand, within Psyr_3845 at 4584.393 kb on + strand, within Psyr_3845 at 4584.394 kb on - strand, within Psyr_3845 at 4584.643 kb on - strand, within Psyr_3845 at 4584.759 kb on + strand at 4584.760 kb on - strand at 4584.801 kb on + strand at 4584.801 kb on + strand at 4584.843 kb on + strand at 4584.843 kb on + strand at 4584.844 kb on - strand at 4584.844 kb on - strand at 4584.844 kb on - strand at 4584.939 kb on + strand at 4584.939 kb on + strand at 4584.940 kb on - strand at 4584.940 kb on - strand at 4584.940 kb on - strand at 4584.940 kb on - strand at 4584.964 kb on + strand at 4584.964 kb on + strand at 4584.976 kb on + strand at 4585.070 kb on + strand at 4585.071 kb on - strand at 4585.091 kb on + strand at 4585.091 kb on + strand at 4585.091 kb on + strand at 4585.091 kb on + strand at 4585.091 kb on + strand at 4585.091 kb on + strand at 4585.091 kb on + strand at 4585.092 kb on - strand at 4585.092 kb on - strand at 4585.092 kb on - strand at 4585.092 kb on - strand at 4585.092 kb on - strand at 4585.092 kb on - strand at 4585.092 kb on - strand at 4585.129 kb on + strand at 4585.129 kb on + strand at 4585.129 kb on + strand at 4585.129 kb on + strand at 4585.130 kb on - strand at 4585.130 kb on - strand at 4585.133 kb on + strand at 4585.133 kb on + strand at 4585.133 kb on + strand at 4585.133 kb on + strand at 4585.133 kb on + strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.134 kb on - strand at 4585.166 kb on + strand, within Psyr_3846 at 4585.167 kb on - strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.208 kb on + strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.209 kb on - strand, within Psyr_3846 at 4585.223 kb on + strand, within Psyr_3846 at 4585.223 kb on + strand, within Psyr_3846 at 4585.223 kb on + strand, within Psyr_3846 at 4585.223 kb on + strand, within Psyr_3846 at 4585.223 kb on + strand, within Psyr_3846 at 4585.224 kb on - strand, within Psyr_3846 at 4585.224 kb on - strand, within Psyr_3846 at 4585.224 kb on - strand, within Psyr_3846 at 4585.224 kb on - strand, within Psyr_3846 at 4585.224 kb on - strand, within Psyr_3846 at 4585.334 kb on + strand, within Psyr_3846 at 4585.533 kb on - strand, within Psyr_3846
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 4,582,724 + +0.3 4,582,724 + +0.0 4,582,724 + -0.9 4,582,725 - -0.9 4,582,725 - +0.5 4,582,728 + -0.9 4,582,728 + +0.6 4,582,728 + +3.1 4,582,728 + +0.6 4,582,729 - +0.3 4,582,729 - -1.2 4,582,729 - -0.9 4,582,729 - -1.2 4,582,729 - +0.9 4,582,729 - -0.6 4,582,736 + +0.8 4,582,736 + -1.0 4,582,736 + -2.6 4,582,736 + -0.3 4,582,736 + +0.1 4,582,737 - +0.1 4,582,737 - -0.1 4,582,737 - -0.6 4,582,737 - -0.8 4,582,738 + -0.0 4,582,738 + +0.5 4,582,738 + +3.7 4,582,738 + +1.0 4,582,738 + -1.3 4,582,738 + -0.0 4,582,738 + +0.5 4,582,738 + -0.3 4,582,738 + +0.7 4,582,738 + -1.6 4,582,738 + +0.3 4,582,738 + -2.2 4,582,739 - -1.6 4,582,739 - +0.4 4,582,739 - -0.4 4,582,739 - -0.7 4,582,739 - -1.6 4,582,739 - +0.0 4,582,839 - +0.3 4,583,015 - -1.4 4,583,040 - -0.9 4,583,040 - -1.5 4,583,133 - Psyr_3844 0.14 +2.8 4,583,135 + Psyr_3844 0.15 -0.7 4,583,370 + -0.8 4,583,380 + -0.4 4,583,380 + +1.2 4,583,380 + -0.6 4,583,471 - -0.3 4,583,495 + +1.3 4,583,615 + -2.0 4,583,690 + +4.3 4,583,690 + -1.4 4,583,691 - -0.4 4,583,692 + -0.7 4,583,692 + +0.0 4,583,693 - -1.2 4,583,693 - -1.0 4,583,700 - +0.1 4,583,726 + +0.1 4,583,727 - -0.6 4,583,739 - +0.6 4,583,917 + Psyr_3845 0.17 -0.0 4,583,917 + Psyr_3845 0.17 -0.5 4,583,917 + Psyr_3845 0.17 -2.0 4,583,917 + Psyr_3845 0.17 -1.2 4,583,918 - Psyr_3845 0.18 -0.5 4,583,918 - Psyr_3845 0.18 +0.4 4,583,918 - Psyr_3845 0.18 +0.6 4,583,918 - Psyr_3845 0.18 +0.5 4,583,946 + Psyr_3845 0.20 +0.5 4,583,946 + Psyr_3845 0.20 +0.9 4,583,946 + Psyr_3845 0.20 +1.4 4,583,946 + Psyr_3845 0.20 +0.6 4,584,015 + Psyr_3845 0.26 -0.1 4,584,015 + Psyr_3845 0.26 -0.0 4,584,015 + Psyr_3845 0.26 -0.1 4,584,015 + Psyr_3845 0.26 +1.4 4,584,015 + Psyr_3845 0.26 -2.2 4,584,016 - Psyr_3845 0.26 -0.1 4,584,192 + Psyr_3845 0.42 -0.2 4,584,192 + Psyr_3845 0.42 +0.8 4,584,192 + Psyr_3845 0.42 +0.3 4,584,192 + Psyr_3845 0.42 -0.8 4,584,192 + Psyr_3845 0.42 +0.0 4,584,192 + Psyr_3845 0.42 -1.2 4,584,193 - Psyr_3845 0.42 -0.4 4,584,193 - Psyr_3845 0.42 -0.4 4,584,193 - Psyr_3845 0.42 -0.3 4,584,193 - Psyr_3845 0.42 +0.8 4,584,193 - Psyr_3845 0.42 +1.0 4,584,193 - Psyr_3845 0.42 -0.0 4,584,193 - Psyr_3845 0.42 -1.2 4,584,393 + Psyr_3845 0.60 +0.2 4,584,393 + Psyr_3845 0.60 -1.8 4,584,394 - Psyr_3845 0.60 -0.8 4,584,643 - Psyr_3845 0.82 +2.5 4,584,759 + +0.8 4,584,760 - -0.3 4,584,801 + -1.0 4,584,801 + -2.0 4,584,843 + -0.4 4,584,843 + +0.1 4,584,844 - -0.4 4,584,844 - +0.6 4,584,844 - -0.6 4,584,939 + +0.3 4,584,939 + +0.0 4,584,940 - +0.8 4,584,940 - +2.2 4,584,940 - +0.2 4,584,940 - -0.5 4,584,964 + -0.4 4,584,964 + +3.1 4,584,976 + +0.2 4,585,070 + +0.3 4,585,071 - -0.4 4,585,091 + -0.3 4,585,091 + +0.4 4,585,091 + -0.2 4,585,091 + -2.0 4,585,091 + -0.9 4,585,091 + -0.6 4,585,091 + +0.5 4,585,092 - +0.5 4,585,092 - -0.1 4,585,092 - +0.8 4,585,092 - -0.9 4,585,092 - -0.2 4,585,092 - -1.8 4,585,092 - -0.5 4,585,129 + +0.2 4,585,129 + +2.4 4,585,129 + -0.6 4,585,129 + +0.5 4,585,130 - -1.3 4,585,130 - -1.1 4,585,133 + -0.0 4,585,133 + -0.3 4,585,133 + +0.8 4,585,133 + -1.5 4,585,133 + -1.2 4,585,134 - +1.3 4,585,134 - +0.7 4,585,134 - -0.1 4,585,134 - +0.4 4,585,134 - +3.5 4,585,134 - -0.1 4,585,134 - -0.6 4,585,134 - -0.3 4,585,134 - -0.2 4,585,134 - +0.5 4,585,134 - -0.1 4,585,134 - +0.1 4,585,134 - -1.3 4,585,134 - -0.8 4,585,134 - +0.2 4,585,166 + Psyr_3846 0.10 -0.7 4,585,167 - Psyr_3846 0.10 +0.4 4,585,208 + Psyr_3846 0.15 +0.1 4,585,208 + Psyr_3846 0.15 +0.3 4,585,208 + Psyr_3846 0.15 +2.3 4,585,208 + Psyr_3846 0.15 -0.2 4,585,208 + Psyr_3846 0.15 -0.5 4,585,208 + Psyr_3846 0.15 -0.0 4,585,208 + Psyr_3846 0.15 -0.1 4,585,208 + Psyr_3846 0.15 -1.5 4,585,208 + Psyr_3846 0.15 +1.5 4,585,208 + Psyr_3846 0.15 +1.4 4,585,208 + Psyr_3846 0.15 -0.7 4,585,209 - Psyr_3846 0.15 +0.2 4,585,209 - Psyr_3846 0.15 +0.4 4,585,209 - Psyr_3846 0.15 -0.5 4,585,209 - Psyr_3846 0.15 +0.8 4,585,209 - Psyr_3846 0.15 +0.6 4,585,209 - Psyr_3846 0.15 +1.8 4,585,209 - Psyr_3846 0.15 -1.3 4,585,209 - Psyr_3846 0.15 -1.7 4,585,209 - Psyr_3846 0.15 -2.0 4,585,209 - Psyr_3846 0.15 -0.5 4,585,209 - Psyr_3846 0.15 -0.7 4,585,209 - Psyr_3846 0.15 +0.6 4,585,209 - Psyr_3846 0.15 -0.2 4,585,209 - Psyr_3846 0.15 -0.6 4,585,209 - Psyr_3846 0.15 +2.1 4,585,209 - Psyr_3846 0.15 -0.5 4,585,209 - Psyr_3846 0.15 -0.6 4,585,223 + Psyr_3846 0.16 +0.4 4,585,223 + Psyr_3846 0.16 +0.2 4,585,223 + Psyr_3846 0.16 -0.7 4,585,223 + Psyr_3846 0.16 +0.5 4,585,223 + Psyr_3846 0.16 +1.1 4,585,224 - Psyr_3846 0.16 -1.3 4,585,224 - Psyr_3846 0.16 +0.6 4,585,224 - Psyr_3846 0.16 -0.1 4,585,224 - Psyr_3846 0.16 -0.4 4,585,224 - Psyr_3846 0.16 -1.0 4,585,334 + Psyr_3846 0.28 -2.4 4,585,533 - Psyr_3846 0.50 -0.8
Or see this region's nucleotide sequence