Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2394 and Psyr_2395 are separated by 101 nucleotides Psyr_2395 and Psyr_2396 overlap by 4 nucleotides Psyr_2396 and Psyr_2397 are separated by 97 nucleotides
Psyr_2394: Psyr_2394 - succinate semialdehyde dehydrogenase, at 2,775,689 to 2,777,080
_2394
Psyr_2395: Psyr_2395 - transcriptional regulator, LysR family, at 2,777,182 to 2,778,057
_2395
Psyr_2396: Psyr_2396 - Tartrate dehydrogenase, at 2,778,054 to 2,779,175
_2396
Psyr_2397: Psyr_2397 - transcriptional regulator, LysR family, at 2,779,273 to 2,780,187
_2397
Position (kb)
2778
2779
2780 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2777.074 kb on + strand at 2777.075 kb on - strand at 2777.075 kb on - strand at 2777.116 kb on + strand at 2777.116 kb on + strand at 2777.118 kb on + strand at 2777.118 kb on + strand at 2777.118 kb on + strand at 2777.118 kb on + strand at 2777.119 kb on - strand at 2777.119 kb on - strand at 2777.119 kb on - strand at 2777.380 kb on + strand, within Psyr_2395 at 2777.512 kb on + strand, within Psyr_2395 at 2777.513 kb on - strand, within Psyr_2395 at 2777.592 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.733 kb on + strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.734 kb on - strand, within Psyr_2395 at 2777.738 kb on - strand, within Psyr_2395 at 2777.738 kb on - strand, within Psyr_2395 at 2777.738 kb on - strand, within Psyr_2395 at 2777.834 kb on - strand, within Psyr_2395 at 2777.834 kb on - strand, within Psyr_2395 at 2778.997 kb on + strand, within Psyr_2396 at 2778.997 kb on + strand, within Psyr_2396 at 2779.021 kb on + strand, within Psyr_2396 at 2779.022 kb on - strand, within Psyr_2396 at 2779.128 kb on - strand at 2779.216 kb on + strand at 2779.231 kb on + strand at 2779.238 kb on - strand at 2779.278 kb on + strand at 2779.375 kb on + strand, within Psyr_2397 at 2779.375 kb on + strand, within Psyr_2397 at 2779.389 kb on + strand, within Psyr_2397 at 2779.390 kb on - strand, within Psyr_2397 at 2779.390 kb on - strand, within Psyr_2397 at 2779.390 kb on - strand, within Psyr_2397 at 2779.477 kb on + strand, within Psyr_2397 at 2779.478 kb on - strand, within Psyr_2397 at 2780.152 kb on + strand at 2780.152 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 2,777,074 + +1.3 2,777,075 - +0.3 2,777,075 - -0.9 2,777,116 + -0.2 2,777,116 + -0.3 2,777,118 + +1.6 2,777,118 + -1.2 2,777,118 + -0.5 2,777,118 + +1.6 2,777,119 - +1.3 2,777,119 - +0.3 2,777,119 - -0.3 2,777,380 + Psyr_2395 0.23 +1.9 2,777,512 + Psyr_2395 0.38 -0.3 2,777,513 - Psyr_2395 0.38 +1.8 2,777,592 + Psyr_2395 0.47 -0.4 2,777,733 + Psyr_2395 0.63 +1.5 2,777,733 + Psyr_2395 0.63 +0.2 2,777,733 + Psyr_2395 0.63 -1.9 2,777,733 + Psyr_2395 0.63 -0.9 2,777,733 + Psyr_2395 0.63 -0.6 2,777,734 - Psyr_2395 0.63 -0.5 2,777,734 - Psyr_2395 0.63 +1.5 2,777,734 - Psyr_2395 0.63 -0.5 2,777,734 - Psyr_2395 0.63 -1.0 2,777,734 - Psyr_2395 0.63 +1.3 2,777,734 - Psyr_2395 0.63 +0.1 2,777,738 - Psyr_2395 0.63 -0.7 2,777,738 - Psyr_2395 0.63 +0.7 2,777,738 - Psyr_2395 0.63 +0.0 2,777,834 - Psyr_2395 0.74 -0.7 2,777,834 - Psyr_2395 0.74 -1.0 2,778,997 + Psyr_2396 0.84 -0.6 2,778,997 + Psyr_2396 0.84 +1.3 2,779,021 + Psyr_2396 0.86 -0.9 2,779,022 - Psyr_2396 0.86 -3.4 2,779,128 - -0.3 2,779,216 + +0.9 2,779,231 + -0.3 2,779,238 - -1.8 2,779,278 + +0.6 2,779,375 + Psyr_2397 0.11 +1.5 2,779,375 + Psyr_2397 0.11 +2.3 2,779,389 + Psyr_2397 0.13 +0.3 2,779,390 - Psyr_2397 0.13 -1.0 2,779,390 - Psyr_2397 0.13 -0.2 2,779,390 - Psyr_2397 0.13 +0.3 2,779,477 + Psyr_2397 0.22 +1.3 2,779,478 - Psyr_2397 0.22 -0.6 2,780,152 + +2.0 2,780,152 + -1.0
Or see this region's nucleotide sequence