Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1403 and Psyr_1404 are separated by 845 nucleotides Psyr_1404 and Psyr_1405 are separated by 341 nucleotides Psyr_1405 and Psyr_1406 are separated by 292 nucleotides
Psyr_1403: Psyr_1403 - cold-shock DNA-binding protein family, at 1,589,661 to 1,589,882
_1403
Psyr_1404: Psyr_1404 - Ferritin and Dps, at 1,590,728 to 1,591,201
_1404
Psyr_1405: Psyr_1405 - hypothetical protein, at 1,591,543 to 1,591,887
_1405
Psyr_1406: Psyr_1406 - aspartate--tRNA(Asn) ligase, at 1,592,180 to 1,593,955
_1406
Position (kb) 1590
1591
1592 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 1589.914 kb on + strand at 1589.914 kb on + strand at 1589.915 kb on - strand at 1589.915 kb on - strand at 1589.915 kb on - strand at 1589.915 kb on - strand at 1590.028 kb on - strand at 1590.036 kb on + strand at 1590.037 kb on - strand at 1590.083 kb on + strand at 1590.084 kb on - strand at 1590.084 kb on - strand at 1590.145 kb on - strand at 1590.182 kb on - strand at 1590.200 kb on + strand at 1590.209 kb on + strand at 1590.210 kb on - strand at 1590.213 kb on + strand at 1590.306 kb on + strand at 1590.306 kb on + strand at 1590.306 kb on + strand at 1590.306 kb on + strand at 1590.371 kb on + strand at 1590.371 kb on + strand at 1590.371 kb on + strand at 1590.372 kb on - strand at 1590.372 kb on - strand at 1590.372 kb on - strand at 1590.372 kb on - strand at 1590.415 kb on - strand at 1590.437 kb on + strand at 1590.500 kb on + strand at 1590.501 kb on - strand at 1590.503 kb on + strand at 1590.503 kb on + strand at 1590.503 kb on + strand at 1590.504 kb on - strand at 1590.504 kb on - strand at 1590.504 kb on - strand at 1590.504 kb on - strand at 1590.550 kb on + strand at 1590.551 kb on - strand at 1590.641 kb on + strand at 1590.641 kb on + strand at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.806 kb on + strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.807 kb on - strand, within Psyr_1404 at 1590.893 kb on + strand, within Psyr_1404 at 1590.893 kb on + strand, within Psyr_1404 at 1590.894 kb on - strand, within Psyr_1404 at 1590.894 kb on - strand, within Psyr_1404 at 1590.894 kb on - strand, within Psyr_1404 at 1590.894 kb on - strand, within Psyr_1404 at 1590.894 kb on - strand, within Psyr_1404 at 1590.894 kb on - strand, within Psyr_1404 at 1590.969 kb on - strand, within Psyr_1404 at 1590.978 kb on - strand, within Psyr_1404 at 1591.030 kb on + strand, within Psyr_1404 at 1591.031 kb on - strand, within Psyr_1404 at 1591.031 kb on - strand, within Psyr_1404 at 1591.031 kb on - strand, within Psyr_1404 at 1591.072 kb on + strand, within Psyr_1404 at 1591.081 kb on + strand, within Psyr_1404 at 1591.081 kb on + strand, within Psyr_1404 at 1591.081 kb on + strand, within Psyr_1404 at 1591.082 kb on - strand, within Psyr_1404 at 1591.085 kb on - strand, within Psyr_1404 at 1591.199 kb on + strand at 1591.200 kb on - strand at 1591.200 kb on - strand at 1591.266 kb on - strand at 1591.281 kb on + strand at 1591.282 kb on - strand at 1591.383 kb on + strand at 1591.468 kb on + strand at 1591.470 kb on + strand at 1591.485 kb on - strand at 1591.572 kb on + strand at 1591.572 kb on + strand at 1591.573 kb on - strand at 1591.599 kb on - strand, within Psyr_1405 at 1591.630 kb on - strand, within Psyr_1405 at 1591.705 kb on + strand, within Psyr_1405 at 1591.706 kb on - strand, within Psyr_1405 at 1591.761 kb on - strand, within Psyr_1405 at 1591.769 kb on - strand, within Psyr_1405 at 1591.769 kb on - strand, within Psyr_1405 at 1591.885 kb on + strand at 1591.885 kb on + strand at 1591.890 kb on + strand at 1591.890 kb on + strand at 1591.890 kb on + strand at 1591.890 kb on + strand at 1591.891 kb on - strand at 1591.891 kb on - strand at 1591.998 kb on + strand at 1592.029 kb on + strand at 1592.029 kb on + strand at 1592.029 kb on + strand at 1592.029 kb on + strand at 1592.029 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.035 kb on + strand at 1592.037 kb on + strand at 1592.037 kb on + strand at 1592.037 kb on + strand at 1592.037 kb on + strand at 1592.037 kb on + strand at 1592.037 kb on + strand at 1592.039 kb on + strand at 1592.039 kb on + strand at 1592.039 kb on + strand at 1592.039 kb on + strand at 1592.039 kb on + strand at 1592.039 kb on + strand at 1592.039 kb on + strand at 1592.112 kb on + strand at 1592.158 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 1,589,914 + -2.6 1,589,914 + -1.7 1,589,915 - +1.7 1,589,915 - -0.7 1,589,915 - +1.5 1,589,915 - +2.3 1,590,028 - +0.9 1,590,036 + -3.9 1,590,037 - -2.6 1,590,083 + -0.1 1,590,084 - +0.3 1,590,084 - -1.9 1,590,145 - -2.2 1,590,182 - -2.1 1,590,200 + -2.4 1,590,209 + -2.2 1,590,210 - -1.9 1,590,213 + -1.4 1,590,306 + +0.7 1,590,306 + -0.4 1,590,306 + -1.6 1,590,306 + -0.9 1,590,371 + -2.4 1,590,371 + -1.6 1,590,371 + -1.1 1,590,372 - -1.0 1,590,372 - +0.3 1,590,372 - +0.3 1,590,372 - -0.5 1,590,415 - +0.0 1,590,437 + -1.4 1,590,500 + +2.4 1,590,501 - -0.7 1,590,503 + +0.6 1,590,503 + +0.1 1,590,503 + +1.9 1,590,504 - -0.1 1,590,504 - -2.1 1,590,504 - -0.6 1,590,504 - -0.4 1,590,550 + +0.9 1,590,551 - +0.4 1,590,641 + -0.9 1,590,641 + +1.3 1,590,806 + Psyr_1404 0.16 -1.3 1,590,806 + Psyr_1404 0.16 +1.7 1,590,806 + Psyr_1404 0.16 +2.8 1,590,806 + Psyr_1404 0.16 -2.1 1,590,806 + Psyr_1404 0.16 +1.1 1,590,806 + Psyr_1404 0.16 -0.1 1,590,806 + Psyr_1404 0.16 -0.7 1,590,806 + Psyr_1404 0.16 -1.8 1,590,806 + Psyr_1404 0.16 -0.1 1,590,806 + Psyr_1404 0.16 -1.5 1,590,806 + Psyr_1404 0.16 -0.1 1,590,806 + Psyr_1404 0.16 -3.2 1,590,806 + Psyr_1404 0.16 -1.0 1,590,806 + Psyr_1404 0.16 -1.6 1,590,807 - Psyr_1404 0.17 -0.2 1,590,807 - Psyr_1404 0.17 +0.0 1,590,807 - Psyr_1404 0.17 -0.9 1,590,807 - Psyr_1404 0.17 +0.3 1,590,807 - Psyr_1404 0.17 -1.2 1,590,807 - Psyr_1404 0.17 +1.0 1,590,807 - Psyr_1404 0.17 -0.4 1,590,807 - Psyr_1404 0.17 -2.5 1,590,807 - Psyr_1404 0.17 -0.2 1,590,807 - Psyr_1404 0.17 -2.0 1,590,893 + Psyr_1404 0.35 +0.3 1,590,893 + Psyr_1404 0.35 -0.7 1,590,894 - Psyr_1404 0.35 +0.0 1,590,894 - Psyr_1404 0.35 -0.7 1,590,894 - Psyr_1404 0.35 +2.3 1,590,894 - Psyr_1404 0.35 -0.7 1,590,894 - Psyr_1404 0.35 -0.2 1,590,894 - Psyr_1404 0.35 +1.8 1,590,969 - Psyr_1404 0.51 +3.3 1,590,978 - Psyr_1404 0.53 +0.1 1,591,030 + Psyr_1404 0.64 +0.4 1,591,031 - Psyr_1404 0.64 -0.6 1,591,031 - Psyr_1404 0.64 -0.4 1,591,031 - Psyr_1404 0.64 +0.4 1,591,072 + Psyr_1404 0.73 +1.8 1,591,081 + Psyr_1404 0.74 -0.6 1,591,081 + Psyr_1404 0.74 +0.1 1,591,081 + Psyr_1404 0.74 +0.3 1,591,082 - Psyr_1404 0.75 -1.9 1,591,085 - Psyr_1404 0.75 -1.3 1,591,199 + +1.0 1,591,200 - -0.1 1,591,200 - -2.6 1,591,266 - +1.5 1,591,281 + -1.7 1,591,282 - +0.0 1,591,383 + +0.9 1,591,468 + -1.3 1,591,470 + +0.7 1,591,485 - +0.4 1,591,572 + +0.8 1,591,572 + +1.3 1,591,573 - -1.4 1,591,599 - Psyr_1405 0.16 +1.3 1,591,630 - Psyr_1405 0.25 -1.3 1,591,705 + Psyr_1405 0.47 +0.3 1,591,706 - Psyr_1405 0.47 -1.5 1,591,761 - Psyr_1405 0.63 -1.3 1,591,769 - Psyr_1405 0.66 -0.3 1,591,769 - Psyr_1405 0.66 +2.1 1,591,885 + +0.4 1,591,885 + -0.4 1,591,890 + -0.1 1,591,890 + -1.1 1,591,890 + +0.1 1,591,890 + -0.5 1,591,891 - +0.1 1,591,891 - +0.3 1,591,998 + +0.7 1,592,029 + -0.5 1,592,029 + +1.8 1,592,029 + +3.0 1,592,029 + +0.6 1,592,029 + -1.2 1,592,035 + +0.8 1,592,035 + +0.3 1,592,035 + -0.2 1,592,035 + +0.1 1,592,035 + +2.7 1,592,035 + +0.2 1,592,035 + -0.4 1,592,035 + +0.5 1,592,035 + +1.3 1,592,035 + -0.2 1,592,037 + +0.6 1,592,037 + -0.9 1,592,037 + -0.7 1,592,037 + +1.1 1,592,037 + -0.7 1,592,037 + -1.9 1,592,039 + -0.4 1,592,039 + -0.8 1,592,039 + -0.0 1,592,039 + +0.4 1,592,039 + -0.3 1,592,039 + -0.3 1,592,039 + -0.7 1,592,112 + +1.3 1,592,158 + +0.6
Or see this region's nucleotide sequence