Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0812 and Psyr_0813 are separated by 37 nucleotides Psyr_0813 and Psyr_0814 are separated by 209 nucleotides Psyr_0814 and Psyr_0815 are separated by 248 nucleotides
Psyr_0812: Psyr_0812 - stress protein, at 917,011 to 917,586
_0812
Psyr_0813: Psyr_0813 - stress protein, at 917,624 to 918,202
_0813
Psyr_0814: Psyr_0814 - Protein of unknown function DUF124, at 918,412 to 919,287
_0814
Psyr_0815: Psyr_0815 - nicotinate-nucleotide pyrophosphorylase (carboxylating), at 919,536 to 920,384
_0815
Position (kb)
918
919
920 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 917.549 kb on + strand at 917.549 kb on + strand at 917.550 kb on - strand at 917.550 kb on - strand at 917.631 kb on + strand at 917.631 kb on + strand at 917.632 kb on - strand at 917.632 kb on - strand at 917.632 kb on - strand at 917.641 kb on + strand at 917.697 kb on + strand, within Psyr_0813 at 917.697 kb on + strand, within Psyr_0813 at 917.698 kb on - strand, within Psyr_0813 at 917.811 kb on - strand, within Psyr_0813 at 918.005 kb on + strand, within Psyr_0813 at 918.005 kb on + strand, within Psyr_0813 at 918.005 kb on + strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.006 kb on - strand, within Psyr_0813 at 918.264 kb on - strand at 918.393 kb on + strand at 918.393 kb on + strand at 918.393 kb on + strand at 918.393 kb on + strand at 918.394 kb on - strand at 918.394 kb on - strand at 918.394 kb on - strand at 918.394 kb on - strand at 918.401 kb on + strand at 918.401 kb on + strand at 918.401 kb on + strand at 918.402 kb on - strand at 918.402 kb on - strand at 918.588 kb on - strand, within Psyr_0814 at 918.588 kb on - strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.052 kb on + strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.053 kb on - strand, within Psyr_0814 at 919.200 kb on + strand at 919.201 kb on - strand at 919.222 kb on + strand at 919.356 kb on - strand at 919.471 kb on + strand at 919.472 kb on - strand at 919.472 kb on - strand at 919.509 kb on + strand at 919.509 kb on + strand at 919.603 kb on + strand at 919.603 kb on + strand at 919.604 kb on - strand at 919.604 kb on - strand at 919.895 kb on - strand, within Psyr_0815 at 919.939 kb on + strand, within Psyr_0815 at 919.939 kb on + strand, within Psyr_0815 at 919.940 kb on - strand, within Psyr_0815 at 919.940 kb on - strand, within Psyr_0815 at 920.238 kb on - strand, within Psyr_0815
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 917,549 + +0.5 917,549 + +0.6 917,550 - -0.6 917,550 - +1.1 917,631 + -1.3 917,631 + -0.5 917,632 - -0.8 917,632 - +0.2 917,632 - -0.1 917,641 + +0.2 917,697 + Psyr_0813 0.13 -0.9 917,697 + Psyr_0813 0.13 +1.3 917,698 - Psyr_0813 0.13 -2.3 917,811 - Psyr_0813 0.32 +0.1 918,005 + Psyr_0813 0.66 -0.3 918,005 + Psyr_0813 0.66 -1.7 918,005 + Psyr_0813 0.66 +2.9 918,006 - Psyr_0813 0.66 -1.2 918,006 - Psyr_0813 0.66 -1.3 918,006 - Psyr_0813 0.66 +2.4 918,006 - Psyr_0813 0.66 -1.5 918,006 - Psyr_0813 0.66 +0.6 918,006 - Psyr_0813 0.66 -1.4 918,006 - Psyr_0813 0.66 +0.3 918,006 - Psyr_0813 0.66 +0.7 918,264 - +0.8 918,393 + +0.7 918,393 + -0.4 918,393 + -2.4 918,393 + +0.1 918,394 - +0.5 918,394 - -0.1 918,394 - -0.5 918,394 - +4.4 918,401 + -0.1 918,401 + +0.3 918,401 + -2.1 918,402 - +0.6 918,402 - -1.6 918,588 - Psyr_0814 0.20 +0.9 918,588 - Psyr_0814 0.20 -1.1 919,052 + Psyr_0814 0.73 -0.5 919,052 + Psyr_0814 0.73 -0.7 919,052 + Psyr_0814 0.73 -1.2 919,052 + Psyr_0814 0.73 +2.3 919,052 + Psyr_0814 0.73 +1.5 919,052 + Psyr_0814 0.73 -2.2 919,052 + Psyr_0814 0.73 -0.6 919,052 + Psyr_0814 0.73 -1.1 919,052 + Psyr_0814 0.73 +0.5 919,052 + Psyr_0814 0.73 +0.5 919,053 - Psyr_0814 0.73 -0.2 919,053 - Psyr_0814 0.73 +3.1 919,053 - Psyr_0814 0.73 +0.2 919,053 - Psyr_0814 0.73 -1.8 919,053 - Psyr_0814 0.73 -1.0 919,053 - Psyr_0814 0.73 -0.1 919,053 - Psyr_0814 0.73 +0.7 919,053 - Psyr_0814 0.73 -0.7 919,053 - Psyr_0814 0.73 +0.4 919,053 - Psyr_0814 0.73 +1.5 919,053 - Psyr_0814 0.73 +0.2 919,200 + -1.1 919,201 - -0.6 919,222 + -0.1 919,356 - -1.5 919,471 + +0.4 919,472 - -1.0 919,472 - +0.4 919,509 + +3.6 919,509 + -2.3 919,603 + -1.1 919,603 + -0.2 919,604 - -0.6 919,604 - -0.5 919,895 - Psyr_0815 0.42 -0.2 919,939 + Psyr_0815 0.47 -0.5 919,939 + Psyr_0815 0.47 +0.7 919,940 - Psyr_0815 0.48 -0.7 919,940 - Psyr_0815 0.48 -0.6 920,238 - Psyr_0815 0.83 -2.5
Or see this region's nucleotide sequence