Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0405 and Psyr_0406 overlap by 4 nucleotides Psyr_0406 and Psyr_0407 overlap by 4 nucleotides Psyr_0407 and Psyr_0408 are separated by 4 nucleotides Psyr_0408 and Psyr_0409 are separated by 114 nucleotides
Psyr_0405: Psyr_0405 - Pilus assembly protein, PilO, at 435,260 to 435,883
_0405
Psyr_0406: Psyr_0406 - Pilus assembly protein, PilQ, at 435,880 to 436,407
_0406
Psyr_0407: Psyr_0407 - type II and III secretion system protein:NolW-like protein:NolW-like protein, at 436,404 to 438,560
_0407
Psyr_0408: Psyr_0408 - shikimate kinase, at 438,565 to 439,083
_0408
Psyr_0409: Psyr_0409 - 3-dehydroquinate synthase, at 439,198 to 440,301
_0409
Position (kb) 436
437
438
439 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 435.512 kb on + strand, within Psyr_0405 at 435.512 kb on + strand, within Psyr_0405 at 435.513 kb on - strand, within Psyr_0405 at 435.513 kb on - strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.680 kb on + strand, within Psyr_0405 at 435.681 kb on + strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.681 kb on - strand, within Psyr_0405 at 435.696 kb on + strand, within Psyr_0405 at 435.857 kb on + strand at 435.857 kb on + strand at 435.858 kb on - strand at 436.034 kb on - strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.048 kb on + strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.049 kb on - strand, within Psyr_0406 at 436.276 kb on + strand, within Psyr_0406 at 436.276 kb on + strand, within Psyr_0406 at 436.277 kb on - strand, within Psyr_0406 at 436.277 kb on - strand, within Psyr_0406 at 436.277 kb on - strand, within Psyr_0406 at 436.277 kb on - strand, within Psyr_0406 at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.424 kb on + strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.425 kb on - strand at 436.433 kb on + strand at 436.433 kb on + strand at 436.434 kb on - strand at 436.729 kb on + strand, within Psyr_0407 at 436.730 kb on - strand, within Psyr_0407 at 436.783 kb on - strand, within Psyr_0407 at 437.295 kb on + strand, within Psyr_0407 at 437.296 kb on - strand, within Psyr_0407 at 437.397 kb on - strand, within Psyr_0407 at 437.397 kb on - strand, within Psyr_0407 at 437.616 kb on + strand, within Psyr_0407 at 437.616 kb on + strand, within Psyr_0407 at 437.616 kb on + strand, within Psyr_0407 at 437.616 kb on + strand, within Psyr_0407 at 437.838 kb on + strand, within Psyr_0407 at 437.839 kb on - strand, within Psyr_0407 at 437.839 kb on - strand, within Psyr_0407 at 437.873 kb on + strand, within Psyr_0407 at 437.873 kb on + strand, within Psyr_0407 at 437.874 kb on - strand, within Psyr_0407 at 437.907 kb on + strand, within Psyr_0407 at 437.907 kb on + strand, within Psyr_0407 at 437.908 kb on - strand, within Psyr_0407 at 437.908 kb on - strand, within Psyr_0407 at 438.294 kb on + strand, within Psyr_0407 at 438.410 kb on + strand at 438.410 kb on + strand at 438.445 kb on + strand at 438.445 kb on + strand at 438.445 kb on + strand at 438.445 kb on + strand at 438.445 kb on + strand at 438.450 kb on + strand at 438.450 kb on + strand at 439.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 435,512 + Psyr_0405 0.40 -0.1 435,512 + Psyr_0405 0.40 -0.4 435,513 - Psyr_0405 0.41 +2.3 435,513 - Psyr_0405 0.41 +2.2 435,680 + Psyr_0405 0.67 +0.5 435,680 + Psyr_0405 0.67 -0.6 435,680 + Psyr_0405 0.67 +1.1 435,681 + Psyr_0405 0.67 +0.4 435,681 - Psyr_0405 0.67 +0.2 435,681 - Psyr_0405 0.67 +0.2 435,681 - Psyr_0405 0.67 +0.3 435,681 - Psyr_0405 0.67 -0.3 435,681 - Psyr_0405 0.67 -0.8 435,681 - Psyr_0405 0.67 -0.5 435,681 - Psyr_0405 0.67 -0.4 435,681 - Psyr_0405 0.67 -0.1 435,681 - Psyr_0405 0.67 +0.1 435,696 + Psyr_0405 0.70 +3.6 435,857 + +0.2 435,857 + -0.4 435,858 - +0.3 436,034 - Psyr_0406 0.29 +0.7 436,048 + Psyr_0406 0.32 -0.9 436,048 + Psyr_0406 0.32 +1.1 436,048 + Psyr_0406 0.32 -1.3 436,049 - Psyr_0406 0.32 -0.7 436,049 - Psyr_0406 0.32 +0.7 436,049 - Psyr_0406 0.32 +0.8 436,276 + Psyr_0406 0.75 +1.0 436,276 + Psyr_0406 0.75 +0.2 436,277 - Psyr_0406 0.75 -0.7 436,277 - Psyr_0406 0.75 +0.9 436,277 - Psyr_0406 0.75 -0.8 436,277 - Psyr_0406 0.75 +0.8 436,424 + -1.1 436,424 + -0.7 436,424 + +0.4 436,424 + +0.9 436,424 + -1.5 436,424 + -0.1 436,424 + -0.9 436,424 + +1.3 436,424 + +1.1 436,425 - +0.9 436,425 - +0.0 436,425 - +0.4 436,425 - -2.5 436,425 - +0.5 436,425 - +3.3 436,425 - -0.1 436,433 + -1.3 436,433 + -1.3 436,434 - +0.8 436,729 + Psyr_0407 0.15 +0.1 436,730 - Psyr_0407 0.15 -0.9 436,783 - Psyr_0407 0.18 +1.0 437,295 + Psyr_0407 0.41 -0.4 437,296 - Psyr_0407 0.41 -0.8 437,397 - Psyr_0407 0.46 +0.5 437,397 - Psyr_0407 0.46 +0.6 437,616 + Psyr_0407 0.56 -0.5 437,616 + Psyr_0407 0.56 +2.9 437,616 + Psyr_0407 0.56 -0.7 437,616 + Psyr_0407 0.56 +1.3 437,838 + Psyr_0407 0.66 +0.5 437,839 - Psyr_0407 0.67 +1.6 437,839 - Psyr_0407 0.67 -1.9 437,873 + Psyr_0407 0.68 +1.0 437,873 + Psyr_0407 0.68 +0.1 437,874 - Psyr_0407 0.68 +2.7 437,907 + Psyr_0407 0.70 -0.6 437,907 + Psyr_0407 0.70 +0.5 437,908 - Psyr_0407 0.70 -0.4 437,908 - Psyr_0407 0.70 +0.0 438,294 + Psyr_0407 0.88 -0.8 438,410 + -1.5 438,410 + -1.0 438,445 + +0.5 438,445 + -0.5 438,445 + +0.7 438,445 + +1.5 438,445 + -0.2 438,450 + +1.3 438,450 + -0.4 439,103 + +1.8
Or see this region's nucleotide sequence