Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0264 and Psyr_0265 are separated by 53 nucleotides Psyr_0265 and Psyr_0266 are separated by 23 nucleotides Psyr_0266 and Psyr_0267 overlap by 4 nucleotides Psyr_0267 and Psyr_0268 are separated by 9 nucleotides
Psyr_0264: Psyr_0264 - Alginate biosynthesis sensor protein KinB, at 280,205 to 281,992
_0264
Psyr_0265: Psyr_0265 - N-acetylmuramoyl-L-alanine amidase, family 2, at 282,046 to 282,897
_0265
Psyr_0266: Psyr_0266 - GGDEF domain protein, at 282,921 to 284,993
_0266
Psyr_0267: Psyr_0267 - Endonuclease/exonuclease/phosphatase, at 284,990 to 285,865
_0267
Psyr_0268: Psyr_0268 - DSBA oxidoreductase, at 285,875 to 286,519
_0268
Position (kb) 282
283
284
285 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 281.942 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.972 kb on + strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.973 kb on - strand at 281.974 kb on + strand at 281.974 kb on + strand at 281.974 kb on + strand at 281.975 kb on - strand at 282.145 kb on + strand, within Psyr_0265 at 282.145 kb on + strand, within Psyr_0265 at 282.458 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.605 kb on + strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.606 kb on - strand, within Psyr_0265 at 282.667 kb on + strand, within Psyr_0265 at 282.667 kb on + strand, within Psyr_0265 at 282.668 kb on - strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.701 kb on + strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.702 kb on - strand, within Psyr_0265 at 282.717 kb on - strand, within Psyr_0265 at 282.717 kb on - strand, within Psyr_0265 at 282.811 kb on + strand, within Psyr_0265 at 282.815 kb on + strand at 282.815 kb on + strand at 282.816 kb on - strand at 282.816 kb on - strand at 282.816 kb on - strand at 282.816 kb on - strand at 282.816 kb on - strand at 282.872 kb on + strand at 282.872 kb on + strand at 282.873 kb on - strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.878 kb on + strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.879 kb on - strand at 282.964 kb on + strand at 282.964 kb on + strand at 282.964 kb on + strand at 282.964 kb on + strand at 282.964 kb on + strand at 282.964 kb on + strand at 282.965 kb on - strand at 282.965 kb on - strand at 282.965 kb on - strand at 282.965 kb on - strand at 282.965 kb on - strand at 282.965 kb on - strand at 283.038 kb on + strand at 283.038 kb on + strand at 283.039 kb on - strand at 283.039 kb on - strand at 283.039 kb on - strand at 283.233 kb on + strand, within Psyr_0266 at 283.234 kb on - strand, within Psyr_0266 at 283.234 kb on - strand, within Psyr_0266 at 283.234 kb on - strand, within Psyr_0266 at 283.535 kb on - strand, within Psyr_0266 at 283.756 kb on + strand, within Psyr_0266 at 283.756 kb on + strand, within Psyr_0266 at 283.756 kb on + strand, within Psyr_0266 at 283.756 kb on + strand, within Psyr_0266 at 283.756 kb on + strand, within Psyr_0266 at 283.756 kb on + strand, within Psyr_0266 at 283.757 kb on - strand, within Psyr_0266 at 283.757 kb on - strand, within Psyr_0266 at 283.757 kb on - strand, within Psyr_0266 at 283.757 kb on - strand, within Psyr_0266 at 283.757 kb on - strand, within Psyr_0266 at 283.863 kb on + strand, within Psyr_0266 at 283.864 kb on - strand, within Psyr_0266 at 283.961 kb on + strand, within Psyr_0266 at 283.961 kb on + strand, within Psyr_0266 at 283.961 kb on + strand, within Psyr_0266 at 283.961 kb on + strand, within Psyr_0266 at 283.961 kb on + strand, within Psyr_0266 at 283.961 kb on + strand, within Psyr_0266 at 283.961 kb on + strand, within Psyr_0266 at 283.962 kb on - strand, within Psyr_0266 at 283.962 kb on - strand, within Psyr_0266 at 283.962 kb on - strand, within Psyr_0266 at 283.962 kb on - strand, within Psyr_0266 at 283.963 kb on + strand, within Psyr_0266 at 283.963 kb on + strand, within Psyr_0266 at 283.963 kb on + strand, within Psyr_0266 at 283.963 kb on + strand, within Psyr_0266 at 283.963 kb on + strand, within Psyr_0266 at 284.362 kb on + strand, within Psyr_0266 at 284.362 kb on + strand, within Psyr_0266 at 284.362 kb on + strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.363 kb on - strand, within Psyr_0266 at 284.367 kb on - strand, within Psyr_0266 at 284.367 kb on - strand, within Psyr_0266 at 284.367 kb on - strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.646 kb on + strand, within Psyr_0266 at 284.647 kb on - strand, within Psyr_0266 at 284.647 kb on - strand, within Psyr_0266 at 284.959 kb on + strand at 284.959 kb on + strand at 284.959 kb on + strand at 284.960 kb on - strand at 284.960 kb on - strand at 284.960 kb on - strand at 284.960 kb on - strand at 285.098 kb on + strand, within Psyr_0267 at 285.185 kb on + strand, within Psyr_0267 at 285.186 kb on - strand, within Psyr_0267 at 285.186 kb on - strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.467 kb on + strand, within Psyr_0267 at 285.705 kb on + strand, within Psyr_0267 at 285.706 kb on - strand, within Psyr_0267 at 285.706 kb on - strand, within Psyr_0267 at 285.706 kb on - strand, within Psyr_0267 at 285.876 kb on + strand at 285.876 kb on + strand at 285.876 kb on + strand at 285.876 kb on + strand at 285.876 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 281,942 + -0.5 281,972 + +0.8 281,972 + +1.2 281,972 + -1.6 281,972 + -0.7 281,972 + -0.4 281,972 + -0.6 281,972 + -0.6 281,972 + -0.3 281,972 + +0.0 281,972 + +0.9 281,972 + -0.1 281,973 - -1.7 281,973 - +0.9 281,973 - -0.7 281,973 - +1.7 281,973 - -0.0 281,973 - +3.3 281,973 - -1.2 281,973 - -0.3 281,973 - +1.8 281,973 - -0.7 281,973 - -0.0 281,973 - -0.6 281,973 - -0.2 281,973 - -0.8 281,974 + +0.5 281,974 + -1.0 281,974 + +0.0 281,975 - -0.0 282,145 + Psyr_0265 0.12 -0.3 282,145 + Psyr_0265 0.12 -0.8 282,458 + Psyr_0265 0.48 -1.7 282,605 + Psyr_0265 0.66 -0.7 282,605 + Psyr_0265 0.66 +0.1 282,605 + Psyr_0265 0.66 +1.6 282,605 + Psyr_0265 0.66 -1.0 282,605 + Psyr_0265 0.66 +0.6 282,605 + Psyr_0265 0.66 +0.1 282,605 + Psyr_0265 0.66 +0.5 282,605 + Psyr_0265 0.66 +0.3 282,605 + Psyr_0265 0.66 -0.0 282,605 + Psyr_0265 0.66 +0.5 282,605 + Psyr_0265 0.66 +1.3 282,606 - Psyr_0265 0.66 +2.7 282,606 - Psyr_0265 0.66 -0.1 282,606 - Psyr_0265 0.66 -0.6 282,606 - Psyr_0265 0.66 -1.3 282,606 - Psyr_0265 0.66 +0.3 282,606 - Psyr_0265 0.66 +0.1 282,606 - Psyr_0265 0.66 -0.5 282,606 - Psyr_0265 0.66 +0.1 282,667 + Psyr_0265 0.73 -0.3 282,667 + Psyr_0265 0.73 -0.4 282,668 - Psyr_0265 0.73 +0.9 282,701 + Psyr_0265 0.77 -0.1 282,701 + Psyr_0265 0.77 +0.0 282,701 + Psyr_0265 0.77 -1.1 282,701 + Psyr_0265 0.77 -0.2 282,701 + Psyr_0265 0.77 +0.0 282,701 + Psyr_0265 0.77 -0.3 282,702 - Psyr_0265 0.77 +0.7 282,702 - Psyr_0265 0.77 -0.0 282,702 - Psyr_0265 0.77 -0.5 282,702 - Psyr_0265 0.77 -0.4 282,702 - Psyr_0265 0.77 -1.1 282,702 - Psyr_0265 0.77 -0.3 282,717 - Psyr_0265 0.79 -0.7 282,717 - Psyr_0265 0.79 -0.8 282,811 + Psyr_0265 0.90 +0.9 282,815 + -0.3 282,815 + -0.3 282,816 - +0.7 282,816 - -0.3 282,816 - +1.1 282,816 - -2.4 282,816 - +1.6 282,872 + -0.5 282,872 + +0.9 282,873 - -1.5 282,878 + -0.0 282,878 + -1.3 282,878 + +0.7 282,878 + -0.6 282,878 + +0.0 282,878 + +0.0 282,878 + +0.6 282,878 + -0.0 282,879 - +1.2 282,879 - +2.9 282,879 - -0.2 282,879 - -2.8 282,879 - -1.4 282,879 - -0.3 282,879 - -0.2 282,879 - -0.1 282,879 - +3.7 282,879 - +0.1 282,879 - -1.0 282,879 - -0.4 282,879 - -0.9 282,964 + -0.2 282,964 + +0.0 282,964 + +0.1 282,964 + +0.0 282,964 + -0.2 282,964 + +0.1 282,965 - -1.4 282,965 - +2.3 282,965 - +0.2 282,965 - -2.2 282,965 - +1.3 282,965 - +1.3 283,038 + +0.3 283,038 + +1.0 283,039 - -1.0 283,039 - +0.1 283,039 - -1.7 283,233 + Psyr_0266 0.15 -0.7 283,234 - Psyr_0266 0.15 +0.2 283,234 - Psyr_0266 0.15 +0.8 283,234 - Psyr_0266 0.15 +1.3 283,535 - Psyr_0266 0.30 -0.3 283,756 + Psyr_0266 0.40 -0.4 283,756 + Psyr_0266 0.40 +0.1 283,756 + Psyr_0266 0.40 -1.5 283,756 + Psyr_0266 0.40 +0.2 283,756 + Psyr_0266 0.40 -0.3 283,756 + Psyr_0266 0.40 +1.3 283,757 - Psyr_0266 0.40 +1.5 283,757 - Psyr_0266 0.40 -0.3 283,757 - Psyr_0266 0.40 +0.3 283,757 - Psyr_0266 0.40 +0.3 283,757 - Psyr_0266 0.40 -0.6 283,863 + Psyr_0266 0.45 +0.1 283,864 - Psyr_0266 0.45 +0.0 283,961 + Psyr_0266 0.50 -1.4 283,961 + Psyr_0266 0.50 +3.3 283,961 + Psyr_0266 0.50 +0.7 283,961 + Psyr_0266 0.50 +2.9 283,961 + Psyr_0266 0.50 -0.8 283,961 + Psyr_0266 0.50 +3.1 283,961 + Psyr_0266 0.50 +0.5 283,962 - Psyr_0266 0.50 -0.4 283,962 - Psyr_0266 0.50 +0.5 283,962 - Psyr_0266 0.50 +4.5 283,962 - Psyr_0266 0.50 -0.2 283,963 + Psyr_0266 0.50 -0.4 283,963 + Psyr_0266 0.50 -1.5 283,963 + Psyr_0266 0.50 -0.2 283,963 + Psyr_0266 0.50 -0.2 283,963 + Psyr_0266 0.50 +0.5 284,362 + Psyr_0266 0.70 +1.4 284,362 + Psyr_0266 0.70 -0.6 284,362 + Psyr_0266 0.70 +2.9 284,363 - Psyr_0266 0.70 +0.1 284,363 - Psyr_0266 0.70 -0.1 284,363 - Psyr_0266 0.70 +0.4 284,363 - Psyr_0266 0.70 +1.3 284,363 - Psyr_0266 0.70 -0.8 284,367 - Psyr_0266 0.70 -0.3 284,367 - Psyr_0266 0.70 -1.5 284,367 - Psyr_0266 0.70 -0.3 284,646 + Psyr_0266 0.83 +0.7 284,646 + Psyr_0266 0.83 +0.1 284,646 + Psyr_0266 0.83 -0.1 284,646 + Psyr_0266 0.83 +1.3 284,647 - Psyr_0266 0.83 +0.5 284,647 - Psyr_0266 0.83 +0.4 284,959 + -1.0 284,959 + +0.4 284,959 + -0.2 284,960 - -0.7 284,960 - +0.9 284,960 - +0.5 284,960 - -4.1 285,098 + Psyr_0267 0.12 +1.1 285,185 + Psyr_0267 0.22 +0.1 285,186 - Psyr_0267 0.22 -0.1 285,186 - Psyr_0267 0.22 -0.2 285,307 - Psyr_0267 0.36 -0.5 285,307 - Psyr_0267 0.36 -0.3 285,307 - Psyr_0267 0.36 -0.7 285,467 + Psyr_0267 0.54 +0.1 285,705 + Psyr_0267 0.82 -0.9 285,706 - Psyr_0267 0.82 +0.0 285,706 - Psyr_0267 0.82 -0.3 285,706 - Psyr_0267 0.82 -0.9 285,876 + -1.6 285,876 + +0.1 285,876 + -0.9 285,876 + -1.1 285,876 + +0.8
Or see this region's nucleotide sequence