Experiment: KB with Hydrastine (1R, 9S) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0237 and Psyr_0238 are separated by 74 nucleotides Psyr_0238 and Psyr_0239 are separated by 25 nucleotides Psyr_0239 and Psyr_0240 overlap by 4 nucleotides Psyr_0240 and Psyr_0241 are separated by 49 nucleotides
Psyr_0237: Psyr_0237 - alanine racemase, at 250,813 to 251,886
_0237
Psyr_0238: Psyr_0238 - Helix-turn-helix motif protein, at 251,961 to 252,560
_0238
Psyr_0239: Psyr_0239 - cytochrome c5, at 252,586 to 252,909
_0239
Psyr_0240: Psyr_0240 - hypothetical protein, at 252,906 to 253,160
_0240
Psyr_0241: Psyr_0241 - conserved hypothetical protein, at 253,210 to 255,141
_0241
Position (kb) 252
253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 251.872 kb on + strand at 251.873 kb on - strand at 251.940 kb on + strand at 251.940 kb on + strand at 251.941 kb on - strand at 252.237 kb on + strand, within Psyr_0238 at 252.237 kb on + strand, within Psyr_0238 at 252.238 kb on - strand, within Psyr_0238 at 252.260 kb on + strand, within Psyr_0238 at 252.260 kb on + strand, within Psyr_0238 at 252.260 kb on + strand, within Psyr_0238 at 252.260 kb on + strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.261 kb on - strand, within Psyr_0238 at 252.262 kb on + strand, within Psyr_0238 at 252.339 kb on + strand, within Psyr_0238 at 252.441 kb on - strand, within Psyr_0238 at 252.441 kb on - strand, within Psyr_0238 at 252.444 kb on + strand, within Psyr_0238 at 252.444 kb on + strand, within Psyr_0238 at 252.444 kb on + strand, within Psyr_0238 at 252.444 kb on + strand, within Psyr_0238 at 252.444 kb on + strand, within Psyr_0238 at 252.444 kb on + strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.445 kb on - strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.468 kb on + strand, within Psyr_0238 at 252.469 kb on - strand, within Psyr_0238 at 252.469 kb on - strand, within Psyr_0238 at 252.469 kb on - strand, within Psyr_0238 at 252.469 kb on - strand, within Psyr_0238 at 252.469 kb on - strand, within Psyr_0238 at 252.471 kb on + strand, within Psyr_0238 at 252.471 kb on + strand, within Psyr_0238 at 252.471 kb on + strand, within Psyr_0238 at 252.472 kb on - strand, within Psyr_0238 at 252.503 kb on + strand at 252.716 kb on + strand, within Psyr_0239 at 252.716 kb on + strand, within Psyr_0239 at 252.716 kb on + strand, within Psyr_0239 at 252.716 kb on + strand, within Psyr_0239 at 252.716 kb on + strand, within Psyr_0239 at 252.716 kb on + strand, within Psyr_0239 at 252.716 kb on + strand, within Psyr_0239 at 252.716 kb on + strand, within Psyr_0239 at 252.717 kb on - strand, within Psyr_0239 at 252.717 kb on - strand, within Psyr_0239 at 253.007 kb on + strand, within Psyr_0240 at 253.031 kb on + strand, within Psyr_0240 at 253.031 kb on + strand, within Psyr_0240 at 253.031 kb on + strand, within Psyr_0240 at 253.031 kb on + strand, within Psyr_0240 at 253.032 kb on - strand, within Psyr_0240 at 253.032 kb on - strand, within Psyr_0240 at 253.032 kb on - strand, within Psyr_0240 at 253.119 kb on + strand, within Psyr_0240 at 253.119 kb on + strand, within Psyr_0240 at 253.119 kb on + strand, within Psyr_0240 at 253.119 kb on + strand, within Psyr_0240 at 253.119 kb on + strand, within Psyr_0240 at 253.120 kb on - strand, within Psyr_0240 at 253.120 kb on - strand, within Psyr_0240 at 253.121 kb on + strand, within Psyr_0240 at 253.216 kb on + strand at 253.331 kb on - strand at 253.331 kb on - strand at 253.372 kb on + strand at 253.372 kb on + strand at 253.372 kb on + strand at 253.373 kb on - strand at 253.582 kb on + strand, within Psyr_0241 at 253.582 kb on + strand, within Psyr_0241 at 253.597 kb on + strand, within Psyr_0241 at 253.597 kb on + strand, within Psyr_0241 at 253.597 kb on + strand, within Psyr_0241 at 253.598 kb on - strand, within Psyr_0241 at 253.598 kb on - strand, within Psyr_0241 at 253.598 kb on - strand, within Psyr_0241 at 253.598 kb on - strand, within Psyr_0241 at 253.598 kb on - strand, within Psyr_0241 at 253.798 kb on + strand, within Psyr_0241 at 253.798 kb on + strand, within Psyr_0241 at 253.798 kb on + strand, within Psyr_0241 at 253.798 kb on + strand, within Psyr_0241 at 253.798 kb on + strand, within Psyr_0241 at 253.798 kb on + strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.799 kb on - strand, within Psyr_0241 at 253.866 kb on + strand, within Psyr_0241 at 253.888 kb on - strand, within Psyr_0241 at 253.888 kb on - strand, within Psyr_0241
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Hydrastine (1R, 9S) 10 mM remove 251,872 + -0.5 251,873 - +0.6 251,940 + -0.6 251,940 + +1.8 251,941 - +0.0 252,237 + Psyr_0238 0.46 -0.4 252,237 + Psyr_0238 0.46 +0.1 252,238 - Psyr_0238 0.46 -0.4 252,260 + Psyr_0238 0.50 -0.9 252,260 + Psyr_0238 0.50 -0.8 252,260 + Psyr_0238 0.50 +0.7 252,260 + Psyr_0238 0.50 +0.9 252,261 - Psyr_0238 0.50 -2.9 252,261 - Psyr_0238 0.50 -1.0 252,261 - Psyr_0238 0.50 +0.2 252,261 - Psyr_0238 0.50 -1.0 252,261 - Psyr_0238 0.50 -1.0 252,261 - Psyr_0238 0.50 +0.2 252,261 - Psyr_0238 0.50 +1.3 252,261 - Psyr_0238 0.50 -2.1 252,261 - Psyr_0238 0.50 +0.4 252,262 + Psyr_0238 0.50 -0.8 252,339 + Psyr_0238 0.63 +1.1 252,441 - Psyr_0238 0.80 -1.0 252,441 - Psyr_0238 0.80 -1.0 252,444 + Psyr_0238 0.81 -0.8 252,444 + Psyr_0238 0.81 -0.9 252,444 + Psyr_0238 0.81 -0.2 252,444 + Psyr_0238 0.81 +0.4 252,444 + Psyr_0238 0.81 -0.5 252,444 + Psyr_0238 0.81 -1.0 252,445 - Psyr_0238 0.81 -0.6 252,445 - Psyr_0238 0.81 +0.9 252,445 - Psyr_0238 0.81 +0.1 252,445 - Psyr_0238 0.81 -0.2 252,445 - Psyr_0238 0.81 +1.8 252,445 - Psyr_0238 0.81 +0.6 252,445 - Psyr_0238 0.81 -1.1 252,445 - Psyr_0238 0.81 -1.7 252,445 - Psyr_0238 0.81 -2.6 252,445 - Psyr_0238 0.81 -0.6 252,445 - Psyr_0238 0.81 -0.1 252,445 - Psyr_0238 0.81 -1.4 252,445 - Psyr_0238 0.81 -0.0 252,445 - Psyr_0238 0.81 -0.2 252,468 + Psyr_0238 0.84 -0.2 252,468 + Psyr_0238 0.84 +0.3 252,468 + Psyr_0238 0.84 -0.9 252,468 + Psyr_0238 0.84 +0.5 252,468 + Psyr_0238 0.84 +0.1 252,468 + Psyr_0238 0.84 +1.7 252,468 + Psyr_0238 0.84 +4.2 252,468 + Psyr_0238 0.84 -0.7 252,469 - Psyr_0238 0.85 -1.5 252,469 - Psyr_0238 0.85 +1.1 252,469 - Psyr_0238 0.85 -0.9 252,469 - Psyr_0238 0.85 +1.5 252,469 - Psyr_0238 0.85 +0.4 252,471 + Psyr_0238 0.85 +0.4 252,471 + Psyr_0238 0.85 +1.6 252,471 + Psyr_0238 0.85 -0.3 252,472 - Psyr_0238 0.85 -0.0 252,503 + +0.6 252,716 + Psyr_0239 0.40 +0.6 252,716 + Psyr_0239 0.40 -0.2 252,716 + Psyr_0239 0.40 -0.3 252,716 + Psyr_0239 0.40 -1.5 252,716 + Psyr_0239 0.40 +0.2 252,716 + Psyr_0239 0.40 -0.6 252,716 + Psyr_0239 0.40 +0.4 252,716 + Psyr_0239 0.40 +0.0 252,717 - Psyr_0239 0.40 +2.1 252,717 - Psyr_0239 0.40 +0.1 253,007 + Psyr_0240 0.40 +0.5 253,031 + Psyr_0240 0.49 -0.7 253,031 + Psyr_0240 0.49 -1.6 253,031 + Psyr_0240 0.49 +1.9 253,031 + Psyr_0240 0.49 -2.5 253,032 - Psyr_0240 0.49 -0.6 253,032 - Psyr_0240 0.49 +0.9 253,032 - Psyr_0240 0.49 +0.1 253,119 + Psyr_0240 0.84 -0.1 253,119 + Psyr_0240 0.84 -0.2 253,119 + Psyr_0240 0.84 +0.6 253,119 + Psyr_0240 0.84 -0.1 253,119 + Psyr_0240 0.84 -0.9 253,120 - Psyr_0240 0.84 +0.5 253,120 - Psyr_0240 0.84 +0.2 253,121 + Psyr_0240 0.84 +0.5 253,216 + -0.2 253,331 - +1.7 253,331 - -0.2 253,372 + -1.1 253,372 + +0.9 253,372 + -1.3 253,373 - +0.4 253,582 + Psyr_0241 0.19 +0.3 253,582 + Psyr_0241 0.19 -1.0 253,597 + Psyr_0241 0.20 +0.6 253,597 + Psyr_0241 0.20 -0.5 253,597 + Psyr_0241 0.20 +0.7 253,598 - Psyr_0241 0.20 -0.0 253,598 - Psyr_0241 0.20 +0.2 253,598 - Psyr_0241 0.20 -1.2 253,598 - Psyr_0241 0.20 -0.0 253,598 - Psyr_0241 0.20 -1.1 253,798 + Psyr_0241 0.30 -0.3 253,798 + Psyr_0241 0.30 -0.2 253,798 + Psyr_0241 0.30 -0.5 253,798 + Psyr_0241 0.30 -1.2 253,798 + Psyr_0241 0.30 -0.9 253,798 + Psyr_0241 0.30 -0.2 253,799 - Psyr_0241 0.30 +1.4 253,799 - Psyr_0241 0.30 -0.0 253,799 - Psyr_0241 0.30 -0.4 253,799 - Psyr_0241 0.30 -1.0 253,799 - Psyr_0241 0.30 +1.4 253,799 - Psyr_0241 0.30 +0.8 253,799 - Psyr_0241 0.30 +1.2 253,799 - Psyr_0241 0.30 +0.3 253,799 - Psyr_0241 0.30 -0.2 253,799 - Psyr_0241 0.30 +0.1 253,799 - Psyr_0241 0.30 +0.8 253,799 - Psyr_0241 0.30 +0.1 253,866 + Psyr_0241 0.34 +2.1 253,888 - Psyr_0241 0.35 -1.1 253,888 - Psyr_0241 0.35 -1.3
Or see this region's nucleotide sequence