Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3684 and Psyr_3685 are separated by 75 nucleotides Psyr_3685 and Psyr_3686 are separated by 14 nucleotides Psyr_3686 and Psyr_3687 are separated by 135 nucleotides
Psyr_3684: Psyr_3684 - NLP/P60, at 4,370,634 to 4,371,362
_3684
Psyr_3685: Psyr_3685 - Silent information regulator protein Sir2, at 4,371,438 to 4,372,181
_3685
Psyr_3686: Psyr_3686 - regulatory inactivation of DnaA Hda protein, at 4,372,196 to 4,372,981
_3686
Psyr_3687: Psyr_3687 - Protein of unknown function UPF0118, at 4,373,117 to 4,374,190
_3687
Position (kb) 4372
4373 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 4371.382 kb on + strand at 4371.382 kb on + strand at 4371.382 kb on + strand at 4371.386 kb on + strand at 4371.387 kb on - strand at 4371.387 kb on - strand at 4371.387 kb on - strand at 4371.387 kb on - strand at 4371.387 kb on - strand at 4371.387 kb on - strand at 4371.400 kb on + strand at 4371.400 kb on + strand at 4371.409 kb on + strand at 4371.409 kb on + strand at 4371.410 kb on - strand at 4371.440 kb on - strand at 4371.450 kb on + strand at 4371.451 kb on - strand at 4371.586 kb on + strand, within Psyr_3685 at 4371.719 kb on + strand, within Psyr_3685 at 4371.720 kb on - strand, within Psyr_3685 at 4371.733 kb on - strand, within Psyr_3685 at 4371.931 kb on + strand, within Psyr_3685 at 4371.986 kb on - strand, within Psyr_3685 at 4371.986 kb on - strand, within Psyr_3685 at 4372.072 kb on + strand, within Psyr_3685 at 4372.072 kb on + strand, within Psyr_3685 at 4372.073 kb on - strand, within Psyr_3685 at 4372.094 kb on - strand, within Psyr_3685 at 4372.197 kb on + strand at 4372.198 kb on - strand at 4372.958 kb on - strand at 4373.118 kb on + strand at 4373.118 kb on + strand at 4373.145 kb on + strand at 4373.164 kb on - strand at 4373.164 kb on - strand at 4373.449 kb on + strand, within Psyr_3687 at 4373.450 kb on - strand, within Psyr_3687 at 4373.450 kb on - strand, within Psyr_3687 at 4373.450 kb on - strand, within Psyr_3687 at 4373.450 kb on - strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.451 kb on + strand, within Psyr_3687 at 4373.452 kb on - strand, within Psyr_3687 at 4373.452 kb on - strand, within Psyr_3687 at 4373.452 kb on - strand, within Psyr_3687 at 4373.452 kb on - strand, within Psyr_3687 at 4373.452 kb on - strand, within Psyr_3687 at 4373.532 kb on + strand, within Psyr_3687 at 4373.532 kb on + strand, within Psyr_3687 at 4373.574 kb on - strand, within Psyr_3687 at 4373.574 kb on - strand, within Psyr_3687 at 4373.692 kb on - strand, within Psyr_3687 at 4373.759 kb on - strand, within Psyr_3687 at 4373.776 kb on + strand, within Psyr_3687 at 4373.804 kb on - strand, within Psyr_3687
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 4,371,382 + +1.6 4,371,382 + -0.2 4,371,382 + +0.8 4,371,386 + -0.4 4,371,387 - -0.3 4,371,387 - +1.4 4,371,387 - -0.1 4,371,387 - +0.1 4,371,387 - -0.6 4,371,387 - -1.7 4,371,400 + -0.4 4,371,400 + +0.4 4,371,409 + +1.3 4,371,409 + -2.1 4,371,410 - +0.4 4,371,440 - +0.2 4,371,450 + -0.2 4,371,451 - -0.6 4,371,586 + Psyr_3685 0.20 -0.2 4,371,719 + Psyr_3685 0.38 +0.4 4,371,720 - Psyr_3685 0.38 -0.4 4,371,733 - Psyr_3685 0.40 -1.6 4,371,931 + Psyr_3685 0.66 +1.4 4,371,986 - Psyr_3685 0.74 +1.6 4,371,986 - Psyr_3685 0.74 -1.8 4,372,072 + Psyr_3685 0.85 -0.2 4,372,072 + Psyr_3685 0.85 -2.2 4,372,073 - Psyr_3685 0.85 -3.8 4,372,094 - Psyr_3685 0.88 +0.4 4,372,197 + +1.4 4,372,198 - -0.0 4,372,958 - -1.6 4,373,118 + -1.9 4,373,118 + +0.2 4,373,145 + +1.4 4,373,164 - -0.1 4,373,164 - -0.2 4,373,449 + Psyr_3687 0.31 +0.6 4,373,450 - Psyr_3687 0.31 -0.2 4,373,450 - Psyr_3687 0.31 -1.3 4,373,450 - Psyr_3687 0.31 -0.2 4,373,450 - Psyr_3687 0.31 -1.0 4,373,451 + Psyr_3687 0.31 +0.6 4,373,451 + Psyr_3687 0.31 +1.0 4,373,451 + Psyr_3687 0.31 +1.2 4,373,451 + Psyr_3687 0.31 +0.5 4,373,451 + Psyr_3687 0.31 +0.8 4,373,451 + Psyr_3687 0.31 -0.9 4,373,451 + Psyr_3687 0.31 +0.6 4,373,451 + Psyr_3687 0.31 -1.4 4,373,451 + Psyr_3687 0.31 -0.1 4,373,451 + Psyr_3687 0.31 -0.1 4,373,451 + Psyr_3687 0.31 +1.4 4,373,451 + Psyr_3687 0.31 +1.4 4,373,452 - Psyr_3687 0.31 +0.2 4,373,452 - Psyr_3687 0.31 +0.4 4,373,452 - Psyr_3687 0.31 -1.0 4,373,452 - Psyr_3687 0.31 -1.2 4,373,452 - Psyr_3687 0.31 -0.3 4,373,532 + Psyr_3687 0.39 -1.2 4,373,532 + Psyr_3687 0.39 -0.2 4,373,574 - Psyr_3687 0.43 +2.4 4,373,574 - Psyr_3687 0.43 -0.2 4,373,692 - Psyr_3687 0.54 +2.3 4,373,759 - Psyr_3687 0.60 -0.1 4,373,776 + Psyr_3687 0.61 +0.6 4,373,804 - Psyr_3687 0.64 +1.2
Or see this region's nucleotide sequence