Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1694 and Psyr_1695 are separated by 84 nucleotides Psyr_1695 and Psyr_1696 are separated by 13 nucleotides Psyr_1696 and Psyr_1697 are separated by 27 nucleotides Psyr_1697 and Psyr_1698 are separated by 11 nucleotides
Psyr_1694: Psyr_1694 - Rhomboid-like protein, at 1,902,263 to 1,902,817
_1694
Psyr_1695: Psyr_1695 - conserved hypothetical protein, at 1,902,902 to 1,903,567
_1695
Psyr_1696: Psyr_1696 - Protein of unknown function DUF350, at 1,903,581 to 1,904,006
_1696
Psyr_1697: Psyr_1697 - conserved hypothetical protein, at 1,904,034 to 1,904,780
_1697
Psyr_1698: Psyr_1698 - Glutathionylspermidine synthase, at 1,904,792 to 1,905,949
_1698
Position (kb) 1903
1904
1905 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1902.617 kb on + strand, within Psyr_1694 at 1902.617 kb on + strand, within Psyr_1694 at 1902.745 kb on + strand, within Psyr_1694 at 1902.745 kb on + strand, within Psyr_1694 at 1902.746 kb on - strand, within Psyr_1694 at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.867 kb on - strand at 1902.867 kb on - strand at 1902.867 kb on - strand at 1902.867 kb on - strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.870 kb on + strand at 1902.870 kb on + strand at 1902.870 kb on + strand at 1902.870 kb on + strand at 1902.871 kb on - strand at 1902.871 kb on - strand at 1902.871 kb on - strand at 1902.902 kb on - strand at 1903.102 kb on + strand, within Psyr_1695 at 1903.102 kb on + strand, within Psyr_1695 at 1903.139 kb on + strand, within Psyr_1695 at 1903.158 kb on - strand, within Psyr_1695 at 1903.200 kb on + strand, within Psyr_1695 at 1903.200 kb on + strand, within Psyr_1695 at 1903.200 kb on + strand, within Psyr_1695 at 1903.200 kb on + strand, within Psyr_1695 at 1903.201 kb on - strand, within Psyr_1695 at 1903.224 kb on - strand, within Psyr_1695 at 1903.305 kb on - strand, within Psyr_1695 at 1903.407 kb on - strand, within Psyr_1695 at 1903.485 kb on + strand, within Psyr_1695 at 1903.534 kb on - strand at 1903.534 kb on - strand at 1903.534 kb on - strand at 1903.598 kb on + strand at 1903.598 kb on + strand at 1903.598 kb on + strand at 1903.599 kb on - strand at 1903.599 kb on - strand at 1903.599 kb on - strand at 1903.599 kb on - strand at 1903.622 kb on - strand at 1903.622 kb on - strand at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.638 kb on + strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.639 kb on - strand, within Psyr_1696 at 1903.683 kb on + strand, within Psyr_1696 at 1903.683 kb on + strand, within Psyr_1696 at 1903.683 kb on + strand, within Psyr_1696 at 1903.684 kb on - strand, within Psyr_1696 at 1903.684 kb on - strand, within Psyr_1696 at 1903.790 kb on + strand, within Psyr_1696 at 1903.796 kb on + strand, within Psyr_1696 at 1903.797 kb on - strand, within Psyr_1696 at 1903.804 kb on - strand, within Psyr_1696 at 1903.804 kb on - strand, within Psyr_1696 at 1903.941 kb on + strand, within Psyr_1696 at 1903.941 kb on + strand, within Psyr_1696 at 1903.941 kb on + strand, within Psyr_1696 at 1904.042 kb on + strand at 1904.042 kb on + strand at 1904.042 kb on + strand at 1904.042 kb on + strand at 1904.042 kb on + strand at 1904.042 kb on + strand at 1904.042 kb on + strand at 1904.042 kb on + strand at 1904.043 kb on - strand at 1904.043 kb on - strand at 1904.043 kb on - strand at 1904.043 kb on - strand at 1904.043 kb on - strand at 1904.059 kb on + strand at 1904.183 kb on + strand, within Psyr_1697 at 1904.183 kb on + strand, within Psyr_1697 at 1904.183 kb on + strand, within Psyr_1697 at 1904.199 kb on + strand, within Psyr_1697 at 1904.199 kb on + strand, within Psyr_1697 at 1904.199 kb on + strand, within Psyr_1697 at 1904.199 kb on + strand, within Psyr_1697 at 1904.199 kb on + strand, within Psyr_1697 at 1904.199 kb on + strand, within Psyr_1697 at 1904.199 kb on + strand, within Psyr_1697 at 1904.241 kb on + strand, within Psyr_1697 at 1904.241 kb on + strand, within Psyr_1697 at 1904.241 kb on + strand, within Psyr_1697 at 1904.241 kb on + strand, within Psyr_1697 at 1904.241 kb on + strand, within Psyr_1697 at 1904.241 kb on + strand, within Psyr_1697 at 1904.242 kb on - strand, within Psyr_1697 at 1904.242 kb on - strand, within Psyr_1697 at 1904.242 kb on - strand, within Psyr_1697 at 1904.242 kb on - strand, within Psyr_1697 at 1904.283 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.310 kb on + strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.311 kb on - strand, within Psyr_1697 at 1904.318 kb on + strand, within Psyr_1697 at 1904.494 kb on - strand, within Psyr_1697 at 1904.508 kb on + strand, within Psyr_1697 at 1904.535 kb on + strand, within Psyr_1697 at 1904.535 kb on + strand, within Psyr_1697 at 1904.538 kb on + strand, within Psyr_1697 at 1904.539 kb on - strand, within Psyr_1697 at 1904.539 kb on - strand, within Psyr_1697 at 1904.539 kb on - strand, within Psyr_1697 at 1904.539 kb on - strand, within Psyr_1697 at 1904.623 kb on - strand, within Psyr_1697 at 1904.901 kb on - strand at 1904.903 kb on + strand at 1904.903 kb on + strand at 1904.903 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 1,902,617 + Psyr_1694 0.64 -0.4 1,902,617 + Psyr_1694 0.64 -1.4 1,902,745 + Psyr_1694 0.87 -1.0 1,902,745 + Psyr_1694 0.87 +0.3 1,902,746 - Psyr_1694 0.87 +0.3 1,902,866 + -0.7 1,902,866 + -0.3 1,902,866 + +1.3 1,902,866 + -3.0 1,902,866 + -1.2 1,902,867 - -2.2 1,902,867 - -1.6 1,902,867 - -0.2 1,902,867 - +0.8 1,902,868 + +0.2 1,902,868 + -0.4 1,902,868 + +0.2 1,902,868 + -1.1 1,902,868 + +0.6 1,902,868 + -0.8 1,902,868 + -0.0 1,902,868 + -0.6 1,902,868 + +0.5 1,902,868 + -2.5 1,902,868 + +0.3 1,902,868 + -0.3 1,902,868 + -1.2 1,902,868 + +1.1 1,902,868 + -0.5 1,902,868 + -1.0 1,902,868 + +2.3 1,902,868 + +0.1 1,902,868 + -0.6 1,902,868 + +3.7 1,902,868 + -1.3 1,902,868 + +1.1 1,902,869 - +3.7 1,902,869 - -0.8 1,902,869 - -1.8 1,902,869 - -1.7 1,902,869 - -1.5 1,902,869 - +2.7 1,902,869 - +1.3 1,902,869 - -1.6 1,902,869 - -1.2 1,902,869 - -2.7 1,902,869 - -2.0 1,902,869 - -1.6 1,902,870 + +0.4 1,902,870 + +0.8 1,902,870 + +0.8 1,902,870 + +1.5 1,902,871 - -1.0 1,902,871 - -1.0 1,902,871 - +0.8 1,902,902 - -1.5 1,903,102 + Psyr_1695 0.30 -1.0 1,903,102 + Psyr_1695 0.30 -0.4 1,903,139 + Psyr_1695 0.36 +0.8 1,903,158 - Psyr_1695 0.38 +0.8 1,903,200 + Psyr_1695 0.45 -1.4 1,903,200 + Psyr_1695 0.45 -0.3 1,903,200 + Psyr_1695 0.45 -0.5 1,903,200 + Psyr_1695 0.45 -1.9 1,903,201 - Psyr_1695 0.45 -0.7 1,903,224 - Psyr_1695 0.48 -1.2 1,903,305 - Psyr_1695 0.61 -0.1 1,903,407 - Psyr_1695 0.76 +0.3 1,903,485 + Psyr_1695 0.88 +1.0 1,903,534 - +0.2 1,903,534 - -0.8 1,903,534 - -2.7 1,903,598 + +0.6 1,903,598 + +0.6 1,903,598 + +0.3 1,903,599 - -0.0 1,903,599 - -1.8 1,903,599 - -0.0 1,903,599 - -0.2 1,903,622 - +2.5 1,903,622 - -1.1 1,903,638 + Psyr_1696 0.13 -0.3 1,903,638 + Psyr_1696 0.13 -1.4 1,903,638 + Psyr_1696 0.13 +2.2 1,903,638 + Psyr_1696 0.13 -0.9 1,903,638 + Psyr_1696 0.13 -0.0 1,903,638 + Psyr_1696 0.13 +1.2 1,903,638 + Psyr_1696 0.13 +0.3 1,903,638 + Psyr_1696 0.13 -1.3 1,903,638 + Psyr_1696 0.13 +1.3 1,903,638 + Psyr_1696 0.13 +0.3 1,903,638 + Psyr_1696 0.13 -1.0 1,903,638 + Psyr_1696 0.13 +1.6 1,903,638 + Psyr_1696 0.13 +0.3 1,903,639 - Psyr_1696 0.14 +1.0 1,903,639 - Psyr_1696 0.14 +0.2 1,903,639 - Psyr_1696 0.14 +0.4 1,903,639 - Psyr_1696 0.14 +1.0 1,903,639 - Psyr_1696 0.14 -1.7 1,903,639 - Psyr_1696 0.14 +0.9 1,903,639 - Psyr_1696 0.14 +0.8 1,903,639 - Psyr_1696 0.14 +0.4 1,903,639 - Psyr_1696 0.14 +0.3 1,903,639 - Psyr_1696 0.14 -0.5 1,903,639 - Psyr_1696 0.14 +0.5 1,903,639 - Psyr_1696 0.14 +0.3 1,903,639 - Psyr_1696 0.14 -0.7 1,903,639 - Psyr_1696 0.14 +1.4 1,903,639 - Psyr_1696 0.14 -1.7 1,903,639 - Psyr_1696 0.14 +0.3 1,903,683 + Psyr_1696 0.24 +0.3 1,903,683 + Psyr_1696 0.24 +0.3 1,903,683 + Psyr_1696 0.24 -0.4 1,903,684 - Psyr_1696 0.24 +0.1 1,903,684 - Psyr_1696 0.24 +0.9 1,903,790 + Psyr_1696 0.49 +2.3 1,903,796 + Psyr_1696 0.50 +0.8 1,903,797 - Psyr_1696 0.51 -0.7 1,903,804 - Psyr_1696 0.52 +1.7 1,903,804 - Psyr_1696 0.52 -0.7 1,903,941 + Psyr_1696 0.85 +1.1 1,903,941 + Psyr_1696 0.85 +0.3 1,903,941 + Psyr_1696 0.85 -0.1 1,904,042 + -1.0 1,904,042 + +1.0 1,904,042 + -0.1 1,904,042 + -0.8 1,904,042 + +0.9 1,904,042 + -1.5 1,904,042 + -0.7 1,904,042 + -1.5 1,904,043 - +0.5 1,904,043 - -0.8 1,904,043 - -0.4 1,904,043 - -0.7 1,904,043 - +0.3 1,904,059 + -0.7 1,904,183 + Psyr_1697 0.20 +0.7 1,904,183 + Psyr_1697 0.20 -0.2 1,904,183 + Psyr_1697 0.20 +1.0 1,904,199 + Psyr_1697 0.22 +0.4 1,904,199 + Psyr_1697 0.22 +0.3 1,904,199 + Psyr_1697 0.22 +0.6 1,904,199 + Psyr_1697 0.22 +0.0 1,904,199 + Psyr_1697 0.22 +1.8 1,904,199 + Psyr_1697 0.22 -1.5 1,904,199 + Psyr_1697 0.22 -1.0 1,904,241 + Psyr_1697 0.28 -1.3 1,904,241 + Psyr_1697 0.28 -0.2 1,904,241 + Psyr_1697 0.28 -0.1 1,904,241 + Psyr_1697 0.28 +1.0 1,904,241 + Psyr_1697 0.28 +1.2 1,904,241 + Psyr_1697 0.28 -0.2 1,904,242 - Psyr_1697 0.28 -0.4 1,904,242 - Psyr_1697 0.28 -0.1 1,904,242 - Psyr_1697 0.28 -0.1 1,904,242 - Psyr_1697 0.28 -0.9 1,904,283 + Psyr_1697 0.33 -0.4 1,904,310 + Psyr_1697 0.37 -2.3 1,904,310 + Psyr_1697 0.37 +0.6 1,904,310 + Psyr_1697 0.37 +2.1 1,904,310 + Psyr_1697 0.37 +0.8 1,904,310 + Psyr_1697 0.37 +2.3 1,904,310 + Psyr_1697 0.37 +0.3 1,904,310 + Psyr_1697 0.37 -3.4 1,904,310 + Psyr_1697 0.37 +0.6 1,904,311 - Psyr_1697 0.37 -0.5 1,904,311 - Psyr_1697 0.37 +1.7 1,904,311 - Psyr_1697 0.37 +0.5 1,904,311 - Psyr_1697 0.37 +0.3 1,904,311 - Psyr_1697 0.37 +1.3 1,904,311 - Psyr_1697 0.37 +0.7 1,904,311 - Psyr_1697 0.37 +1.0 1,904,311 - Psyr_1697 0.37 +0.6 1,904,311 - Psyr_1697 0.37 +0.1 1,904,318 + Psyr_1697 0.38 +0.0 1,904,494 - Psyr_1697 0.62 +0.3 1,904,508 + Psyr_1697 0.63 +0.5 1,904,535 + Psyr_1697 0.67 -0.1 1,904,535 + Psyr_1697 0.67 -1.4 1,904,538 + Psyr_1697 0.67 +0.2 1,904,539 - Psyr_1697 0.68 -1.2 1,904,539 - Psyr_1697 0.68 -1.7 1,904,539 - Psyr_1697 0.68 -0.8 1,904,539 - Psyr_1697 0.68 -0.6 1,904,623 - Psyr_1697 0.79 +0.9 1,904,901 - -0.0 1,904,903 + -1.2 1,904,903 + -0.2 1,904,903 + +1.1
Or see this region's nucleotide sequence