Experiment: KB with Totarol 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1186 and Psyr_1187 are separated by 97 nucleotides Psyr_1187 and Psyr_1188 are separated by 62 nucleotides
Psyr_1186: Psyr_1186 - type III effector HopM1, at 1,340,413 to 1,342,569
_1186
Psyr_1187: Psyr_1187 - type III chaperone protein ShcE, at 1,342,667 to 1,343,056
_1187
Psyr_1188: Psyr_1188 - type III effector protein AvrE1, at 1,343,119 to 1,348,479
_1188
Position (kb)
1342
1343
1344 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1341.866 kb on + strand, within Psyr_1186 at 1341.907 kb on + strand, within Psyr_1186 at 1341.907 kb on + strand, within Psyr_1186 at 1341.907 kb on + strand, within Psyr_1186 at 1341.908 kb on - strand, within Psyr_1186 at 1341.908 kb on - strand, within Psyr_1186 at 1341.908 kb on - strand, within Psyr_1186 at 1341.908 kb on - strand, within Psyr_1186 at 1341.908 kb on - strand, within Psyr_1186 at 1342.027 kb on + strand, within Psyr_1186 at 1342.027 kb on + strand, within Psyr_1186 at 1342.028 kb on - strand, within Psyr_1186 at 1342.028 kb on - strand, within Psyr_1186 at 1342.131 kb on + strand, within Psyr_1186 at 1342.131 kb on + strand, within Psyr_1186 at 1342.131 kb on + strand, within Psyr_1186 at 1342.131 kb on + strand, within Psyr_1186 at 1342.174 kb on + strand, within Psyr_1186 at 1342.175 kb on - strand, within Psyr_1186 at 1342.175 kb on - strand, within Psyr_1186 at 1342.175 kb on - strand, within Psyr_1186 at 1342.175 kb on - strand, within Psyr_1186 at 1342.189 kb on + strand, within Psyr_1186 at 1342.262 kb on + strand, within Psyr_1186 at 1342.263 kb on - strand, within Psyr_1186 at 1342.323 kb on + strand, within Psyr_1186 at 1342.323 kb on + strand, within Psyr_1186 at 1342.324 kb on - strand, within Psyr_1186 at 1342.324 kb on - strand, within Psyr_1186 at 1342.324 kb on - strand, within Psyr_1186 at 1342.324 kb on - strand, within Psyr_1186 at 1342.324 kb on - strand, within Psyr_1186 at 1342.404 kb on - strand at 1342.485 kb on + strand at 1342.485 kb on + strand at 1342.485 kb on + strand at 1342.485 kb on + strand at 1342.485 kb on + strand at 1342.485 kb on + strand at 1342.485 kb on + strand at 1342.486 kb on - strand at 1342.486 kb on - strand at 1342.486 kb on - strand at 1342.486 kb on - strand at 1342.653 kb on - strand at 1342.660 kb on + strand at 1342.660 kb on + strand at 1342.660 kb on + strand at 1342.661 kb on - strand at 1342.661 kb on - strand at 1342.661 kb on - strand at 1342.661 kb on - strand at 1342.672 kb on + strand at 1342.673 kb on - strand at 1342.673 kb on - strand at 1342.736 kb on - strand, within Psyr_1187 at 1342.764 kb on - strand, within Psyr_1187 at 1342.764 kb on - strand, within Psyr_1187 at 1343.015 kb on - strand, within Psyr_1187 at 1343.118 kb on - strand at 1343.118 kb on - strand at 1343.118 kb on - strand at 1343.211 kb on - strand at 1343.241 kb on - strand at 1343.292 kb on + strand at 1343.297 kb on + strand at 1343.297 kb on + strand at 1343.297 kb on + strand at 1343.298 kb on - strand at 1343.298 kb on - strand at 1343.298 kb on - strand at 1343.425 kb on - strand at 1343.553 kb on + strand at 1343.553 kb on + strand at 1343.553 kb on + strand at 1343.554 kb on - strand at 1343.554 kb on - strand at 1343.554 kb on - strand at 1343.554 kb on - strand at 1343.554 kb on - strand at 1343.554 kb on - strand at 1343.554 kb on - strand at 1343.555 kb on + strand at 1343.555 kb on + strand at 1343.555 kb on + strand at 1343.555 kb on + strand at 1343.555 kb on + strand at 1343.555 kb on + strand at 1343.555 kb on + strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.556 kb on - strand at 1343.625 kb on + strand at 1343.626 kb on - strand at 1343.626 kb on - strand at 1343.626 kb on - strand at 1343.626 kb on - strand at 1343.753 kb on + strand, within Psyr_1188 at 1343.753 kb on + strand, within Psyr_1188 at 1343.753 kb on + strand, within Psyr_1188 at 1343.754 kb on - strand, within Psyr_1188 at 1343.754 kb on - strand, within Psyr_1188 at 1343.846 kb on + strand, within Psyr_1188 at 1343.846 kb on + strand, within Psyr_1188 at 1343.966 kb on - strand, within Psyr_1188 at 1343.966 kb on - strand, within Psyr_1188 at 1343.970 kb on + strand, within Psyr_1188
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Totarol 10 mM remove 1,341,866 + Psyr_1186 0.67 +2.9 1,341,907 + Psyr_1186 0.69 +2.3 1,341,907 + Psyr_1186 0.69 +1.9 1,341,907 + Psyr_1186 0.69 +0.7 1,341,908 - Psyr_1186 0.69 -0.5 1,341,908 - Psyr_1186 0.69 +1.1 1,341,908 - Psyr_1186 0.69 -2.0 1,341,908 - Psyr_1186 0.69 -0.1 1,341,908 - Psyr_1186 0.69 +1.5 1,342,027 + Psyr_1186 0.75 +1.5 1,342,027 + Psyr_1186 0.75 +1.1 1,342,028 - Psyr_1186 0.75 +0.5 1,342,028 - Psyr_1186 0.75 +0.5 1,342,131 + Psyr_1186 0.80 +1.0 1,342,131 + Psyr_1186 0.80 +0.5 1,342,131 + Psyr_1186 0.80 -1.7 1,342,131 + Psyr_1186 0.80 -0.5 1,342,174 + Psyr_1186 0.82 +1.6 1,342,175 - Psyr_1186 0.82 -0.4 1,342,175 - Psyr_1186 0.82 -2.0 1,342,175 - Psyr_1186 0.82 -0.4 1,342,175 - Psyr_1186 0.82 -1.2 1,342,189 + Psyr_1186 0.82 -3.1 1,342,262 + Psyr_1186 0.86 +1.0 1,342,263 - Psyr_1186 0.86 -2.2 1,342,323 + Psyr_1186 0.89 -0.6 1,342,323 + Psyr_1186 0.89 +2.3 1,342,324 - Psyr_1186 0.89 -0.3 1,342,324 - Psyr_1186 0.89 -1.4 1,342,324 - Psyr_1186 0.89 +2.9 1,342,324 - Psyr_1186 0.89 +0.3 1,342,324 - Psyr_1186 0.89 -1.3 1,342,404 - +0.7 1,342,485 + +0.3 1,342,485 + +0.7 1,342,485 + -1.0 1,342,485 + -0.2 1,342,485 + -0.8 1,342,485 + -0.5 1,342,485 + +0.1 1,342,486 - +0.3 1,342,486 - +0.3 1,342,486 - +0.4 1,342,486 - +1.6 1,342,653 - -0.2 1,342,660 + +0.4 1,342,660 + +0.2 1,342,660 + -0.1 1,342,661 - -0.1 1,342,661 - -1.5 1,342,661 - -1.6 1,342,661 - +1.3 1,342,672 + -0.2 1,342,673 - -1.1 1,342,673 - +0.3 1,342,736 - Psyr_1187 0.18 +3.3 1,342,764 - Psyr_1187 0.25 +0.1 1,342,764 - Psyr_1187 0.25 +0.3 1,343,015 - Psyr_1187 0.89 +0.1 1,343,118 - -1.2 1,343,118 - -0.4 1,343,118 - +0.3 1,343,211 - +0.5 1,343,241 - +0.8 1,343,292 + +0.1 1,343,297 + +1.7 1,343,297 + +1.1 1,343,297 + -1.7 1,343,298 - +0.0 1,343,298 - -1.0 1,343,298 - +0.3 1,343,425 - +0.5 1,343,553 + +0.8 1,343,553 + +2.9 1,343,553 + -1.2 1,343,554 - -0.2 1,343,554 - -0.2 1,343,554 - -0.7 1,343,554 - +0.2 1,343,554 - +0.2 1,343,554 - -0.2 1,343,554 - +0.1 1,343,555 + -0.1 1,343,555 + -1.2 1,343,555 + -1.1 1,343,555 + -1.0 1,343,555 + -1.2 1,343,555 + -0.1 1,343,555 + +0.6 1,343,556 - +0.3 1,343,556 - +1.7 1,343,556 - -0.7 1,343,556 - +2.9 1,343,556 - -1.8 1,343,556 - +0.3 1,343,556 - -1.0 1,343,556 - -0.3 1,343,556 - -1.1 1,343,556 - +2.3 1,343,556 - +2.7 1,343,556 - -0.4 1,343,556 - +0.3 1,343,625 + +0.0 1,343,626 - +0.8 1,343,626 - -2.2 1,343,626 - +0.1 1,343,626 - +0.5 1,343,753 + Psyr_1188 0.12 +0.4 1,343,753 + Psyr_1188 0.12 -0.3 1,343,753 + Psyr_1188 0.12 +1.3 1,343,754 - Psyr_1188 0.12 -0.5 1,343,754 - Psyr_1188 0.12 +0.6 1,343,846 + Psyr_1188 0.14 -3.3 1,343,846 + Psyr_1188 0.14 -0.1 1,343,966 - Psyr_1188 0.16 -1.0 1,343,966 - Psyr_1188 0.16 +0.2 1,343,970 + Psyr_1188 0.16 -0.1
Or see this region's nucleotide sequence