Experiment: KB with Avocadyne 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3244 and Psyr_3245 are separated by 86 nucleotides Psyr_3245 and Psyr_3246 are separated by 20 nucleotides
Psyr_3244: Psyr_3244 - Phospholipase D/Transphosphatidylase, at 3,884,704 to 3,886,047
_3244
Psyr_3245: Psyr_3245 - ATP-dependent DNA ligase LigD phosphoesterase module / ATP-dependent DNA ligase LigD polymerase module, at 3,886,134 to 3,888,734
_3245
Psyr_3246: Psyr_3246 - Ku, at 3,888,755 to 3,889,660
_3246
Position (kb)
3886
3887
3888
3889 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3885.137 kb on - strand, within Psyr_3244 at 3885.180 kb on + strand, within Psyr_3244 at 3885.181 kb on - strand, within Psyr_3244 at 3885.184 kb on + strand, within Psyr_3244 at 3885.185 kb on - strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.508 kb on + strand, within Psyr_3244 at 3885.508 kb on + strand, within Psyr_3244 at 3885.508 kb on + strand, within Psyr_3244 at 3885.508 kb on + strand, within Psyr_3244 at 3885.509 kb on - strand, within Psyr_3244 at 3885.656 kb on - strand, within Psyr_3244 at 3885.656 kb on - strand, within Psyr_3244 at 3885.661 kb on + strand, within Psyr_3244 at 3885.662 kb on - strand, within Psyr_3244 at 3885.662 kb on - strand, within Psyr_3244 at 3885.662 kb on - strand, within Psyr_3244 at 3886.076 kb on + strand at 3886.076 kb on + strand at 3886.077 kb on - strand at 3886.077 kb on - strand at 3886.100 kb on + strand at 3886.100 kb on + strand at 3886.101 kb on - strand at 3886.101 kb on - strand at 3886.378 kb on + strand at 3886.703 kb on + strand, within Psyr_3245 at 3886.704 kb on - strand, within Psyr_3245 at 3886.758 kb on + strand, within Psyr_3245 at 3886.759 kb on - strand, within Psyr_3245 at 3886.759 kb on - strand, within Psyr_3245 at 3887.019 kb on + strand, within Psyr_3245 at 3887.020 kb on - strand, within Psyr_3245 at 3887.313 kb on - strand, within Psyr_3245 at 3887.365 kb on + strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.443 kb on + strand, within Psyr_3245 at 3887.515 kb on + strand, within Psyr_3245 at 3887.515 kb on + strand, within Psyr_3245 at 3887.516 kb on - strand, within Psyr_3245 at 3887.516 kb on - strand, within Psyr_3245 at 3887.516 kb on - strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.728 kb on + strand, within Psyr_3245 at 3887.729 kb on - strand, within Psyr_3245 at 3887.972 kb on - strand, within Psyr_3245 at 3888.364 kb on + strand, within Psyr_3245 at 3888.365 kb on - strand, within Psyr_3245 at 3888.365 kb on - strand, within Psyr_3245 at 3888.433 kb on + strand, within Psyr_3245 at 3888.433 kb on + strand, within Psyr_3245 at 3888.450 kb on - strand, within Psyr_3245 at 3888.660 kb on - strand at 3888.660 kb on - strand at 3888.712 kb on + strand at 3888.713 kb on - strand at 3889.261 kb on + strand, within Psyr_3246 at 3889.293 kb on + strand, within Psyr_3246 at 3889.294 kb on - strand, within Psyr_3246 at 3889.382 kb on + strand, within Psyr_3246 at 3889.382 kb on + strand, within Psyr_3246 at 3889.383 kb on - strand, within Psyr_3246 at 3889.440 kb on - strand, within Psyr_3246 at 3889.446 kb on + strand, within Psyr_3246 at 3889.446 kb on + strand, within Psyr_3246 at 3889.446 kb on + strand, within Psyr_3246 at 3889.447 kb on - strand, within Psyr_3246 at 3889.447 kb on - strand, within Psyr_3246 at 3889.610 kb on + strand at 3889.611 kb on - strand at 3889.611 kb on - strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadyne 10 mM remove 3,885,137 - Psyr_3244 0.32 +1.2 3,885,180 + Psyr_3244 0.35 -0.0 3,885,181 - Psyr_3244 0.35 -0.2 3,885,184 + Psyr_3244 0.36 -3.4 3,885,185 - Psyr_3244 0.36 +2.7 3,885,260 + Psyr_3244 0.41 +0.9 3,885,260 + Psyr_3244 0.41 -0.7 3,885,260 + Psyr_3244 0.41 +1.7 3,885,260 + Psyr_3244 0.41 +0.5 3,885,508 + Psyr_3244 0.60 +0.4 3,885,508 + Psyr_3244 0.60 -1.8 3,885,508 + Psyr_3244 0.60 -1.6 3,885,508 + Psyr_3244 0.60 +0.1 3,885,509 - Psyr_3244 0.60 -0.4 3,885,656 - Psyr_3244 0.71 -0.1 3,885,656 - Psyr_3244 0.71 +0.8 3,885,661 + Psyr_3244 0.71 -1.5 3,885,662 - Psyr_3244 0.71 +0.3 3,885,662 - Psyr_3244 0.71 -0.1 3,885,662 - Psyr_3244 0.71 +2.4 3,886,076 + -1.3 3,886,076 + +1.1 3,886,077 - -0.4 3,886,077 - +1.0 3,886,100 + -0.3 3,886,100 + -0.0 3,886,101 - +0.6 3,886,101 - -0.3 3,886,378 + +1.0 3,886,703 + Psyr_3245 0.22 +1.5 3,886,704 - Psyr_3245 0.22 +0.4 3,886,758 + Psyr_3245 0.24 +0.4 3,886,759 - Psyr_3245 0.24 +0.5 3,886,759 - Psyr_3245 0.24 -1.2 3,887,019 + Psyr_3245 0.34 -0.0 3,887,020 - Psyr_3245 0.34 +1.4 3,887,313 - Psyr_3245 0.45 -0.2 3,887,365 + Psyr_3245 0.47 -1.2 3,887,366 - Psyr_3245 0.47 -1.7 3,887,443 + Psyr_3245 0.50 -1.0 3,887,515 + Psyr_3245 0.53 +2.9 3,887,515 + Psyr_3245 0.53 +0.3 3,887,516 - Psyr_3245 0.53 +0.2 3,887,516 - Psyr_3245 0.53 +3.0 3,887,516 - Psyr_3245 0.53 +1.6 3,887,725 + Psyr_3245 0.61 -2.2 3,887,725 + Psyr_3245 0.61 +0.0 3,887,725 + Psyr_3245 0.61 -0.6 3,887,725 + Psyr_3245 0.61 -0.6 3,887,725 + Psyr_3245 0.61 +0.3 3,887,725 + Psyr_3245 0.61 +0.9 3,887,728 + Psyr_3245 0.61 -0.1 3,887,729 - Psyr_3245 0.61 +0.3 3,887,972 - Psyr_3245 0.71 -1.2 3,888,364 + Psyr_3245 0.86 +0.8 3,888,365 - Psyr_3245 0.86 +0.2 3,888,365 - Psyr_3245 0.86 +0.3 3,888,433 + Psyr_3245 0.88 +0.9 3,888,433 + Psyr_3245 0.88 +0.3 3,888,450 - Psyr_3245 0.89 -1.6 3,888,660 - -0.1 3,888,660 - -1.6 3,888,712 + +0.5 3,888,713 - +0.6 3,889,261 + Psyr_3246 0.56 +1.3 3,889,293 + Psyr_3246 0.59 +0.2 3,889,294 - Psyr_3246 0.59 +0.3 3,889,382 + Psyr_3246 0.69 -0.1 3,889,382 + Psyr_3246 0.69 -0.2 3,889,383 - Psyr_3246 0.69 -0.2 3,889,440 - Psyr_3246 0.76 -0.6 3,889,446 + Psyr_3246 0.76 +2.3 3,889,446 + Psyr_3246 0.76 -0.4 3,889,446 + Psyr_3246 0.76 +1.0 3,889,447 - Psyr_3246 0.76 -0.3 3,889,447 - Psyr_3246 0.76 -0.7 3,889,610 + -0.5 3,889,611 - -0.2 3,889,611 - -1.3 3,889,612 + +1.3 3,889,612 + +0.7 3,889,613 - -0.2
Or see this region's nucleotide sequence