Experiment: KB with Avocadyne 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3222 and Psyr_3223 are separated by 3 nucleotides Psyr_3223 and Psyr_3224 overlap by 11 nucleotides Psyr_3224 and Psyr_3225 are separated by 0 nucleotides
Psyr_3222: Psyr_3222 - Polysaccharide biosynthesis protein, at 3,859,580 to 3,861,094
_3222
Psyr_3223: Psyr_3223 - Glycosyl transferase, family 2, at 3,861,098 to 3,862,033
_3223
Psyr_3224: Psyr_3224 - Low molecular weight phosphotyrosine protein phosphatase, at 3,862,023 to 3,862,475
_3224
Psyr_3225: Psyr_3225 - Protein-tyrosine kinase, at 3,862,476 to 3,864,683
_3225
Position (kb)
3861
3862
3863 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3860.558 kb on + strand, within Psyr_3222 at 3860.559 kb on - strand, within Psyr_3222 at 3860.633 kb on + strand, within Psyr_3222 at 3860.678 kb on + strand, within Psyr_3222 at 3860.678 kb on + strand, within Psyr_3222 at 3860.679 kb on - strand, within Psyr_3222 at 3860.687 kb on + strand, within Psyr_3222 at 3860.687 kb on + strand, within Psyr_3222 at 3860.688 kb on - strand, within Psyr_3222 at 3860.688 kb on - strand, within Psyr_3222 at 3860.693 kb on + strand, within Psyr_3222 at 3860.694 kb on - strand, within Psyr_3222 at 3860.720 kb on + strand, within Psyr_3222 at 3860.721 kb on - strand, within Psyr_3222 at 3860.721 kb on - strand, within Psyr_3222 at 3860.775 kb on - strand, within Psyr_3222 at 3861.065 kb on + strand at 3861.066 kb on - strand at 3861.066 kb on - strand at 3861.088 kb on + strand at 3861.111 kb on + strand at 3861.159 kb on + strand at 3861.159 kb on + strand at 3861.159 kb on + strand at 3861.160 kb on - strand at 3861.160 kb on - strand at 3861.171 kb on - strand at 3861.333 kb on - strand, within Psyr_3223 at 3861.351 kb on - strand, within Psyr_3223 at 3861.405 kb on - strand, within Psyr_3223 at 3861.431 kb on + strand, within Psyr_3223 at 3861.492 kb on - strand, within Psyr_3223 at 3861.492 kb on - strand, within Psyr_3223 at 3861.492 kb on - strand, within Psyr_3223 at 3861.503 kb on + strand, within Psyr_3223 at 3861.503 kb on + strand, within Psyr_3223 at 3861.504 kb on - strand, within Psyr_3223 at 3861.691 kb on + strand, within Psyr_3223 at 3861.692 kb on - strand, within Psyr_3223 at 3861.692 kb on - strand, within Psyr_3223 at 3861.692 kb on - strand, within Psyr_3223 at 3861.692 kb on - strand, within Psyr_3223 at 3861.692 kb on - strand, within Psyr_3223 at 3861.692 kb on - strand, within Psyr_3223 at 3861.720 kb on - strand, within Psyr_3223 at 3861.852 kb on - strand, within Psyr_3223 at 3861.852 kb on - strand, within Psyr_3223 at 3861.854 kb on + strand, within Psyr_3223 at 3861.854 kb on + strand, within Psyr_3223 at 3861.855 kb on - strand, within Psyr_3223 at 3861.855 kb on - strand, within Psyr_3223 at 3861.855 kb on - strand, within Psyr_3223 at 3861.882 kb on - strand, within Psyr_3223 at 3861.882 kb on - strand, within Psyr_3223 at 3861.887 kb on + strand, within Psyr_3223 at 3861.887 kb on + strand, within Psyr_3223 at 3862.031 kb on + strand at 3862.032 kb on - strand at 3862.032 kb on - strand at 3862.117 kb on + strand, within Psyr_3224 at 3862.117 kb on + strand, within Psyr_3224 at 3862.118 kb on - strand, within Psyr_3224 at 3862.118 kb on - strand, within Psyr_3224 at 3862.118 kb on - strand, within Psyr_3224 at 3862.118 kb on - strand, within Psyr_3224 at 3862.118 kb on - strand, within Psyr_3224 at 3862.118 kb on - strand, within Psyr_3224 at 3862.121 kb on + strand, within Psyr_3224 at 3862.122 kb on - strand, within Psyr_3224 at 3862.122 kb on - strand, within Psyr_3224 at 3862.410 kb on + strand, within Psyr_3224 at 3862.411 kb on - strand, within Psyr_3224 at 3862.639 kb on - strand at 3862.639 kb on - strand at 3862.664 kb on - strand at 3862.664 kb on - strand at 3862.842 kb on + strand, within Psyr_3225 at 3862.842 kb on + strand, within Psyr_3225
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadyne 10 mM remove 3,860,558 + Psyr_3222 0.65 +1.3 3,860,559 - Psyr_3222 0.65 +0.7 3,860,633 + Psyr_3222 0.70 +1.3 3,860,678 + Psyr_3222 0.72 -1.2 3,860,678 + Psyr_3222 0.72 -0.0 3,860,679 - Psyr_3222 0.73 +0.8 3,860,687 + Psyr_3222 0.73 +0.6 3,860,687 + Psyr_3222 0.73 +1.9 3,860,688 - Psyr_3222 0.73 +0.6 3,860,688 - Psyr_3222 0.73 +1.4 3,860,693 + Psyr_3222 0.73 +1.3 3,860,694 - Psyr_3222 0.74 -1.9 3,860,720 + Psyr_3222 0.75 +0.4 3,860,721 - Psyr_3222 0.75 +1.9 3,860,721 - Psyr_3222 0.75 -1.1 3,860,775 - Psyr_3222 0.79 +0.7 3,861,065 + +0.3 3,861,066 - +0.3 3,861,066 - -1.3 3,861,088 + +1.0 3,861,111 + -2.5 3,861,159 + -0.1 3,861,159 + -1.0 3,861,159 + -0.4 3,861,160 - -1.3 3,861,160 - -0.0 3,861,171 - -2.5 3,861,333 - Psyr_3223 0.25 +0.0 3,861,351 - Psyr_3223 0.27 +1.1 3,861,405 - Psyr_3223 0.33 +0.6 3,861,431 + Psyr_3223 0.36 -0.3 3,861,492 - Psyr_3223 0.42 +0.9 3,861,492 - Psyr_3223 0.42 -0.0 3,861,492 - Psyr_3223 0.42 +2.3 3,861,503 + Psyr_3223 0.43 +3.3 3,861,503 + Psyr_3223 0.43 +0.1 3,861,504 - Psyr_3223 0.43 -0.9 3,861,691 + Psyr_3223 0.63 +0.5 3,861,692 - Psyr_3223 0.63 +0.2 3,861,692 - Psyr_3223 0.63 -0.3 3,861,692 - Psyr_3223 0.63 +0.7 3,861,692 - Psyr_3223 0.63 +0.3 3,861,692 - Psyr_3223 0.63 -0.1 3,861,692 - Psyr_3223 0.63 -1.2 3,861,720 - Psyr_3223 0.66 +0.4 3,861,852 - Psyr_3223 0.81 -2.2 3,861,852 - Psyr_3223 0.81 -0.2 3,861,854 + Psyr_3223 0.81 +0.3 3,861,854 + Psyr_3223 0.81 -1.8 3,861,855 - Psyr_3223 0.81 -2.3 3,861,855 - Psyr_3223 0.81 +2.1 3,861,855 - Psyr_3223 0.81 +0.8 3,861,882 - Psyr_3223 0.84 +0.0 3,861,882 - Psyr_3223 0.84 -0.3 3,861,887 + Psyr_3223 0.84 -1.4 3,861,887 + Psyr_3223 0.84 +0.6 3,862,031 + +0.4 3,862,032 - +1.0 3,862,032 - +0.1 3,862,117 + Psyr_3224 0.21 -0.3 3,862,117 + Psyr_3224 0.21 -0.5 3,862,118 - Psyr_3224 0.21 -0.3 3,862,118 - Psyr_3224 0.21 -2.2 3,862,118 - Psyr_3224 0.21 +0.5 3,862,118 - Psyr_3224 0.21 +0.0 3,862,118 - Psyr_3224 0.21 +1.3 3,862,118 - Psyr_3224 0.21 +4.3 3,862,121 + Psyr_3224 0.22 +1.7 3,862,122 - Psyr_3224 0.22 -0.0 3,862,122 - Psyr_3224 0.22 -0.4 3,862,410 + Psyr_3224 0.85 +0.6 3,862,411 - Psyr_3224 0.86 +1.3 3,862,639 - -0.2 3,862,639 - +0.1 3,862,664 - -0.6 3,862,664 - -0.6 3,862,842 + Psyr_3225 0.17 +0.5 3,862,842 + Psyr_3225 0.17 +1.4
Or see this region's nucleotide sequence