Experiment: KB with Avocadyne 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1724 and Psyr_1725 are separated by 86 nucleotides Psyr_1725 and Psyr_1726 are separated by 314 nucleotides Psyr_1726 and Psyr_1727 are separated by 387 nucleotides Psyr_1727 and Psyr_1728 are separated by 84 nucleotides
Psyr_1724: Psyr_1724 - glutamate dehydrogenase (NAD), at 1,953,126 to 1,957,982
_1724
Psyr_1725: Psyr_1725 - Protein of unknown function DUF204, at 1,958,069 to 1,958,605
_1725
Psyr_1726: Psyr_1726 - aconitase, at 1,958,920 to 1,961,535
_1726
Psyr_1727: Psyr_1727 - Protein of unknown function DUF1289, at 1,961,923 to 1,962,393
_1727
Psyr_1728: Psyr_1728 - tRNA--hydroxylase, at 1,962,478 to 1,963,089
_1728
Position (kb)
1958
1959
1960
1961
1962 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.920 kb on + strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.921 kb on - strand at 1957.992 kb on - strand at 1958.052 kb on + strand at 1958.052 kb on + strand at 1958.053 kb on - strand at 1958.053 kb on - strand at 1958.385 kb on + strand, within Psyr_1725 at 1958.385 kb on + strand, within Psyr_1725 at 1958.386 kb on - strand, within Psyr_1725 at 1958.386 kb on - strand, within Psyr_1725 at 1958.386 kb on - strand, within Psyr_1725 at 1958.386 kb on - strand, within Psyr_1725 at 1958.386 kb on - strand, within Psyr_1725 at 1958.555 kb on - strand at 1958.645 kb on + strand at 1958.646 kb on - strand at 1958.693 kb on + strand at 1958.765 kb on + strand at 1958.765 kb on + strand at 1958.824 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.845 kb on + strand at 1958.846 kb on - strand at 1958.846 kb on - strand at 1958.847 kb on + strand at 1958.847 kb on + strand at 1958.848 kb on - strand at 1958.848 kb on - strand at 1958.851 kb on + strand at 1958.851 kb on + strand at 1958.851 kb on + strand at 1958.851 kb on + strand at 1958.917 kb on + strand at 1958.917 kb on + strand at 1958.918 kb on - strand at 1958.918 kb on - strand at 1958.921 kb on + strand at 1958.921 kb on + strand at 1958.921 kb on + strand at 1958.922 kb on - strand at 1958.922 kb on - strand at 1958.922 kb on - strand at 1958.922 kb on - strand at 1958.993 kb on + strand at 1961.795 kb on - strand at 1961.847 kb on - strand at 1961.932 kb on + strand at 1961.932 kb on + strand at 1962.352 kb on + strand at 1962.352 kb on + strand at 1962.353 kb on - strand at 1962.353 kb on - strand at 1962.353 kb on - strand at 1962.438 kb on + strand at 1962.439 kb on - strand at 1962.439 kb on - strand at 1962.439 kb on - strand at 1962.441 kb on - strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.477 kb on + strand at 1962.478 kb on - strand at 1962.478 kb on - strand at 1962.482 kb on - strand at 1962.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadyne 10 mM remove 1,957,920 + -1.9 1,957,920 + -1.3 1,957,920 + +1.1 1,957,920 + -0.3 1,957,920 + -1.3 1,957,920 + +0.3 1,957,921 - +1.0 1,957,921 - +0.8 1,957,921 - +0.3 1,957,921 - +1.3 1,957,921 - +0.8 1,957,921 - +0.3 1,957,921 - +3.9 1,957,921 - -0.8 1,957,921 - +1.5 1,957,992 - -1.5 1,958,052 + -0.6 1,958,052 + +0.1 1,958,053 - +0.2 1,958,053 - +0.3 1,958,385 + Psyr_1725 0.59 +0.6 1,958,385 + Psyr_1725 0.59 -1.4 1,958,386 - Psyr_1725 0.59 +0.8 1,958,386 - Psyr_1725 0.59 -0.8 1,958,386 - Psyr_1725 0.59 +1.7 1,958,386 - Psyr_1725 0.59 +1.3 1,958,386 - Psyr_1725 0.59 +1.6 1,958,555 - -1.0 1,958,645 + +0.9 1,958,646 - +0.0 1,958,693 + -0.7 1,958,765 + +1.3 1,958,765 + -0.5 1,958,824 + +3.3 1,958,845 + -0.9 1,958,845 + -0.7 1,958,845 + +0.6 1,958,845 + -0.1 1,958,846 - +0.6 1,958,846 - -0.4 1,958,847 + +0.2 1,958,847 + +0.1 1,958,848 - +0.3 1,958,848 - -1.0 1,958,851 + -0.4 1,958,851 + -0.5 1,958,851 + +2.2 1,958,851 + -0.7 1,958,917 + -3.1 1,958,917 + -1.5 1,958,918 - -0.7 1,958,918 - -0.1 1,958,921 + -0.4 1,958,921 + -1.2 1,958,921 + +1.5 1,958,922 - +0.3 1,958,922 - -0.5 1,958,922 - -0.7 1,958,922 - +1.8 1,958,993 + -2.1 1,961,795 - -0.7 1,961,847 - +0.4 1,961,932 + -2.7 1,961,932 + -2.6 1,962,352 + +0.5 1,962,352 + -0.7 1,962,353 - -0.5 1,962,353 - +0.1 1,962,353 - -0.1 1,962,438 + +0.5 1,962,439 - -0.5 1,962,439 - +2.9 1,962,439 - -1.2 1,962,441 - -0.7 1,962,477 + +0.7 1,962,477 + -0.5 1,962,477 + -0.5 1,962,477 + +2.6 1,962,477 + -0.0 1,962,478 - -1.5 1,962,478 - +2.4 1,962,482 - -0.7 1,962,482 - +0.7
Or see this region's nucleotide sequence