Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_0205

Experiment: KB with Avocadyne 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0203 and Psyr_0204 overlap by 4 nucleotidesPsyr_0204 and Psyr_0205 are separated by 3 nucleotidesPsyr_0205 and Psyr_0206 are separated by 208 nucleotides Psyr_0203: Psyr_0203 - outer membrane transport energization protein TonB, at 218,563 to 219,309 _0203 Psyr_0204: Psyr_0204 - outer membrane transport energization protein ExbD, at 219,306 to 219,731 _0204 Psyr_0205: Psyr_0205 - outer membrane transport energization protein ExbB, at 219,735 to 220,697 _0205 Psyr_0206: Psyr_0206 - conserved hypothetical protein, at 220,906 to 221,754 _0206 Position (kb) 219 220 221Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 219.334 kb on - strandat 220.813 kb on + strandat 220.813 kb on + strandat 220.817 kb on - strandat 220.817 kb on - strandat 220.831 kb on - strandat 220.834 kb on + strandat 220.835 kb on - strandat 221.101 kb on + strand, within Psyr_0206at 221.101 kb on + strand, within Psyr_0206at 221.101 kb on + strand, within Psyr_0206at 221.102 kb on - strand, within Psyr_0206at 221.102 kb on - strand, within Psyr_0206at 221.102 kb on - strand, within Psyr_0206at 221.110 kb on + strand, within Psyr_0206at 221.110 kb on + strand, within Psyr_0206at 221.110 kb on + strand, within Psyr_0206at 221.110 kb on + strand, within Psyr_0206at 221.110 kb on + strand, within Psyr_0206at 221.110 kb on + strand, within Psyr_0206at 221.110 kb on + strand, within Psyr_0206at 221.111 kb on - strand, within Psyr_0206at 221.111 kb on - strand, within Psyr_0206at 221.111 kb on - strand, within Psyr_0206at 221.158 kb on + strand, within Psyr_0206at 221.158 kb on + strand, within Psyr_0206at 221.158 kb on + strand, within Psyr_0206at 221.159 kb on - strand, within Psyr_0206at 221.159 kb on - strand, within Psyr_0206at 221.159 kb on - strand, within Psyr_0206at 221.159 kb on - strand, within Psyr_0206at 221.305 kb on + strand, within Psyr_0206at 221.305 kb on + strand, within Psyr_0206at 221.305 kb on + strand, within Psyr_0206at 221.305 kb on + strand, within Psyr_0206at 221.305 kb on + strand, within Psyr_0206at 221.305 kb on + strand, within Psyr_0206at 221.306 kb on - strand, within Psyr_0206at 221.306 kb on - strand, within Psyr_0206at 221.306 kb on - strand, within Psyr_0206at 221.306 kb on - strand, within Psyr_0206at 221.306 kb on - strand, within Psyr_0206at 221.306 kb on - strand, within Psyr_0206at 221.306 kb on - strand, within Psyr_0206at 221.386 kb on - strand, within Psyr_0206at 221.390 kb on - strand, within Psyr_0206at 221.472 kb on + strand, within Psyr_0206at 221.473 kb on - strand, within Psyr_0206at 221.473 kb on - strand, within Psyr_0206at 221.473 kb on - strand, within Psyr_0206at 221.611 kb on + strand, within Psyr_0206at 221.612 kb on - strand, within Psyr_0206at 221.676 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Avocadyne 10 mM
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219,334 - -0.6
220,813 + -1.9
220,813 + -3.4
220,817 - +0.0
220,817 - +1.6
220,831 - -2.0
220,834 + -2.8
220,835 - +0.4
221,101 + Psyr_0206 0.23 +0.9
221,101 + Psyr_0206 0.23 +2.3
221,101 + Psyr_0206 0.23 -0.4
221,102 - Psyr_0206 0.23 +1.7
221,102 - Psyr_0206 0.23 +0.6
221,102 - Psyr_0206 0.23 -0.9
221,110 + Psyr_0206 0.24 -1.7
221,110 + Psyr_0206 0.24 -0.3
221,110 + Psyr_0206 0.24 -2.8
221,110 + Psyr_0206 0.24 -0.9
221,110 + Psyr_0206 0.24 -0.4
221,110 + Psyr_0206 0.24 +0.1
221,110 + Psyr_0206 0.24 +0.6
221,111 - Psyr_0206 0.24 +0.5
221,111 - Psyr_0206 0.24 +0.0
221,111 - Psyr_0206 0.24 -1.8
221,158 + Psyr_0206 0.30 +1.8
221,158 + Psyr_0206 0.30 +1.1
221,158 + Psyr_0206 0.30 +0.8
221,159 - Psyr_0206 0.30 +0.0
221,159 - Psyr_0206 0.30 -0.3
221,159 - Psyr_0206 0.30 +0.7
221,159 - Psyr_0206 0.30 -0.5
221,305 + Psyr_0206 0.47 +0.1
221,305 + Psyr_0206 0.47 -0.3
221,305 + Psyr_0206 0.47 +0.6
221,305 + Psyr_0206 0.47 -0.8
221,305 + Psyr_0206 0.47 +0.5
221,305 + Psyr_0206 0.47 -1.4
221,306 - Psyr_0206 0.47 -0.5
221,306 - Psyr_0206 0.47 +1.8
221,306 - Psyr_0206 0.47 -1.2
221,306 - Psyr_0206 0.47 -0.8
221,306 - Psyr_0206 0.47 +1.2
221,306 - Psyr_0206 0.47 +0.9
221,306 - Psyr_0206 0.47 +1.0
221,386 - Psyr_0206 0.57 -1.7
221,390 - Psyr_0206 0.57 +0.4
221,472 + Psyr_0206 0.67 +1.1
221,473 - Psyr_0206 0.67 -1.0
221,473 - Psyr_0206 0.67 -0.7
221,473 - Psyr_0206 0.67 -0.1
221,611 + Psyr_0206 0.83 -0.5
221,612 - Psyr_0206 0.83 +0.5
221,676 + +0.7

Or see this region's nucleotide sequence